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Anti mouse igg 7076

Manufactured by Cell Signaling Technology
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Anti-mouse IgG (7076) is a secondary antibody product designed for use in immunological assays. It is a purified goat anti-mouse IgG antibody conjugated to horseradish peroxidase (HRP). This product can be used to detect and quantify mouse immunoglobulins in a variety of applications.

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Lab products found in correlation

Anti rabbit igg 7074, by Cell Signaling Technology (24 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (4 mentions) Ripa buffer, by Thermo Fisher Scientific (3 mentions) Anti talin antibody clone 8d4 t3287, by Merck Group (3 mentions) Anti cd41 antibody ab134131, by Abcam (3 mentions) Rabbit anti e cadherin, by Cell Signaling Technology (2 mentions) Penicillin streptomycin, by Thermo Fisher Scientific (2 mentions) Dht elisa kit, by Sunlong Biotech (2 mentions) Ecl western blotting substrate, by Promega (2 mentions) Chemidoc xrs system, by Bio-Rad (2 mentions) Ab290, by Abcam (2 mentions) Streptomycin, by Thermo Fisher Scientific (2 mentions) Penicillin, by Thermo Fisher Scientific (2 mentions) Anti cytochrome c sc 13156, by Santa Cruz Biotechnology (2 mentions) Anti cox 4 4850, by Cell Signaling Technology (2 mentions) Horseradish peroxidase conjugated anti rabbit immunoglobulin g igg 7074, by Cell Signaling Technology (2 mentions) Protease inhibitor cocktail, by Merck Group (2 mentions) Hrp linked anti rabbit igg 7074, by Cell Signaling Technology (2 mentions) Goat anti mouse igg 115 035 003, by Jackson ImmunoResearch (2 mentions) Hrp conjugated donkey anti goat igg v8051, by Promega (2 mentions)

Spelling variants of this product

Anti-mouse (#7076) (54 citations) Mouse IgG (7076) (4 citations) IgG (Mouse) (3 citations) Anti‐mouse IgG‐HRP (7076S; (3 citations) Horse anti-mouse IgG-HRP antibody (7076S) (2 citations) Horse anti-Mouse IgG-HRP (7076S) (2 citations) Goat anti-mouse IgG-HRP (7076S, (2 citations)

44 protocols using anti mouse igg 7076

1

Protein Extraction and Western Blot Analysis

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Proteins were extracted from cell lines using RIPA Buffer (Thermo) according to the manufacturer's protocol. Protein extraction and western blot analysis with chemiluminescent detection were as described [50 (link)]. The following antibodies and dilution factors were used: FOXM1 rabbit polyclonal antibody (13147-1-AP, 1:800, Proteintech), cyclin B1 (CCNB1) rabbit polyclonal antibody (4138P, 1:800, Cell Signaling), anti-rabbit vimentin (5741, 1:100 Cell Signaling), anti-rabbit E-cadherin (3195, 1:200, Cell Signaling), mouse GAPDH monoclonal antibody (MA5-15738, 1:2000, Sigma), anti-rabbit IgG conjugated to horse radish peroxidase (7074S, 1:2000, Cell Signaling), and anti-mouse IgG (7076S, 1:2,000, Cell Signaling).
Tan X., Fu Y., Chen L., Lee W., Lai Y., Rezaei K., Tabbara S., Latham P., Teal C.B., Man Y.G., Siegel R.S., Brem R.F, & Fu S.W. (2015). miR-671-5p inhibits epithelial-to-mesenchymal transition by downregulating FOXM1 expression in breast cancer. Oncotarget, 7(1), 293-307.
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2

Western Blot Analysis of Cell Signaling

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Cell lysates were prepared using the RIPA Buffer (ThermoFisher Sci) according to the manufacturer’s protocol, and Western blot analysis with chemiluminescent detection was performed using the ProteinSimple imaging system as described [21 (link)]. The following antibodies and dilution factors were used: FOXM1 rabbit polyclonal antibody (13147-1-AP, 1:800, Proteintech), anti-rabbit vimentin (5741, 1:200 Cell Signaling), anti-rabbit E-cadherin (3195, 1:400, Cell Signaling), anti-rabbit beta-actin (4970 s, 1:2000, Cell Signaling), anti-rabbit IgG conjugated to horseradish peroxidase (7074S, 1:2000, Cell Signaling), and anti-mouse IgG (7076S, 1:2,000, Cell Signaling).
Tan X., Li Z., Ren S., Rezaei K., Pan Q., Goldstein A.T., Macri C.J., Cao D., Brem R.F, & Fu S.W. (2019). Dynamically decreased miR-671-5p expression is associated with oncogenic transformation and radiochemoresistance in breast cancer. Breast Cancer Research : BCR, 21, 89.
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3

Triptolide-Biotin Synthesis and Analysis

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Triptolide was purchased from Solarbio Life Science (ST8290, Beijing, China). Triptolide-Biotin were synthesized as described in Supplementary Information. Compounds were dissolved in DMSO at a concentration of 100 mM and stored at -20°C. Ferrostatin-1 (Fer-1) was purchased from Sigma-Aldrich (SML0583, United States). Anti-SLC7A11 rabbit polyclonal antibody (386116) and anti-GLS2 rabbit polyclonal antibody (163996) were purchased from Zen Bio (Chengdu, Sichuan, China). Anti-GPX4 mouse monoclonal antibody (67763-1-Ig) was purchased from Proteintech (Wuhan, Hubei, China). Anti-4-HNE rabbit polyclonal antibody (ab46545) and anti-β-actin rabbit polyclonal antibody (ab8227) were purchased from Abcam (Cambridge, UK). Anti-Nrf2 rabbit monoclonal antibody (12721S), anti-HO-1 rabbit monoclonal antibody (5853S), anti-GAPDH rabbit monoclonal antibody (5174S), secondary antibody anti-rabbit IgG (7074S), and anti-mouse IgG (7076S) were purchased from cell signaling technology (Danvers, MA, USA).
Liu X., Chen C., Han D., Zhou W., Cui Y., Tang X., Xiao C., Wang Y, & Gao Y. (2022). SLC7A11/GPX4 Inactivation-Mediated Ferroptosis Contributes to the Pathogenesis of Triptolide-Induced Cardiotoxicity. Oxidative Medicine and Cellular Longevity, 2022, 3192607.
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4

Antibody Validation for Protein Analysis

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Antibodies used in the work included the rabbit antibodies against HA tag (Cell Signaling #C29F4), PFKP (Cell Signaling #5412S), beta-Actin (Cell Signaling #13E5), BRD4 (Bethyl Laboratories #A301-985A100), H3K27ac (Abcam #ab4729), cleaved caspase 3 and 7 (Cell Signaling #9664 and 8438), as well as the mouse antibodies against Flag tag (Sigma #F1804), YY1 (Santa Cruz #SC7341; for blotting), YY1 (Atlas Antibodies #HPA001119; for IHC), TADA2A (Active Motif #61334), and α-Tubulin (Sigma #T9026). Anti-FLAG M2 Magnetic Beads (Sigma #M8823) was obtained from Sigma and used for immunoprecipitation. HRP-linked secondary antibodies, either anti-mouse IgG (#7076S) or anti-rabbit IgG (#7074S), were obtained from Cell Signaling.
Xu C., Tsai Y.H., Galbo PM J.r., Gong W., Storey A.J., Xu Y., Byrum S.D., Xu L., Whang Y.E., Parker J.S., Mackintosh S.G., Edmondson R.D., Tackett A.J., Huang J., Zheng D., Earp H.S., Wang G.G, & Cai L. (2021). Cistrome analysis of YY1 uncovers a regulatory axis of YY1:BRD2/4-PFKP during tumorigenesis of advanced prostate cancer. Nucleic Acids Research, 49(9), 4971-4988.
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5

Antibody Source and Dilution Protocol

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Antibodies against the following proteins/epitopes were purchased from the indicated sources: GAPDH (sc-47724), His tag (sc-8036), GST (sc-138), p53 (DO1, sc-126), MDM2 (sc-965), DAXX (sc-8043), α-synuclein (syn211, sc-12767) (Santa Cruz Biotechnology); Flag tag (14793) and DAXX (4533S) (Cell Signaling Technology); p53 (PAb1620, OP33; PAb240, OP29), MDM2 (OP46) (Calbiochem); HA tag (ab137838) and luciferase (ab21176) (Abcam); and β-Amyloid 1–42 (Aβ42, 805509) (BioLegend). HRP-conjugated anti-rabbit IgG (7074S) and anti-mouse IgG (7076S) antibodies were purchased from Cell Signaling Technology; IRDye 800CW (926–32211, anti-Rabbit) and IRDye 680RD (926–68070, anti-Mouse) secondary antibodies from Li-Cor; anti-Flag M2 Affinity Gel (A2220), 3xFlag peptide (F4799), firefly luciferase (L9420), and Aβ42 (A9810) from Sigma Aldrich; HSP70 (human HSP72, ADI-NSP-555), HSP40 (human HDJ1, ADI-SPP-400), and ATP regeneration solution (BML-EW9810–0100) from Enzo Life Sciences; and 6xHis-ubiquitin (U-530), UBE1 (E-304), and UBE2D2 (E2–622) from Boston Biochem. α-Synuclein (RP-003, RP-001) was purchased from Proteos. For western blot, anti-DAXX, p53, MDM2, and α-synuclein antibodies were used at 1:1,000 dilution, IRDye 800CW and IRDye 680RD at 1:10,000 dilution, and all other antibodies at 1:2,000 dilution.
Huang L., Agrawal T., Zhu G., Yu S., Tao L., Lin J., Marmorstein R., Shorter J, & Yang X. (2021). DAXX represents a new type of protein-folding enabler. Nature, 597(7874), 132-137.
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6

Glucose-Dependent Protein Expression Analysis

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Approximately 1 × 106 cells were seeded in 100mm dishes (Corning, NY, USA). They were exposed to increasing and decreasing glucose concentrations for 24, 48 and 72 h. Then, the protein was isolated using the M-PER Mammalian Protein Extraction Reagent (Thermo Fisher Scientific, Waltham, MA, USA) as per the manufacturer’s instructions. Isolated protein was then quantified using the Bradford reagent (BioRad Laboratories, Hercules, CA, USA), and 40 µg of protein from each sample was run on a 10% SDS-PAGE gel. The gel was then transferred onto a nitrocellulose membrane (BioRad Laboratories, Hercules, CA, USA) and blocked in 5% skim milk powder (Sigma Aldrich, St. Louis, MO, USA) for 1 h at room temperature (RT). Following blocking, the blots were incubated with primary antibodies against ACE2 (1:1000; A12737; Abclonal, Woburn, MA, USA), TMPRSS2 (1:1000; ab214462; Abcam, Cambridge, UK), and the loading control of anti-beta-actin (1:2000; Sigma Aldrich, St. Louis, MO, USA). On the following day, blots were washed and incubated with the appropriate secondary antibodies (anti-mouse IgG [7076S] and anti-rabbit IgG [7074S]; 1:1000; Cell Signaling Technology, Danvers, MA, USA) before visualization using Clarity Western ECL substrate reagent (BioRad Laboratories, Hercules, CA, USA) in a BioRad chemi-doc imaging system (BioRad Laboratories, Hercules, CA, USA).
Srivastava A, & Mussa B.M. (2022). Effects of Varying Glucose Concentrations on ACE2′s Hypothalamic Expression and Its Potential Relation to COVID-19-Associated Neurological Dysfunction. International Journal of Molecular Sciences, 23(17), 9645.
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7

Temozolomide-Based Combination Therapy

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Temozolomide (TMZ) (99.9% purity), glyceryl monooleate (GMO) and freez (Poloxamer 407) were purchased from ACEM biochemical, Shanghai, China. Bovine serum albumin and curcumin were obtained from BioFroxx Germany. The primary antibodies for P-gp (22336-1-AP) and GAPDH (60004-1-Ig) were purchased from proteintech (MA, USA), while secondary antibodies anti-rabbit IgG (7074S) and anti-mouse IgG (7076S) were obtained from Cell Signaling Technology, Inc.
Almoshari Y., Iqbal H., Razzaq A., Ali Ahmad K., Khan M.K., Saeed Alqahtani S., Hadi Sultan M, & Ali Khan B. (2022). Development of nanocubosomes co-loaded with dual anticancer agents curcumin and temozolomide for effective Colon cancer therapy. Drug Delivery, 29(1), 2633-2643.
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8

Screening Modulators of Stemness Pathways

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MK-0752 was purchased from APExBIO (Houston, TX, USA). RO4929097, PF-03084014, LY3039478, and LY450139 were obtained from Selleckchem (Houston, TX, USA). BMS-708163 and BMS-906024 were purchased from Tocris Bioscience (Bristol, UK) and MilliporeSigma (Burlington, MA, USA), respectively. CellTiter 96® AQueous one solution reagent containing a tetrazolium compound [3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, inner salt; MTS] was purchased from Promega (Madison, WI, USA). Propidium iodide (P4864) was purchased from Sigma-Aldrich (St. Louis, MO, USA). UCP1 antibody (ab10983) and goat polyclonal secondary antibody to rabbit immunoglobulin G (IgG) - H&L (Alexa Fluor® 488, ab150077) were obtained from Abcam (Cambridge, UK). CCAAT/enhancer-binding protein alpha (C/EBPα, SC-61), and β-Actin (SC-47778) antibodies were purchased from Santa Cruz Biotechnology (Dallas, TX, USA). Cleaved caspase-3 (9661S), peroxisome proliferator-activated receptor gamma (PPARγ, 81B8), and horseradish peroxidase (HRP)-conjugated secondary antibodies, including anti-rabbit IgG (7074S) and anti-mouse IgG (7076S), were purchased from Cell Signaling Technology (Danvers, MA, USA). All other reagents and solvents were purchased from Sigma-Aldrich.
Huang D., Qiu J., Kuang S, & Deng M. (2020). In Vitro Evaluation of Clinical Candidates of γ-Secretase Inhibitors: Effects on Notch Inhibition and Promoting Beige Adipogenesis and Mitochondrial Biogenesis. Pharmaceutical research, 37(10), 185.
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9

Molecular Mechanisms of miR-145-5p in Autophagy

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SalB was purchased form Herbpurify (purity > 99%; CAS No. 121521–90-2, Cat No. 115939–25-8, Lot No. 190322 R). The primary antibodies against microtubule-associated protein light chain 3 (LC3, ab192890) were obtained from Abcam. The primary antibodies against phosphatidylinositol 3-kinase (PI3K, 4257s), AKT (9272s), phosphorylated-AKT (P-AKT Ser473, 9271s), beclin1 (3758s), anti-mouse IgG (7076s), and anti-rabbit IgG (7074s), horseradish peroxidase-linked Antibody were purchased from Cell Signaling Technology. The antibody against glyceraldehyde-3-phosphate dehydrogenase (GAPDH, sc-32233) was from Santa Cruz Biotechnology. miR-145-5p antagomir, inhibitor, mimic and miR negative control were purchased from RiboBio.
Chen J., Hu Q., Luo Y., Luo L., Lin H., Chen D., Xu Y., Liu B., He Y., Liang C., Liu Y., Zhou J, & Wu J. (2022). Salvianolic acid B attenuates membranous nephropathy by activating renal autophagy via microRNA-145-5p/phosphatidylinositol 3-kinase/AKT pathway. Bioengineered, 13(5), 13956-13969.
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10

Protein Extraction and Western Blot Analysis

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Cells were harvested, washed and the total proteins were extracted in ice-cold RIPA buffer supplemented with proteases and phosphatases inhibitors (Sigma-Aldrich, Sigma, USA). The protein concentration was quantified using the Pierce Protein Assay Kit (Pierce, 23227). For western blot assay, an equal amount of protein was separated by electrophoresis on SDS-PAGE gel and transferred to polyvinylidene difluoride membranes (Merck Millipore, USA). Membranes were incubated with 5% non-fat milk in TBS (Tris-buffered saline) buffer and probed with various primary antibodies HIF1α (Abcam, ab113642, 1:1000); SRGN (Abcam, ab211515, 1:500); β-actin, GAPDH, β-Tubulin (Beijing Ray Antibody Biotech, RM2003, RM2002, RM2001, 1:5000). The membranes were washed three times with TBST and incubated with horseradish peroxidase (HRP)-conjugated secondary antibody (Cell Signaling Technology, anti-rabbit IgG 7074S or antimouse IgG 7076S, 1:5000). Specific antibody binding was detected using enhanced chemiluminescence detection reagents (Pierce).
Xu Y., Xu J., Yang Y., Zhu L., Li X, & Zhao W. (2018). SRGN Promotes Colorectal Cancer Metastasis as a Critical Downstream Target of HIF-1α. Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, 48(6).
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