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Ly6g microbead kit

Manufactured by Miltenyi Biotec
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The Ly6G microbead kit is a tool for the isolation of Ly6G-positive cells from mouse or rat cell suspensions. It utilizes magnetic microbeads coated with an antibody specific for the Ly6G antigen, allowing for the efficient separation of Ly6G-expressing cells from the sample.

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Anti-Ly6G microbead kit (38 citations) MicroBead kits (12 citations) Ly6G microbeads (9 citations) Anti-Ly6G MicroBeads UltraPure kit (2 citations)

2 protocols using ly6g microbead kit

1

PMN-MDSC Isolation and Differentiation

Cited 1 time
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We performed experiments using PMN-MDSC obtained by two different procedures:

Purification of spleen derived PMN-MDSC were performed by immunomagnetic positive selection from mice carrying 21-day Lewis lung cell carcinoma (LLC) tumors with Ly6G microbead kit (Miltenyi RRID: AB_2895065) according to manufacturer’s recommendations.

Differentiated PMN-MDSC obtained from murine bone marrow were generated as previously described (57 (link)). Briefly, mononuclear precursors were obtained from bone marrow of 8-12 week-old female C57BL/6J mice (RRID:IMSR_JAX:000664), and cultured in complete medium for 24 hours. Non-adherent cells were recovered 24 post-extraction and reseeded. These cells were incubated for 7 days with 50% conditioned medium obtained from 3-day culture of 8 x 105 genetically modified Lewis lung carcinoma cells, and 50% complete medium. Adherent cells were harvested with PBS with 1 mM EDTA, and were used for flow cytometry analyses.

Ruiz-Fernández de Córdoba B., Moreno H., Valencia K., Perurena N., Ruedas P., Walle T., Pezonaga-Torres A., Hinojosa J., Guruceaga E., Pineda-Lucena A., Abengózar-Muela M., Cochonneau D., Zandueta C., Martínez-Canarias S., Teijeira Á., Ajona D., Ortiz-Espinosa S., Morales X., Ortiz de Solórzano C., Santisteban M., Ramos-García L.I., Guembe L., Strnad V., Heymann D., Hervás-Stubbs S., Pío R., Rodríguez-Ruiz M.E., de Andrea C.E., Vicent S., Melero I., Lecanda F, & Martínez-Monge R. (2022). Tumor ENPP1 (CD203a)/Haptoglobin Axis Exploits Myeloid-Derived Suppressor Cells to Promote Post-Radiotherapy Local Recurrence in Breast Cancer. Cancer Discovery, 12(5), 1356-1377.
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2

Isolation and Generation of Murine PMN-MDSC

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
We performed experiments using PMN-MDSC obtained by two different procedures:

Purification of spleen-derived PMN-MDSC was performed by immunomagnetic positive selection from mice carrying 21-day Lewis lung cell carcinoma tumors with Ly6G microbead kit (Miltenyi RRID: AB_2895065) according to the manufacturer's recommendations.

Differentiated PMN-MDSC obtained from murine bone marrow were generated as previously described (57 (link)). Briefly, mononuclear precursors were obtained from bone marrow of 8- to 12-week-old female C57BL/6J mice (RRID:IMSR_JAX:000664) and cultured in complete medium for 24 hours. Nonadherent cells were recovered 24 hours after extraction and reseeded. These cells were incubated for 7 days with 50% CM obtained from 3-day culture of 8 × 105 genetically modified Lewis lung carcinoma cells and 50% complete medium. Adherent cells were harvested with PBS with 1 mmol/L EDTA and were used for flow cytometry analyses.

Ruiz-Fernández de Córdoba B., Moreno H., Valencia K., Perurena N., Ruedas P., Walle T., Pezonaga-Torres A., Hinojosa J., Guruceaga E., Pineda-Lucena A., Abengózar-Muela M., Cochonneau D., Zandueta C., Martínez-Canarias S., Teijeira Á., Ajona D., Ortiz-Espinosa S., Morales X., Ortiz de Solórzano C., Santisteban M., Ramos-García L.I., Guembe L., Strnad V., Heymann D., Hervás-Stubbs S., Pío R., Rodríguez-Ruiz M.E., de Andrea C.E., Vicent S., Melero I., Lecanda F, & Martínez-Monge R. (2022). Tumor ENPP1 (CD203a)/Haptoglobin Axis Exploits Myeloid-Derived Suppressor Cells to Promote Post-Radiotherapy Local Recurrence in Breast Cancer. Cancer Discovery, 12(5), 1356-1377.
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