Donkey anti goat hrp conjugated antibody ft 1i7890

Manufactured by Interchim

Donkey anti-goat HRP conjugated antibody (FT-1I7890) is a laboratory reagent used to detect and quantify the presence of goat proteins in samples. This antibody is conjugated with horseradish peroxidase (HRP), which allows for colorimetric or chemiluminescent detection of the target antigen.

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Lab products found in correlation

Mouse monoclonal anti tubulin antibody clone 236 10501, by Thermo Fisher Scientific (2 mentions) Alexa fluor 488 conjugated anti rabbit igg, by Abcam (2 mentions) Goat anti rabbit igg hrp, by Cell Signaling Technology (1 mentions) Rabbit anti β actin polyclonal antibody, by Merck Group (1 mentions) Cy3 conjugated anti mouse igg, by Proteintech (1 mentions) Rabbit monoclonal anti pd l1 clone sp142, by Roche (1 mentions) Goat anti rabbit igg hrp 7074s, by Cell Signaling Technology (1 mentions)

2 protocols using donkey anti goat hrp conjugated antibody ft 1i7890

Antibody Sources and Characterization

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The rabbit polyclonal anti-SPARC antibody (15274-1-AP) was purchased from Proteintech. The mouse monoclonal anti-human SPARC (clone AON-5031, sc-73472), the rabbit polyclonal anti-human cath-D antibody (H-75, sc-10725), and the mouse monoclonal anti-human cath-D (clone C-5, sc-377124) antibodies were purchased from Santa Cruz Biotechnology. The mouse monoclonal anti-human cath-D antibody (clone 49, #610801) was purchased from BD Transduction Laboratories^TM, and the goat polyclonal anti-mouse cath-D (AF1029) from R&D Systems. The anti-human cath-D antibodies M1G8 and D7E3 were previously described 19 (link). The mouse monoclonal anti-tubulin antibody (clone 236-10501, #A11126) was from Thermo Fisher Scientific, the mouse monoclonal anti-Myc (clone 9B11) from Ozyme, and the rabbit polyclonal anti-β actin antibody (#A2066) from Sigma-Aldrich. The horse anti-mouse immunoglobulin G (IgG)-horseradish peroxidase (#7076), and goat anti-rabbit IgG-HRP (#7074S) secondary antibodies were from Cell Signaling Technology. The donkey anti-goat HRP conjugated antibody (FT-1I7890) was from Interchim. The Alexa Fluor 488-conjugated anti-rabbit IgG (#Ab150077) was purchased from Abcam, and the Cy3-conjugated anti-mouse IgG (#SA00009.1) from Proteintech. Hoechst 33342 (#FP-BB1340) was from Interchim FluoProbes.

Alcaraz L.B., Mallavialle A., David T., Derocq D., Delolme F., Dieryckx C., Mollevi C., Boissière-Michot F., Simony-Lafontaine J., Du Manoir S., Huesgen P.F., Overall C.M., Tartare-Deckert S., Jacot W., Chardès T., Guiu S., Roger P., Reinheckel T., Moali C, & Liaudet-Coopman E. (2021). A 9-kDa matricellular SPARC fragment released by cathepsin D exhibits pro-tumor activity in the triple-negative breast cancer microenvironment. Theranostics, 11(13), 6173-6192.

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Comprehensive Antibody Panel for Protein Detection

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The rabbit polyclonal anti‐SPARC (15274‐1‐AP) and the mouse monoclonal anti‐periostin (clone No 1A11A3) antibodies were purchased from Proteintech. The mouse monoclonal anti‐SPARC (clone AON‐5031, sc‐73 472) and the mouse monoclonal anti‐HSC70 (clone B‐6, sc‐7298) antibodies were purchased from Santa Cruz Biotechnology. The mouse monoclonal anti‐tubulin antibody (clone 236‐10 501, #A11126) was from Thermo Fisher Scientific. The mouse monoclonal anti‐cytokeratin 5/6 antibody (clone 6D5/16 B4) was from Dako. The mouse monoclonal anti‐epidermal growth factor receptor (EGFR) antibody (clone 31G7) was from inVitroGen. The mouse monoclonal anti‐PD‐1 (clone MRQ‐22), and the mouse monoclonal anti‐CD163 (clone 10D6) antibodies were from BioSB. The rabbit monoclonal anti‐PD‐L1 (clone SP142) was from Roche. The horse anti‐mouse immunoglobulin G (IgG)‐horseradish peroxidase (#7076), and goat anti‐rabbit IgG‐HRP (#7074 S) secondary antibodies were from Cell Signaling Technology. The donkey anti‐goat HRP conjugated antibody (FT‐1I7890) was from Interchim. The Alexa Fluor 488‐conjugated anti‐rabbit IgG (#Ab150077) was purchased from Abcam, and the Alexa Fluor 594‐conjugated anti‐mouse IgG (711‐585‐152) from ImmunoResearch Laboratories. Hoechst 33342 (#FP‐BB1340) was from Interchim FluoProbes.

Alcaraz L.B., Mallavialle A., Mollevi C., Boissière‐Michot F., Mansouri H., Simony‐Lafontaine J., Laurent‐Matha V., Chardès T., Jacot W., Turtoi A., Roger P., Guiu S, & Liaudet‐Coopman E. (2022). SPARC in cancer‐associated fibroblasts is an independent poor prognostic factor in non‐metastatic triple‐negative breast cancer and exhibits pro‐tumor activity. International Journal of Cancer, 152(6), 1243-1258.

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