Three dpf embryos were fixed in 2.5% paraformaldehyde, 5.0% glutaraldehyde, 0.06% picric acid in 0.2M cacodylate buffer overnight at 4°C. Samples were then embedded, cut into 50nm-thin sections, and stained with toluidine blue. Representative pictures were taken using a Nikon (Tokyo, Japan) Eclipse 50i, and the electron microscopy images were viewed with a JEOL (Tokyo, Japan) 1200EX–80kV.
1200ex 80kv
Manufactured by JEOL
Sourced in Japan
The 1200EX–80kV is a high-performance transmission electron microscope (TEM) manufactured by JEOL. It operates at an accelerating voltage of 80 kilovolts (kV) and is designed for advanced materials analysis and imaging applications.
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Lab products found in correlation
3 protocols using 1200ex 80kv
1
Ultrastructural Imaging of Embryonic Tissues
2
Characterization of Engineered Nanomaterials
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Differently surface coated Ag and TiO2 NMs were specifically selected, since both materials are routinely used in health and fitness products, and have the most potential for widespread environmental release [61 (link),62 (link)]. NMs used in this study include uncoated and PVP-coated TiO2 NMs (both supplied by Promethean Particles Ltd, Nottingham, UK), Ag2S PVP coated (AppNano Ltd., Barcelona, Spain), uncoated (bare) Ag NMs (Promethean Particles Ltd.) and PVP coated Ag NMs (Amepox Ltd., Łódź, Poland). A bulk Ag particle was also included (Sigma, Dorset, UK).
Using a Malvern Nanosizer 5000 instrument, dynamic light scattering (DLS) was used to measure the hydrodynamic size of the NMs. Transmission electron microscopy (TEM) was used to visualise the NMs, and analysis was performed using a JEOL 1200EX 80kv and a JEOL 1400Ex 80kv Max system. NM solutions were prepared for TEM by depositing 5–10 μL of the NM suspension onto a 300 mesh carbon-coated copper TEM grid (Agar Scientific, Stansted, UK) and allowing it to dry. Primary particle sizes were determined by counting at least 100 NMs.
Using a Malvern Nanosizer 5000 instrument, dynamic light scattering (DLS) was used to measure the hydrodynamic size of the NMs. Transmission electron microscopy (TEM) was used to visualise the NMs, and analysis was performed using a JEOL 1200EX 80kv and a JEOL 1400Ex 80kv Max system. NM solutions were prepared for TEM by depositing 5–10 μL of the NM suspension onto a 300 mesh carbon-coated copper TEM grid (Agar Scientific, Stansted, UK) and allowing it to dry. Primary particle sizes were determined by counting at least 100 NMs.
3
Mineralization Experiments: TEM Analysis
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Mineralization experiments
were prepared as above. After 0.5, 4, or 24 h, the reacting sample
was vortexed and 5 μL of solution was added to a Cu Formvar
TEM grid (Ted Pella) for 2 min. The solution was removed with filter
paper and dried in air before analysis with a JEOL 1200EX-80 KV instrument.
were prepared as above. After 0.5, 4, or 24 h, the reacting sample
was vortexed and 5 μL of solution was added to a Cu Formvar
TEM grid (Ted Pella) for 2 min. The solution was removed with filter
paper and dried in air before analysis with a JEOL 1200EX-80 KV instrument.
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