Mouse tnf α elisa kit

Manufactured by Cusabio

The Mouse TNF-α ELISA kit is a quantitative sandwich enzyme-linked immunosorbent assay (ELISA) designed for the detection and quantification of mouse tumor necrosis factor-alpha (TNF-α) in biological samples.

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Lab products found in correlation

Mouse il 6, by Cusabio (2 mentions) Mouse il 1β, by Cusabio (2 mentions) Mouse il 6 elisa kit, by Cusabio (2 mentions) Mouse il 6 elisa kit, by Thermo Fisher Scientific (1 mentions) Mouse inos elisa kit, by Abcam (1 mentions) Mouse il 6 elisa kit, by Abcam (1 mentions) Mouse ifn γ precoated elisa kit, by Dakewe (1 mentions) Mouse il 4 precoated elisa kit, by Dakewe (1 mentions) Mouse il 1β elisa kit, by Cusabio (1 mentions) Il 6 elisa kit, by Cusabio (1 mentions)

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TNF-α (46 citations) Mouse ELISA kits (17 citations) ELISAs (5 citations)

8 protocols using mouse tnf α elisa kit

Inflammatory Factors in Osteoarthritis

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The levels of the inflammatory factors IL-6, TNF-α, iNOS and COX2 in articular cartilage tissues of normal and OA mice were analysed using the corresponding kits,including the mouse IL-6 ELISA kit (BMS603-2; Invitrogen), mouse TNF-α ELISA kit (CSB-E04744m; Cusabio), mouse iNOS ELISA kit (ab253219; Abcam), and mouse COX2 ELISA kit (CSB-E12910m; Cusabio), according to the manufacturer’s instructions.

Li W., Tao C., Mao M, & Zhu K. (2023). The Nrf2/HMGB1/NF-κB axis modulates chondrocyte apoptosis and extracellular matrix degradation in osteoarthritis: Nrf2/HMGB1 affects osteoarthritis. Acta Biochimica et Biophysica Sinica, 55(5), 818-830.

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Quantifying Mouse Inflammatory Cytokines

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The mouse TNF‐α ELISA Kit (CSB‐E04741m, CUSABIO), mouse IL‐1β (CSB‐E08054m, CUSABIO), mouse IL‐10 (CSB‐E04634m, CUSABIO) and mouse IL‐6 (CSB‐E04639m) were used to determine the concentrations of TNF‐α, IL‐1β, IL‐6 and IL‐10 in plasma, respectively, following the manufacturer's instructions.

Ma Z., Wang W., Pan C., Fan C., Li Y., Wang W., Lan T., Gong F., Zhao C., Zhao Z., Yu S, & Yuan M. (2022). N‐acetylcysteine improves diabetic associated erectile dysfunction in streptozotocin‐induced diabetic mice by inhibiting oxidative stress. Journal of Cellular and Molecular Medicine, 26(12), 3527-3537.

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Cytokine Profiling in Mouse Samples

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The mouse TNF-α ELISA Kit (CSB-E04741m, CUSABIO), mouse IL-1β (CSB-E08054m, CUSABIO), mouse IL-4 (CSB-E04634m, CUSABIO), and mouse IL-6 (CSB-E04639m) were used to determine the concentrations of TNF-α, IL-1β, IL-6, and IL-4 in plasma or BALF, respectively following the manufacturer’s instructions.

Huang H., Chen M., Liu F., Wu H., Wang J., Chen J., Liu M, & Li X. (2019). N-acetylcysteine tiherapeutically protects against pulmonary fibrosis in a mouse model of silicosis. Bioscience Reports, 39(7), BSR20190681.

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Cytokine Measurement in Adipose Tissue

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The levels of TNF-α and IL-6 in cell culture medium and epididymal adipose tissues were determined by mouse TNF-α ELISA kit (CSB-E04741m, CUSABIO) and mouse IL-6 ELISA kits (ab100713, Abcam) according to the instructions of the manufacturers.

Tao J., Yu X.L., Yuan Y.J., Shen X., Liu J., Gu P.P., Wang Z., Ma Y.T, & Li G.Q. (2022). DMRT2 Interacts With FXR and Improves Insulin Resistance in Adipocytes and a Mouse Model. Frontiers in Endocrinology, 12, 723623.

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Quantifying Cytokine Levels in Mice

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According to the manufacturer’s instructions, ELISA was performed. The Mouse IL-6 ELISA KIT (CSB-E04639m) and Mouse TNF-α ELISA KIT (CSB-E04741m) were purchased from CUSABIO. The Mouse IL-10 ELISA kit (ELM-IL10-1) was purchased from RayBiotech Inc. (Peachtree Corners, GA, USA).

Jiang M., Qi Y., Huang W., Lin Y, & Li B. (2022). Curcumin Reprograms TAMs from a Protumor Phenotype towards an Antitumor Phenotype via Inhibiting MAO-A/STAT6 Pathway. Cells, 11(21), 3473.

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Cytokine Profiling of BMDM Supernatant

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The supernatant of BMDMs was analyzed by Mouse TGF‐β1 Precoated ELISA kit (DAKEWE, 1217102), Mouse IL‐4 Precoated ELISA kit (DAKEWE, 1210402), Mouse IFN‐γ Precoated ELISA kit (DAKEWE, 1210002), Mouse TNF‐α ELISA KIT (CUSABIO, CSB‐E04741m), Mouse VEGF ELISA Kit (CUSABIO, CSB‐E04756m), Mouse IL‐10 ELISA KIT (SenBeiJia Biological Technology Co., Ltd., SBJ‐M0073) according to the manufacturer's instructions.

Kong P., Liu X., Li Z., Wang J., Gao R., Feng S., Li H., Zhang F., Feng Z., Huang P., Wang S., Zhuang D., Ouyang W., Wang W, & Pan X. (2023). Biodegradable Cardiac Occluder with Surface Modification by Gelatin–Peptide Conjugate to Promote Endogenous Tissue Regeneration. Advanced Science, 11(2), 2305967.

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Cytokine Expression in Murine Colon

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The concentrations of NF-κB, IL-1β, and TNF-α in mouse colon tissue were determined using ELISA KIT. Mouse IL-1β ELISA KIT (CSB-E08054m) and Mouse TNF-α ELISA KIT (CSB-E04741m) were purchased from CUSABIO (Wuhan, China). Mouse NF-κB ELISA KIT (ml058779) was obtained from Enzyme-linked Biotechnology Co., Ltd (Shanghai, China). All steps were carried out in strict accordance with the instructions. The absorbance of each well at 450 nm was measured by a microplate reader (MB-530, HEALES, China) within 5 min after the termination of the reaction.

Rong Z.J., Cai H.H., Wang H., Liu G.H., Zhang Z.W., Chen M, & Huang Y.L. (2022). Ursolic Acid Ameliorates Spinal Cord Injury in Mice by Regulating Gut Microbiota and Metabolic Changes. Frontiers in Cellular Neuroscience, 16, 872935.

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Quantification of Cytokine Secretion

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The supernatants were collected from cell cultures at the indicated time, and the concentrations of TNF-α and IL-6 were assayed using TNF-α ELISA kit (cat. no. CSB-E04741m; Mouse TNF-α ELISA kit; Cusabio Biotech) and IL-6 ELISA kit (cat. no. CSB-E04639m; Mouse IL-6 ELISA kit; Cusabio Biotech), according to the manufacturer's instructions.

Zheng X., Zhong Q., Lin X., Gu X., Ling X., Liang Z., Qin Q, & Du X. (2021). Transforming growth factor-β1-induced podocyte injury is associated with increased microRNA-155 expression, enhanced inflammatory responses and MAPK pathway activation. Experimental and Therapeutic Medicine, 21(6), 620.

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