Nchr probe

Aqueous samples (5 μL of concentration 2.5 μL/mL) were sonicated, drop deposited on freshly cleaved mica and dried over in vacuum at 30 °C for 20 min. The samples were maintained in desiccator until analysis. Topography images were obtained in a NX-10 Atomic Force Microscope (Park Systems, Suwon, South Korea) in an acrylic glove box with controlled temperature (around 22 °C) and humidity (around 3%). AFM imaging was acquired at tapping mode using a NCHR probe (NanoWorld) with a spring constant of 42 N/m and 320 kHz resonance frequency. The imaging was obtained with a scan speed of 0.5 Hz with a scanning resolution of 512 × 512 points. For each sample, at least 10 images were collected. Image measurements and automatic processing (plane subtraction and rows alignment) were performed using Gwyddion 2.47 software (http://gwyddion.net/).

Un-1-WSF and R-2-WSF were diluted in water and sonicated (2.5 µg/mL). The samples were dropped onto freshly cleaved mica, dried in a vacuum at 30 °C for 20 min and maintained in a desiccator until the analysis^{20 (link)}. An NX-10 AFM (Park Systems, Suwon, South Korea) in an acrylic glove box was used to obtain topography images with controlled temperature (~22 °C) and humidity (~3%). AFM images were acquired on tapping mode using an NCHR probe (NanoWorld) with a spring constant of 42 N/m and 320 kHz resonance frequency. The scan speed and scanning resolution were 0.5 Hz and 512 × 512 points, respectively. At least ten images were collected for each sample. Gwyddion 2.47 software (http://gwyddion.net/) was used for image measurements and automatic processing (plane subtraction and row alignment)^{20 (link)}.