Chromeleon computer system

HPLC with electrochemical detection was used to measure striatal levels of dopamine, DOPAC and serotonin using a method that has been described (Sathe et al., 2012 (link), Nuber et al., 2008 (link)). Briefly, after mice were killed, striata were dissected out and snap frozen directly on dry ice. Striata were then homogenized in 1% perchloric acid (PCA) (1:30 [i.e. tissue weight: PCA volume]) and centrifuged at 14,000g at 4 °C for 20 min. Following centrifugation, 20 μl of the supernatant sample was injected onto a C18 column (Dionex, Germering, Germany). The mobile phase consisted of 90% 50 mM sodium acetate, 35 mM citric acid, 105 mg/L octane sulfonic acid, 48 mg/L sodium EDTA solution, and 10% methanol at pH 4.3. Flow rate was 1 ml/min. Peaks were detected by an ESA Coulochem II electrochemical detector (ESA), and the detector potential was set at 700 mV. Data were collected and processed using the Chromeleon computer system (Dionex).

High‐performance liquid chromatography (HPLC) with electrochemical detection was used to measure striatal levels of dopamine, and DOPAC using a method that has been described (Sathe et al., 2012). Briefly, mice were killed, 21 days after the last MPTP injection, and the striata were dissected out and snap frozen on solid carbon dioxide. Striata were then homogenised in 0.1 M perchloric acid (1:30 wt/vol), sonicated and centrifuged at 18,600 x g at 4°C for 20 mins. Following centrifugation 20 μl of sample was injected onto a C18 column (Dionex, Germering, Germany) The mobile phase consisted of 90% 50 mM sodium acetate, 35 mM citric acid, 105 mg/L octane sulfonic acid, 48 mg/L sodium EDTA solution, and 10% methanol at pH 4.3. Flow rate was 1 ml/min. Peaks were detected by an ESA Coulochem II electrochemical detector (ESA, Dionex), and the detector potential was set at 700 mV. Data were collected and processed using the Chromeleon computer system (Dionex).