Ab211651

ELISA kits for Insulin-like growth factor I (IGF1, #ab211651, intra-assay coefficient of variation (CV) 2.3%) and Insulin-like growth factor-binding protein 3 (IGFBP3, #ab211652, intra-assay CV 3.1%) were purchased from Abcam (Cambridge, MA), and ELISA kit for Insulin-like growth factor-binding protein 2 (IGFBP2, #DGB200, intra-assay CV 5%) was from R&D Systems (Minneapolis, MN), and ELISA kit for Anti-mullerian hormone (AMH, #AL-124, intra-assay CV 3.7%) was purchased from AnshLabs (Webster, TX). The plasma samples from each time point of selected subjects were diluted with dilution factors of 1:80, 1:100, and 1:2000 for IGF1, IGFBP2, and IGFBP3 detection, respectively; and for AMH measurement, dilution factors of 1:10 and 1:500 were used for samples from females and males, respectively. All ELISA measurements were conducted according to manufacturer’s protocol. Duplicated wells were used to average the absorbance values and the concentration of target proteins in the plasma were calculated based on the calibration curve (R² = 0.9848, 0.9999, 0.9936 and 0.9971 for IGF1, IGFBP2, IGFBP3, and AMH, respectively.) and dilution factor after subtraction of background.