A total of 24 C57BL/6N mice were randomly assigned to three groups, including Ctrl., Model-N.S., and Model-10b -100 mg·kg−1, with eight animals per group. The randomization process was performed using Research Randomizer (RRID: SCR_008563). CDHFD (D09100310) diet was obtained from Research Diets Inc. Sixteen mice were fed the CDHFD diet for 12 weeks to induce NASH, with eight mice receiving a normal diet as a control. At the beginning of the 13th week, the mice in the model group were randomly assigned into two groups and given daily gavages of either normal saline (Model-N.S. group) or 100 mg·kg−1 of compound 10b suspended in 0.5% sodium carboxymethylcellulose solution (Model-10b groups) for four weeks. Daily bodyweights were recorded, and blood samples were collected from the abdominal aorta following an overnight fast. Afterward, the mice were euthanized using isoflurane. Liver tissue samples were obtained, and some were treated with 4% paraformaldehyde to prepare them for future histological analysis, while others were kept at −80 °C for later examination. Maximum liver lobule was collected for each mouse.
Cd hfd
Manufactured by Research Diets
Sourced in China
The CD-HFD is a laboratory equipment product designed for research purposes. It serves as a tool for controlled diet preparation, specifically for high-fat diet studies. The core function of the CD-HFD is to facilitate the precise formulation and delivery of high-fat diets for experimental research.
Automatically generated - may contain errors
Lab products found in correlation
D12492, by Research Diets (3 mentions)
High fat diet (hfd), by Research Diets (2 mentions)
Trifast reagent, by Avantor (1 mentions)
P1324, by Altromin (1 mentions)
C57bl 6 mice, by Research Diets (1 mentions)
C57bl 6 mice, by Jackson ImmunoResearch (1 mentions)
D12451, by Research Diets (1 mentions)
Mcd diet, by Research Diets (1 mentions)
D05010402, by Research Diets (1 mentions)
Td 88137, by Inotiv (1 mentions)
5 protocols using cd hfd
1
Evaluation of Compound 10b in NASH
2
Choline Deficient High-Fat Diet in Cyp7b1-Deficient Mice
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All mouse experiments were approved by the Animal Welfare Officers of University Medical Centre Hamburg-Eppendorf (UKE) and Behörde für Gesundheit und Verbraucherschutz Hamburg (G96/15). Cyp7b1−/− mice were bred and housed in the animal facility of the UKE at 22 °C under a day-night cycle of 12 h and ad libitum access to drinking water and regular chow diet (P1324, Altromin, Germany). For our study, male age-matched Cyp7b1−/− and WT littermates (11–12 weeks-old in the beginning of the experiment) were fed for a period of 8 months with a choline deficient high fat diet (CD-HFD, Research Diets; D05010402). At the end of the study, EDTA blood was harvested after a 4 h fasting period. Subsequently, animals were perfused with PBS and tissues were either conserved in TriFast reagent (Peqlab, Erlangen, Germany) for RNA analysis, fixed in 3.7% formaldehyde solution for histology or snap frozen and stored at −80 °C for lipid and protein analysis. Body composition analysis was performed 1 week before the end of the experiment by echoMRI.
3
Dietary Interventions and Rapamycin in Mice
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Eight-week-old weight-matched male C57BL/6 mice and CD45.1 congenic C57BL/6 mice were purchased from The Jackson Laboratory (ME, USA). The C57BL/6 mice were fed either a normal control diet (NCD), a HFD (D12492, Research Diets, NJ, USA), a MCD (10401, Beijing HFK Bioscience, Beijing, China), or a CD-HFD (Research Diets, D05010402). C57BL/6 mice fed with MCD and treated with rapamycin (0.5 mg/kg/day, intraperitoneally) as a positive control for 4 weeks. The mice were maintained in a pathogen-free, temperature-controlled environment with a 12-h light/dark cycle. The animal studies were performed in compliance with the ethical guidelines for animal studies and approved by the Institutional Animal Care and Ethics Committee of Beijing Friendship Hospital.
4
High-fat Diet Mouse Models for Obesity
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Animal experiments were conducted in accordance to the standards approved by the Faculty Animal Committee at the University of Santiago de Compostela, and the experiments were performed in agreement with the Rules of Laboratory Animal Care and International Law on Animal Experimentation. 8–10 weeks old Swiss mice were kept under a 12 h light/dark cycle and had ad libitum access to chow diet and water. In the first study, we used Swiss mice (8–10 weeks old) kept under a 12 h light/dark cycle and with ad libitum access to chow diet and water mice fed a HFD (Research Diets D12451; 45% fat, 4.73 kcal/g) for 3 months before undergoing the treatments. In a second study, we used C57BL/6J mice (8–10 weeks old) fed a HFD (Research Diets, D12492; 60% fat, 5.24 kcal/g) for 2 months, MCD diet (D05010402, Research Diets) for 2 weeks and CD-HFD (A0282002B, Research diets) for 4 weeks before undergoing the treatments. p53 floxed mice were purchased from The Jackson Laboratory (B6.129P2-Trp53tm1Brn/J) and maintained on a Specific Pathogen Free room (SPF) and fed with chow diet or HFD (Research Diets, D12492; 60% fat, 5.24 kcal/g) for two months before undergoing the treatments. Food intake and body weight were measured twice a week during experimental phase in all experiments.
5
Dietary Interventions in Mice Model
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In vivo studies were performed in accordance with European guidelines for the use and care of laboratory animals and approved by an independent ethics committee (authorization 17430-2018110611093660 v3) .
Thirteen-week-old male and female C57BL/6J mice (n=12 per group and per sex) were fed a chow diet (CTRL, D12450J, Research Diets), a high-fat diet (HFD, D12492, Research Diets), choline-deficient high-fat diet (CDHFD, D05010402, Research Diets), Western diet (WD, TD.88137, Envigo), or WD with glucose (18.9 g/L) and fructose (23.1 g/L) in drinking water (WD GF) [24] (link) for 15 weeks.
Thirteen-week-old male and female C57BL/6J mice (n=12 per group and per sex) were fed a chow diet (CTRL, D12450J, Research Diets), a high-fat diet (HFD, D12492, Research Diets), choline-deficient high-fat diet (CDHFD, D05010402, Research Diets), Western diet (WD, TD.88137, Envigo), or WD with glucose (18.9 g/L) and fructose (23.1 g/L) in drinking water (WD GF) [24] (link) for 15 weeks.
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