The rats (n = 4 per group) without undergoing MRI experiments were deeply anesthetized. The perilesional cortex was separated, and protein levels were determined by Western blotting, as previously described (Zhan et al., 2020 (link)). Proteins were transferred onto polyvinylidene difluoride membranes, followed by blocking them with 5% nonfat milk for 2 h and subsequently incubating membranes at 4°C overnight with primary antibodies: anti-GAP-43 (1:40000; Epitomics, #2259-1), SYN (1:320000; Epitomics, #1870-1), Netrin-1 (1:2,000; Abcam, ab126729), DCC (1:1,000; Abcam, ab125280), Slit-2 (1:10,000; Abcam, ab134166), Robo-1 (1:1,000; Abcam, ab7279), NogoA (1:20,000; Abcam, ab62024), NgR (1:40,000; Abcam, ab62024), RhoA (1:20,000; Cell signaling, 2117s), ROCK-2 (1:50,000; Abcam, ab125025), and GAPDH (1:1,60,000; GeneTex, GTX627408). After washing, membranes were incubated with secondary anti-rabbit (1:20,000; Applygen Technologies Inc., C1309) or anti-mouse (1:20,000; NeoBioscience, cat. ANM 02-1, Lot. 0912) IgG (H + L)-HRP for 1 h at room temperature. Immunoreactive protein bands were detected by using the SuperECL Plus kit (Applygen, China, cat. No. P1050) and chemiluminescent imager (VILBER, United States). The intensities of target proteins were quantified by ImageJ software.
Ab134166
Manufactured by Abcam
Sourced in United Kingdom
Ab134166 is a monoclonal antibody that can be used for the detection of a specific target protein in various applications such as Western blotting, ELISA, and immunohistochemistry. The core function of this antibody is to specifically bind to and detect the target protein in biological samples.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using ab134166
1
Protein Expression Analysis in Rat Cortex
2
Quantifying Slit2 Expression in Lung Tissue
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The right lungs were fixed in 10% formalin for 24 h before embedding in paraffin; midsagittal sections were stained with hematoxylin and eosin (HE) for histological analysis, we determined enlargement of alveolar spaces by quantifying the Lm as previously described.11 (link) Lung tissue sections were deparaffinized with xylene, hydrated with alcohol, repaired with sodium citrate in high temperature and then incubated with antibody against mouse Slit2 (ab134166; Abcam, Cambridge, UK) overnight at 4 °C. Subsequently, secondary Goat Anti-Rabbit IgG H&L (HRP) antibody (ab205718, Abcam, Cambridge, UK) was stained at room temperature for 20 mins and finally reacted with diaminobenzidine (Solarbio, DA1010, China) for coloration. Fluorescence pathological microscope (Olympus */BX53+DP80, Japan) was used to take photos for all the sections. The mean density of SLIT2 staining was quantified using Image-Pro Plus software 6.0 (Media Cybernetics), and the average score of 2 different viewers was taken for analysis.
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