Polychromatic Flow Cytometry involved 1–5 × 105 cells being incubated with respective antibodies for 30 min/4 °C and LIVE/DEAD® Fixable Near-IR or Blue fluorescent Dead Cell Stain Kit, washed, optionally incubated with secondary antibodies and acquired at LSRII (BD Biosciences). In tissue-cell-suspensions, CD45 was used to identify leukocytes after exclusion of dead cells and doublets. Myeloid cells were selected based on CD11c (pan-myeloid marker in human) with exclusion of CD3+ T cells. Within CD11c+ cells, ercDCs and macrophages were distinguished as CD209+CD14+ double-positive cells (ercDCs) and CD14 single-positive cells (macrophages). For M1/M2-macrophages, live cells where selected and doublets were excluded before marker analysis. Antibodies are listed in Table S3 .
Live dead fixable near ir or blue fluorescent dead cell stain kit
Manufactured by BD
The LIVE/DEAD® Fixable Near-IR or Blue fluorescent Dead Cell Stain Kit is a reagent that can be used to label and identify dead cells in a sample. The kit provides a fluorescent dye that selectively binds to proteins in dead cells, allowing them to be distinguished from live cells using flow cytometry or other fluorescence-based analysis methods.
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Lab products found in correlation
2 protocols using live dead fixable near ir or blue fluorescent dead cell stain kit
1
Polychromatic Flow Cytometry for Immune Cell Profiling
2
Comprehensive Myeloid Cell Profiling
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1-5 x 10 5 cells were incubated with antibodies for 30 min/4°C and LIVE/DEAD® Fixable Near-IR or Blue fluorescent Dead Cell Stain Kit, washed, optionally incubated with secondary antibodies and acquired at LSRII (BD Biosciences). In tissue-cellsuspensions, CD45 was used to identify leukocytes after exclusion of dead cells and doublets. Myeloid cells were selected based on CD11c (pan-myeloid marker in human)
with exclusion of CD3 + T cells. Within CD11c + cells, ercDCs and macrophages were distinguished as CD209/CD14 double-positive cells (ercDCs) and CD14 singlepositive cells (macrophages). For M1/M2-macrophages, live cells where selected and doublets excluded before marker analysis. Antibodies are listed in table S13.
with exclusion of CD3 + T cells. Within CD11c + cells, ercDCs and macrophages were distinguished as CD209/CD14 double-positive cells (ercDCs) and CD14 singlepositive cells (macrophages). For M1/M2-macrophages, live cells where selected and doublets excluded before marker analysis. Antibodies are listed in table S13.
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