Carbon coated copper formvar grids

Manufactured by Agar Scientific

Sourced in United Kingdom

Carbon-coated copper formvar grids are a type of specimen support used in transmission electron microscopy (TEM). They consist of a copper mesh grid coated with a thin layer of formvar polymer, which is then further coated with a thin layer of carbon. This configuration provides a stable and conductive support for samples to be examined under the electron beam of a TEM.

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Lab products found in correlation

Jem 1400, by JEOL (5 mentions)

Close spelling variants of this product

Formvar carbon-coated copper grids 300 mesh carbon/formvar coated copper grid Formvar carbon-coated 300-mesh copper electron microscopy grids Formvar and carbon-coated copper grids Formvar/carbon-coated 400-mesh copper grids Formvar/carbon-coated 300-mesh copper grids Formvar/carbon-coated and glow-discharged copper EM grids Carbon–formvar-coated copper EM grids Formvar/carbon coated 400 mesh copper TEM support grids Formvar Carbon coated 200 mesh copper grids

5 protocols using carbon coated copper formvar grids

Transmission Electron Microscope Imaging

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EV imaging was conducted via a JEOL JEM1400 transmission electron microscope (TEM) coupled with an AMT XR80 digital acquisition system. Samples were physisorbed to 200 mesh carbon‐coated copper formvar grids (Agar Scientific, UK) and negatively stained with 1% uranyl acetate.

Man K., Brunet M.Y., Fernandez‐Rhodes M., Williams S., Heaney L.M., Gethings L.A., Federici A., Davies O.G., Hoey D, & Cox S.C. (2021). Epigenetic reprogramming enhances the therapeutic efficacy of osteoblast‐derived extracellular vesicles to promote human bone marrow stem cell osteogenic differentiation. Journal of Extracellular Vesicles, 10(9), e12118.

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Transmission Electron Microscopy of EVs

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The morphology of isolated EVs was conducted via a JEOL JEM1400 transmission electron microscope (TEM) coupled with an AMT XR80 digital acquisition system. Samples were physiosorbed to 200 mesh carbon‐coated copper formvar grids (Agar Scientific, United Kingdom) and negatively stained with 1% uranyl acetate.

Man K., Brunet M.Y., Federici A.S., Hoey D.A, & Cox S.C. (2022). An ECM-Mimetic Hydrogel to Promote the Therapeutic Efficacy of Osteoblast-Derived Extracellular Vesicles for Bone Regeneration. Frontiers in Bioengineering and Biotechnology, 10, 829969.

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Transmission Electron Microscopy of EVs

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EV imaging was conducted via a JEOL JEM1400 transmission electron microscope (TEM) coupled with an AMT XR80 digital acquisition system. Samples were physisorbed to 200 mesh carbon-coated copper formvar grids (Agar Scientific, Stansted, UK) and negatively stained with 1% uranyl acetate.

Man K., Barroso I.A., Brunet M.Y., Peacock B., Federici A.S., Hoey D.A, & Cox S.C. (2022). Controlled Release of Epigenetically-Enhanced Extracellular Vesicles from a GelMA/Nanoclay Composite Hydrogel to Promote Bone Repair. International Journal of Molecular Sciences, 23(2), 832.

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TEM Imaging of Extracellular Vesicles

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EVs were imaged using the JEOL JEM1400 transmission electron microscope coupled with an AMT XR80 digital acquisition system. The vesicles were physisorbed to 200-mesh carbon-coated copper formvar grids (Agar Scientific, Stansted, UK) and negatively stained with 1% uranyl acetate.

Man K., Brunet M.Y., Lees R., Peacock B, & Cox S.C. (2023). Epigenetic Reprogramming via Synergistic Hypomethylation and Hypoxia Enhances the Therapeutic Efficacy of Mesenchymal Stem Cell Extracellular Vesicles for Bone Repair. International Journal of Molecular Sciences, 24(8), 7564.

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Transmission Electron Microscopy of Microvesicles

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Imaging of MVs was conducted via a JEOL JEM1400 transmission electron microscope (TEM) coupled with an AMT XR80 digital acquisition system. MVs were physisorbed onto 200 mesh carbon-coated copper formvar grids (Agar Scientific, UK) and negatively stained with 1% uranyl acetate.

Luo L., Foster N.C., Man K.L., Brunet M., Hoey D.A., Cox S.C., Kimber S.J, & El Haj A.J. (2022). Hydrostatic pressure promotes chondrogenic differentiation and microvesicle release from human embryonic and bone marrow stem cells. Biotechnology journal, 17(4).

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