3d gene human mirna oligo chips mir base

RNA extraction was performed as described previously.³¹ (link) For miR expression profiling, 3D-Gene Human miRNA Oligo Chips (miR Base, version 17-19; Toray Industries, Tokyo, Japan) were used and analyzed as reported previously.³¹ (link) All data were globally normalized per microarray such that the median of the signal intensity was adjusted to 25 (Fig. 3).

RNA extraction was performed as previously described.⁶ (link) For miR expression profiling, 3D-Gene Human miRNA Oligo Chips (miRBase, version 17-19; Toray Industries) were used and analyzed as previously reported.⁶ (link) All data were globally normalized per microarray such that the median of the signal intensity was adjusted to 25. Cellular miR expression profiles were distinct among hCEC subpopulations (SPs); they showed heterogeneous expression levels of CD44. Using these hCEC SPs, miRs exhibiting distinct intracellular expression levels were selected using volcano plots, in which the x- and y-axes indicated the log2 value of the expression fold change (abundance ratio) and the negative log10 value of P values, respectively.