For determination of energy expenditure, respiratory exchange ratios, and activity, mice were placed in an Oxymax metabolic chamber system (Comprehensive Laboratory Animal Monitoring System, CLAMS, from Columbus Instruments (Columbus, OH)). Oxygen consumption (V̇O2), carbon dioxide production (V̇CO2), and ambulatory activity were determined for each mouse over 72 hours Only the last two complete light and dark cycles (spanning 48 hours) were used for data analysis. Feces were collected during the metabolic cage studies and shipped to the Mouse Metabolic Phenotyping Core at the University of Texas Southwestern for bomb calorimetry analysis using a Parr 6200 Isoperibol Calorimeter.
Oxymax metabolic chamber system
Manufactured by Columbus Instruments
Sourced in United States
The Oxymax metabolic chamber system is a laboratory equipment designed for the measurement of metabolism. It provides data on oxygen consumption and carbon dioxide production in a controlled environment.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using oxymax metabolic chamber system
1
Metabolic Profiling of Mice
2
Metabolic Phenotyping of Mice
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Food intake was measured for mice placed in individual cages during at least 3 consecutive days. Lean mass, fat mass, and percent fat were determined on awake mice using an Echo MRI analyzer (Whole Body Composition Analyzer, Echo medical systems, Houston, TX, USA). Females underwent a glucose tolerance test (GTT) after an overnight fast. Glucose (2 mg/g b.w.) was administered intraperitoneally, and glucose levels were determined in tail blood using an ACCU‐CHEK® Aviva blood glucose monitor and appropriate test strip (Roche Diagnostics). Oxygen consumption (VO2), carbon dioxide production (VCO2), and spontaneous locomotor activity were determined using indirect, open‐circuit calorimetry in an Oxymax Metabolic Chamber system (Columbus Instruments, Columbus, OH, USA). The mice (with free access to food and water) were housed in separate chambers for 48 h for acclimatization before starting 48 h measurements. The average VO2 was calculated during 48 h—two light cycles and two dark cycles (Somm et al, 2005 , 2014 ).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!