Mouse hmgb 1 elisa kit

Manufactured by Elabscience

Sourced in United States, China

The Mouse HMGB-1 ELISA Kit is a laboratory equipment product designed to quantify the levels of High Mobility Group Box 1 (HMGB-1) protein in mouse samples using the enzyme-linked immunosorbent assay (ELISA) technique.

Automatically generated - may contain errors

Lab products found in correlation

Human hmgb1 elisa kit, by Elabscience (2 mentions) Mouse ccl2 je mcp 1 quantikine elisa kit, by R&D Systems (2 mentions) Tnfα mouse high sensitivity elisa kit, by Thermo Fisher Scientific (2 mentions) Il6 mouse high sensitivity elisa kit, by Thermo Fisher Scientific (2 mentions) Atp determination kit, by Thermo Fisher Scientific (1 mentions) Mouse il 18 elisa kit, by Abcam (1 mentions) Mouse s100a8 elisa kit, by Abcam (1 mentions) Human il 1β elisa kit, by Abcam (1 mentions) Mouse il 1β elisa kit, by Abcam (1 mentions) Hsp70 elisa kit, by Abcam (1 mentions) Mouse s100a9 elisa kit, by Abcam (1 mentions) Human il 18 elisa kit, by Abcam (1 mentions) Atp assay kit, by Beyotime (1 mentions) Afp quantikine elisa kit, by R&D Systems (1 mentions) Mouse alpha fetoprotein afp quantikine elisa kit, by R&D Systems (1 mentions) Mouse tnf α elisa kit, by Elabscience (1 mentions) Human il 6 elisa kit, by Neobioscience (1 mentions) Mouse il 6 elisa kit, by Elabscience (1 mentions) Human tnf α elisa kit, by Neobioscience (1 mentions)

6 protocols using mouse hmgb 1 elisa kit

Extracellular ATP and HMGB1 Quantification

Check if the same lab product or an alternative is used in the 5 most similar protocols

CT26 cells were seeded in 6-well plates (2 × 10⁵ cells/well) and incubated with 1.0 mL of complete medium for 24 h. The cells were then treated with NP/mDs and each component for another 24 h. Afterward, the cell supernatant was collected and extracellular ATP and HMGB1 concentrations were determined by ATP Determination Kit (A22066, Thermo Fisher Scientific, USA) and Mouse HMGB-1 ELISA Kit (E-EL-M0676c, Elabscience Biotechnology, China), respectively, according to the manufacturer protocols with a Hybrid Multi-Mode Reader.

Chen X., Ling X., Xia J., Zhu Y., Zhang L., He Y., Wang A., Gu G., Yin B, & Wang J. (2021). Mature dendritic cell-derived dendrosomes swallow oxaliplatin-loaded nanoparticles to boost immunogenic chemotherapy and tumor antigen-specific immunotherapy. Bioactive Materials, 15, 15-28.

+ Open protocol

+ Expand

HMGB1 Quantification by ELISA

Check if the same lab product or an alternative is used in the 5 most similar protocols

Serum samples and cell culture supernatants were collected and used for assessing HMGB1 level by enzyme‐linked immunosorbent assay (ELISA), according to the manufacturer's instructions for human HMGB1 ELISA kit (Elabscience) and mouse HMGB1 ELISA kit (Elabscience).

Le Y., Wang Y., Zhou L., Xiong J., Tian J., Yang X., Gai X, & Sun Y. (2019). Cigarette smoke‐induced HMGB1 translocation and release contribute to migration and NF‐κB activation through inducing autophagy in lung macrophages. Journal of Cellular and Molecular Medicine, 24(2), 1319-1331.

+ Open protocol

+ Expand

Quantification of DAMP Markers in LPS-Stimulated Cell Cultures

Check if the same lab product or an alternative is used in the 5 most similar protocols

Human IL-18 ELISA Kit (ab215539), Mouse IL-18 ELISA Kit (ab216165), Human IL-1β ELISA Kit (ab214025), Mouse IL-1β ELISA Kit (ab197742), Mouse S100A8 ELISA Kit (ab263886), Mouse S100A9 ELISA Kit (ab213887) and HSP70 ELISA Kit (ab133060) were purchased from Abcam, UK. Human S100A8 (E-EL-H1289c), Human S100A9 (E-EL-H1290c), HSP90 ELISA Kit (E-EL-H1864c), Human HMGB-1 ELISA Kit (E-EL-H1554c), Mouse HMGB-1 ELISA Kit (E-EL-M0676c) were obtained from Elabscience Biotechnology Co., Ltd, China. ATP Assay Kit (S0027) was purchased from Beyotime, China.
Briefly, differentiated U937 or RAW264.7 was stimulated with LPS for 6 h. Next, TDCA was added to the medium at the final concentration of 200 μM for 24 h. Subsequently, the supernatant was collected to measure the level of DAMPs including HSP70, HSP90, S100A8, S100A9, and ATP. All assays were performed following the instructions of the producer.

Zhao C., Mu M., Li X., Dong Z., Wang J., Yao C., Zheng J., Sun X, & Yu J. (2024). USP50 regulates NLRP3 inflammasome activation in duodenogastric reflux-induced gastric tumorigenesis. Frontiers in Immunology, 15, 1326137.

+ Open protocol

+ Expand

Cytokine and Biomarker Quantification

Check if the same lab product or an alternative is used in the 5 most similar protocols

The following ELISA kits were used to determine plasma levels of the following as per manufacturer's instructions. TNFα mouse high sensitivity ELISA kit (#BMS607-3) and IL6 mouse high sensitivity ELISA kit (#BMS603HS; Thermo Fisher Scientific), mouse CCL2/JE/MCP-1 quantikine ELISA kit (#MJE00B), mouse alpha-fetoprotein (AFP) quantikine ELISA kit (#MAFP00; R&D Systems), mouse HMGB1 ELISA Kit (#E-EL-M0676, Elabscience).

Mohammed S., Thadathil N., Ohene-Marfo P., Tran A.L., Van Der Veldt M., Georgescu C., Oh S., Nicklas E.H., Wang D., Haritha N.H., Luo W., Janknecht R., Miller B.F., Wren J.D., Freeman W.M, & Deepa S.S. (2023). Absence of Either Ripk3 or Mlkl Reduces Incidence of Hepatocellular Carcinoma Independent of Liver Fibrosis. Molecular Cancer Research, 21(9), 933-946.

+ Open protocol

+ Expand

Mouse Cytokine and Protein ELISA Assays

Check if the same lab product or an alternative is used in the 5 most similar protocols

The following ELISA kits were used to determine plasma levels of the following as per manufacturer’s instructions. TNFα mouse high sensitivity ELISA kit (# BMS607-3)and IL-6 mouse high sensitivity ELISA kit (# BMS603HS) (Thermo Fisher Scientific), mouse CCL2/JE/MCP-1 quantikine ELISA kit (# MJE00B), mouse alpha-fetoprotein/AFP quantikine ELISA kit (#MAFP00) (R&D Systems), mouse HMGB1 ELISA Kit (#E-EL-M0676, Elabscience, Houston, TX).

+ Open protocol

+ Expand

Quantifying Cytokine Levels in Melanoma Cell Cultures and Mouse Serum

Check if the same lab product or an alternative is used in the 5 most similar protocols

ELISA analysis on culture medium of melanoma cells or the blood serum of mice after indicated treatment was performed using the Human IL-6 ELISA Kit (Neobioscience, EHC007.96), Human TNF-α ELISA Kit (Neobioscience, EHC103a.96), Human MIG/CXCL9 ELISA Kit (Neobioscience, EHC114.96), Human IP-10/CXCL10 ELISA Kit (Neobioscience, EHC157.96), Human I-TAC/CXCL11 ELISA Kit (Neobioscience, EHC084.96), Mouse TNF-α ELISA Kit (Elabscience, E-MSEL-M0002), Mouse IL-2 ELISA Kit (Elabscience, E-MSEL-M0036), Mouse IL-6 ELISA Kit (Elabscience, E-EL-M0044c), Mouse ELISA Kit MIG/CXCL9 ELISA Kit (Elabscience, E-EL-M3077), Mouse IP-10/CXCL10 ELISA Kit (Elabscience, E-EL-M0021c), Mouse I-TAC/CXCL11 ELISA Kit (Elabscience, E-EL-M0056c) and Mouse HMGB-1 ELISA Kit (Elabscience, E-EL-M0676c) according to the manufacturer’s instructions. The absorbance (A450 nm) was measured with a plate reader (Bio-Rad). The ELISA result was normalized by cell counting of the attached live cells after harvesting the supernatants.

Yang Y., Wang S., Wang X.X., Guo S., Wang H., Shi Q., Tian Y., Wang H., Zhao T., Zhang H., Zhang B., Gao T., Li C., Yi X, & Guo W. (2024). Tumorous IRE1α facilitates CD8+T cells-dependent anti-tumor immunity and improves immunotherapy efficacy in melanoma. Cell Communication and Signaling : CCS, 22, 83.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!