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Human igg igm elisa quantitation set

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The Human IgG/IgM ELISA Quantitation Set is a laboratory tool used for the quantitative determination of human immunoglobulin G (IgG) and immunoglobulin M (IgM) levels in biological samples. It provides a standardized method for measuring these important antibody classes.

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Human IgM ELISA Quantitation Set ELISA Quantitation Set Human IgM

3 protocols using human igg igm elisa quantitation set

1

Serum Immunoglobulin and Anti-EBV Antibody Levels

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Serum immunoglobulin (Igs) and anti-EBV Ab concentrations were measured by ELISA. The total IgG and IgM concentrations of 38 patients and 11 healthy controls were measured using a Human IgG/IgM ELISA Quantitation Set (Bethyl Laboratories, Montgomery, TX). The anti-EBV Ab concentrations of all subjects, comprising EBV-EA-IgG and EBV-VCA-IgG, were measured by ELISA (EA; Vircell, Granada, Spain, VCA; Abcam, Cambridge, United Kingdom) according to the manufacturer's instructions. Serum levels of total IgG/IgM, EBV-EA-IgG, and EBV-VCA-IgG are shown in Table 1.
Kumata K., Nagata K., Matsushita M., Kuwamoto S., Kato M., Murakami I., Fukata S, & Hayashi K. (2016). Thyrotropin Receptor Antibody (TRAb)-IgM Levels Are Markedly Higher Than TRAb-IgG Levels in Graves' Disease Patients and Controls, and TRAb-IgM Production Is Related to Epstein–Barr Virus Reactivation. Viral Immunology, 29(8), 459-463.
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2

Quantification of IgG and IgM

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Supernatants from co-cultured cells were collected. Total IgG/M were detected using human IgG/IgM ELISA quantitation set (Bethyl Laboratories) according to the manufacturer’s instructions.
Kam N.W., Wu K.C., Dai W., Wang Y., Yan L.Y., Shakya R., Khanna R., Qin Y., Law S., Lo A.W., Lee V.H., Guan X.Y, & Kwong D.L. (2021). Peritumoral B cells drive proangiogenic responses in HMGB1-enriched esophageal squamous cell carcinoma. Angiogenesis, 25(2), 181-203.
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3

ELISA Quantification of IgG and IgM

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Immunoglobulin (Ig) concentrations in culture supernatant were measured by ELISA. The total IgG and IgM concentrations were measured using a Human IgG/IgM ELISA Quantitation Set (Bethyl Laboratories, Montgomery, TX).
Hara S., Nagata K., Kumata K., Matsushita M., Kuwamoto S., Kato M, & Hayashi K. (2018). Estradiol Affects Epstein–Barr Virus Reactivation-Induced Thyrotropin Receptor Antibody and Immunoglobulin Production in Graves’ Disease Patients and Healthy Controls. Viral Immunology, 31(7), 486-491.
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