Rat adrenal pheochromocytoma cell lines (PC12 cells) were obtained from the Shanghai Institute of Cell Biology (Shanghai, China). Fetal bovine serum (FBS), penicillin/streptomycin, and Dulbecco’s modified Eagle’s medium (DMEM) were purchased from Gibco Life Technologies (Rockville, MD, US). Cell counting kit-8 (CCK-8) was purchased from Dojindo Laboratories (Tokyo, Japan). The In Situ Cell Death Detection Kit was purchased from Roche Applied Science (Indianapolis, IN, USA). Sal (C14H20O7, CAS#: 10338–51–9, purity > 98%) was purchased from Nanjing Zelang Medical Technological Co. Ltd (Nanjing, China). H-89 dihydrochloride (inhibitor of PKA, HY-15979A) was purchased from Med Chem Express (MCE, New Jersey, USA). Specific antibodies against synapsin I (ab8) and PSD95 (ab18258) were purchased from Abcam (Cambridge Science Park, Cambridge, UK). Specific antibodies against cAMP (DF7741), PKA (AF5450), CREB (AF6188), phosphorylated (p)-CREB (AF3189), FGF2 (DF6038), FGFR1 (AF6156), TNF-α (AF7014), IL-1β (AF5103), IL-6 (DF6087), c-caspase 3 (BF0711), Bcl-2 (AF6139), Bax (AF0120), synaptotagmin (AF6224) and NeuN (DF6145) were obtained from Affinity Biosciences (Cincinnati, OH, USA). Another NeuN antibody (MAB377) was purchased from Millipore (Billerica, MA, USA).
Af5450
Manufactured by Affinity Biosciences
Sourced in United States, China
The AF5450 is a laboratory instrument designed for cell separation and isolation. It utilizes an advanced technology to efficiently separate different cell types from complex biological samples. The core function of this equipment is to enable researchers and scientists to isolate specific cell populations for further analysis and experimentation.
Automatically generated - may contain errors
Lab products found in correlation
Dulbecco s modified eagle s medium dmem, by Thermo Fisher Scientific (1 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Mab377, by Merck Group (1 mentions)
Cell counting kit 8 cck 8, by Dojindo Laboratories (1 mentions)
In situ cell death detection kit, by Roche (1 mentions)
Hy 15979a, by MedChemExpress (1 mentions)
H 89 dihydrochloride, by MedChemExpress (1 mentions)
Af6188, by Affinity Biosciences (1 mentions)
Phosphatase inhibitor, by Roche (1 mentions)
Proteinase inhibitor, by Roche (1 mentions)
Af5395, by Affinity Biosciences (1 mentions)
Bs13278, by Bioworld Technology (1 mentions)
Af4001, by Affinity Biosciences (1 mentions)
Af6456, by Affinity Biosciences (1 mentions)
Sa00001 3, by Proteintech (1 mentions)
Nb100 2828, by Novus Biologicals (1 mentions)
Sa00001 1, by Proteintech (1 mentions)
2 protocols using af5450
1
Rat Adrenal Pheochromocytoma Cell Culture
2
Protein Expression Analysis by Western Blot
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Total proteins were extracted using radioimmunoprecipitation assay lysis buffer containing proteinase inhibitors (No. 04693159001, Roche Diagnostics, Switzerland), and phosphatase inhibitors (No. 04906845001, Roche), separated by sodium dodecyl-sulfate polyacrylamide gel electrophoresis (SDS-PAGE), transferred to polyvinylidene difluoride (PVDF) membranes, and analyzed by immunoblotting. Primary antibodies against the following proteins were used: TH (66334-1-Ig, Proteintech), protein kinase A (PKA) (AF5450, Affinity (China)), p-HSL (AF8026, Affinity), hormone-sensitive lipase (HSL) (AF6403, Affinity), p-p38 mitogen-activated protein kinase (MAPK) (AF4001, Affinity), p38 MAPK (AF6456, Affinity), PGC1α (AF5395, Affinity), UCP1 (NB100-2828, Novus Biologicals), and GAPDH (60004-1-Ig, Proteintech). The antibodies were diluted 1:1,000 with PBS. The membranes were then incubated with a peroxidase-conjugated secondary antibody (BS13278, Bioworld (China); SA00001-1, Proteintech; SA00001-3, Proteintech), and antibody-specific signals were detected by enhanced chemiluminescence and quantified using the Automatic Chemiluminescence Imaging System (Complex 2000; Nanjing PuoAoXin Biotechnology, China).
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