Wild-type (WT) C57BL/6 (Daehan Biolink, Seoul, Korea) and TCRδ−/− mice (9 (link)) (JAX stock #002119, Jackson Lab) were maintained on an 8:16-h light:dark cycle in an animal environmental control chamber (Daehan Biolink). Mice used in this study were 8–20 wk of age. Animal care was performed in accordance with the institutional guidelines set forth by Kangwon National University.
Genomic DNA was isolated using a genomic DNA purification kit according to the manufacturer's protocol (Promega, Madison, WI, USA). To confirm either WT or TCRδ knockout genotype, PCR was performed with 4 primers: TCRδ mutant forward primer, oIMR6916, 5′-CTT GGG TGG AGA GGC TAT TC-3′; reverse primer, oIMR6917, 5′-AGG TGA GAT GAC AGG AGA TC-3′; internal positive control forward primer, oIMR8744, 5′-CAA ATG TTG CTT GTC TGG TG-3′; and reverse primer, oIMR8745, 5′-GTC AGT CGA GTG CAC AGT TT-3′. The WT and TCRδ knockout alleles resulted in a 200 bp and 280 bp product, respectively.
Genomic DNA was isolated using a genomic DNA purification kit according to the manufacturer's protocol (Promega, Madison, WI, USA). To confirm either WT or TCRδ knockout genotype, PCR was performed with 4 primers: TCRδ mutant forward primer, oIMR6916, 5′-CTT GGG TGG AGA GGC TAT TC-3′; reverse primer, oIMR6917, 5′-AGG TGA GAT GAC AGG AGA TC-3′; internal positive control forward primer, oIMR8744, 5′-CAA ATG TTG CTT GTC TGG TG-3′; and reverse primer, oIMR8745, 5′-GTC AGT CGA GTG CAC AGT TT-3′. The WT and TCRδ knockout alleles resulted in a 200 bp and 280 bp product, respectively.