Complete embryos before 14.5 dpc and hearts from older time points were fixed by immersion overnight in saline phosphate buffer containing 4% paraformaldehyde and paraffin embedded. Tissue sections 3 μm thick, were deparaffinized and rehydrated. Antigen retrieval was carried out in a pressure chamber for 5 min in Diva decloaker citrate buffer (Biocare, Pike Lane Concord, CA). Non-specific binding sites were blocked with 10% goat serum for 1 h at room temperature. Tissue slices were incubated overnight at 4 °C with anti-AR polyclonal antibody diluted 1:50 (Santa Cruz Biotechnology, Santa Cruz, CA). AR antibody evaluated by Western blot technique binds to a 110 kDa protein, the expected size of androgen receptor. Slides were further incubated with Mach2 rabbit HRP polymer (Biocare) for 1 h at room temperature. Signal detection was achieved with diaminobencidin chromogen kit (Biocare). Color development was stopped by PBS rinsing and counterstained with Gill’s hematoxylin. Samples without first AR antibody were used as negative controls. Histological sections of testis were used as positive controls.
Anti ar polyclonal antibody
Manufactured by Santa Cruz Biotechnology
The Anti-AR polyclonal antibody is a laboratory reagent used to detect and quantify the presence of androgen receptor (AR) proteins in biological samples. It is a polyclonal antibody produced by immunizing animals with a specific antigen derived from the AR protein. This antibody can be used in various techniques, such as western blotting, immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA), to identify and analyze the expression levels of AR in different cell types or tissues.
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Lab products found in correlation
Mach 2 rabbit hrp polymer, by Biocare Medical (1 mentions)
Bis tris gel, by Thermo Fisher Scientific (1 mentions)
Anti β actin monoclonal antibody, by Cell Signaling Technology (1 mentions)
Protease and phosphatase inhibitor cocktail, by Merck Group (1 mentions)
Amersham ecl western blot detection reagent, by GE Healthcare (1 mentions)
Anti psa polyclonal antibody, by Agilent Technologies (1 mentions)
2 protocols using anti ar polyclonal antibody
1
Immunohistochemical Analysis of Androgen Receptor
2
Western Blot Analysis of Prostate Cancer Markers
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Cells from the cell culture experiments were lysed in Mammalian Protein Extraction Reagent (M-PER) (Pierce from Thermo Fisher Scientific Inc.) in the presence of protease and phosphatase inhibitor cocktails (Sigma-Aldrich). Cell lysates equivalent to 25 μg of total protein were separated on 4–12% Bis-Tris gel (Life Technologies) and transferred to PVDF membrane. Membranes were incubated with primary antibodies: anti-PMEPA1 monoclonal antibody (Novus Biologicals), anti-GSTP1 polyclonal antibody (US Biologicals), anti-β-Actin monoclonal antibody (Cell Signaling), anti-PSA polyclonal antibody (Dako) and anti-AR polyclonal antibody (Santa Cruz Biotech) at 4 °C overnight and were washed before being treated with respective secondary antibodies (GE Healthcare Biosciences). Western blots were visualized by the Amersham ECL western blot detection reagent (GE Healthcare Biosciences).45 (link)
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