Dna marker 2000

The instrumentation used for the study is described in the Supplementary Materials S1. The bacterial DNA extraction kit, goat anti-mouse IgG antibody, rabbit anti-6-FAM polyclonal antibody (6-FAM), and anti-digoxin antibody used in this paper were purchased from Tiangen Biotech Co., Ltd. (Shanghai, China), BBI Sciences Corporation (Shanghai, China), Otwo Biotech (Shenzhen, China), and Abbkine (Wuhan, China), respectively. DNA marker 2000, isothermal amplification PCR mix, and bovine serum albumin (BSA) were provided by Sangong Biotech Co., Ltd. (Shanghai, China). SYTO9, streptavidin (SA) and polyethylenimine (PEI), MES monohydrate (MES), N(3-dimethylaminopropyl)-N’-ethylcarbodiimide hydrochloride (EDC), N-hydroxysuccinimide (NHS), tetraethoxysilane (TEOS) and Tween-20 used in the synthesis of materials were obtained from Sigma-Aldrich (St. Louis, MO, USA). CdSe/ZnS-MPA QDs were ordered from Mesolight Inc. (Suzhou, China). The nitrocellulose membrane utilised in the immunochromatographic strips was procured from Sartorius (Madrid, Spain), while the remaining materials were sourced from Jieyi Biotechnology Co. (Shenzhen, China).

BamHI restriction endonuclease, T4 DNA ligase, 2×TransStart Fast Fly SuperMix, DMT enzyme and DMT-competent cells were all provided by TransGen Biotech Co., Ltd; Ampicillin (Amp), DNA marker 2000, SanPrep column plasmid DNA Mini-Preps extraction kit and primer synthesis were all provided by Sangon Biotech (Shanghai) Co.,Ltd.; the target gene BCKD and plasmid vector pGEX-4T1 were provided by University of California, Davis; ddH2O and other materials are prepared by the lab of environmental science and engineering, Yangzhou University.