Bl21 codonplus de3 cells

Manufactured by Thermo Fisher Scientific

BL21-CodonPlus [DE3] cells are Escherichia coli (E. coli) strains that are engineered for enhanced protein expression. They are designed to improve the translation of heterologous genes by providing additional copies of rare tRNA genes.

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Lab products found in correlation

Hitrap, by Cytiva (3 mentions) Akta 900 fast protein liquid chromatography, by GE Healthcare (2 mentions) Complete his tag purification resin, by Roche (1 mentions) Hi trap affinity columns, by GE Healthcare (1 mentions) Gst trap hp columns, by GE Healthcare (1 mentions)

Spelling variants of this product

BL21(DE3) (201 citations) BL21(DE3) cells (59 citations) BL21(DE3)CodonPlus-RIL cells (4 citations)

3 protocols using bl21 codonplus de3 cells

Purification and Antibody Generation for Drosophila Rab11

Cited 2 times

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GST-full-length Drosophila Rab11 was expressed in BL21-CodonPlus [DE3] cells (Invitrogen) and purified using HiTrap affinity columns (GSTtrap FF, and GSTtrap HP columns, GE Healthcare) operated with AKTA 900 Fast Protein Liquid Chromatography as previously described [13 (link)]. Polyclonal antisera were raised against the purified GST-Rab11 protein. Polyclonal anti-anillin antibodies were raised against the N-terminal 270 amino acids of anillin, following the procedure described in [69 (link)]. Immunization was carried out at Agro-Bio Services (www.agro-bio.com) using standard procedures. The anti-GST-Rab11 and anti-anillin antisera were depleted of anti-GST antibodies and affinity-purified against either GST-Rab11 or GST-anillin before use in immunoblotting.

Giansanti M.G., Vanderleest T.E., Jewett C.E., Sechi S., Frappaolo A., Fabian L., Robinett C.C., Brill J.A., Loerke D., Fuller M.T, & Blankenship J.T. (2015). Exocyst-Dependent Membrane Addition Is Required for Anaphase Cell Elongation and Cytokinesis in Drosophila. PLoS Genetics, 11(11), e1005632.

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Purification and Antibody Generation for Drosophila Rab1 and GOLPH3

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GST-full-length Drosophila Rab1 protein was expressed in BL21-CodonPlus (DE3) cells (Invitrogen) and purified using HiTrap affinity columns (GTtrap FF, and GSTtrap HP columns, GE Healthcare) operated with AKTA 900 fast protein liquid chromatography. Polyclonal antisera against the purified GST-Rab1 protein were produced in rabbits and mice (Agro-Bio Services; www.agro-bio.com). The anti-GST-Rab1 antisera were first depleted of anti-GST antibodies and then affinity-purified against GST-Rab1. The pET Directional TOPO Expression kit (Thermofisher Scientific) was used to obtain 6XHis-GOLPH3. 6XHis-GOLPH3 was purified using cOmplete His-Tag Purification Resin (Roche). The following antibodies were used in the assays of this study: mouse anti-Rab1 antibody S12085a and rabbit anti-Rab1 L12085a/169 antibody.

Sechi S., Frappaolo A., Fraschini R., Capalbo L., Gottardo M., Belloni G., Glover D.M., Wainman A, & Giansanti M.G. (2017). Rab1 interacts with GOLPH3 and controls Golgi structure and contractile ring constriction during cytokinesis in Drosophila melanogaster. Open Biology, 7(1), 160257.

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Purification and Antibody Production of Drosophila GOLPH3

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GST-full-length Drosophila GOLPH3 (dGOLPH3) proteins and GST-full-length Drosophila Rab11 were expressed in BL21-CodonPlus [DE3] cells (Invitrogen) and purified using HiTrap affinity columns (GTtrap FF, and GSTtrap HP columns, GE Healthcare) operated with AKTA 900 Fast Protein Liquid Chromatography. Polyclonal antisera were raised against the purified GST-dGOLPH3 protein. Immunization was carried out at Agro-Bio Services (www.agro-bio.com); two rabbits and two mice were injected using standard procedures. The anti-GST-GOLPH3 antisera were first depleted of anti-GST antibodies and then affinity-purified against GST-GOLPH3.

Sechi S., Colotti G., Belloni G., Mattei V., Frappaolo A., Raffa G.D., Fuller M.T, & Giansanti M.G. (2014). GOLPH3 Is Essential for Contractile Ring Formation and Rab11 Localization to the Cleavage Site during Cytokinesis in Drosophila melanogaster. PLoS Genetics, 10(5), e1004305.

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