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Pmirglo basic luciferase reporter vector

Manufactured by Promega
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Sourced in United States

PmirGLO-basic luciferase reporter vector is a plasmid that contains a firefly luciferase gene as a reporter. The vector provides a basic platform for constructing reporter gene assays to study gene expression and regulation.

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Lab products found in correlation

Dual luciferase reporter assay system, by Promega (2 mentions) Renilla luciferase vector, by Promega (1 mentions) Lipofectamine 2000, by Thermo Fisher Scientific (1 mentions) Transfection reagent, by Roche (1 mentions) Luciferase assay system, by Promega (1 mentions) Dual luciferase reagent, by Promega (1 mentions) Mirna mimic, by Bioneer (1 mentions) Lipofectamine 3000, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

PmirGLO‐basic reporter vector PmirGLO luciferase reporter vector PmirGLO luciferase reporter PmirGLO basic vector PmirGLO reporter vector PmirGLO luciferase vector Luciferase reporter vector PmirGLO reporter PmirGLO vector Reporter vectors

4 protocols using pmirglo basic luciferase reporter vector

1

Luciferase Assay for miR-486 Binding

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The SNHG15 fragments, containing the wildtype (SNHG15‐WT) or mutant type (SNHG15‐Mut) binding sites with miR‐486 were synthesized by PCR and cloned into the pmirGLO‐basic luciferase reporter vector (Promega, Madison, WI). Briefly, NSCLC cells were co‐transfected with combined luciferase reporter vectors (100 ng), Renilla luciferase vector (20 ng) (Promega), and miR‐486 mimics (100 nM) or miR‐NC using Lipofectamine 2000 (Invitrogen). At 48 hr of transfection, the luciferase activity was tested using the Dual‐Luciferase Reporter assay system (Promega) normalized to Renilla luciferase activity.
Jin B., Jin H., Wu H., Xu J, & Li B. (2018). Long non‐coding RNA SNHG15 promotes CDK14 expression via miR‐486 to accelerate non‐small cell lung cancer cells progression and metastasis. Journal of Cellular Physiology, 233(9), 7164-7172.
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2

Luciferase Assay of JARID1B UTR

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The human JARID1B 3′-untranslated region (UTR) and corresponding mutants were cloned into the pMIR-GLO basic luciferase reporter vector (Promega, Madison, WI, USA). GC cells were transfected with the internal control vector pRL-TK and the promoter or 3′-UTR reporters using Roche Transfection Reagent (Roche, Basel, Switzerland) 24 h after seeding. Luciferase reporter activity was measured by a Luciferase Assay System (Promega) as per the manufacturer’s instructions 48 h after transfection.
Zheng L., Wu Y., Shen L., Liang X., Yang Z., Li S., Li T., Shang W., Shao W., Wang Y., Liu F., Ma L, & Jia J. (2021). Mechanisms of JARID1B Up-Regulation and Its Role in Helicobacter pylori-Induced Gastric Carcinogenesis. Frontiers in Oncology, 11, 757497.
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3

Luciferase Assay for LINC02418 and MELK

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The complementary DNA fragments from the wild-type or mutant LINC02418 and MELK fragments were subcloned and inserted downstream of the luciferase gene within the pmirGLO-Basic luciferase reporter vector (Promega). Luciferase reporter vectors were stably co-transfected with scrambled negative control siRNAs as follows: miR-1273g-3p, miR-542-3p, miR-5186, miR-2277-3p, miR-3192-3p, miR-3193, and miR-4693-3p were co-transfected into SW1116 and HT29 cells. Luciferase activities were measured using a dual-luciferase reagent (Promega).
Zhao Y., Du T., Du L., Li P., Li J., Duan W., Wang Y, & Wang C. (2019). Long noncoding RNA LINC02418 regulates MELK expression by acting as a ceRNA and may serve as a diagnostic marker for colorectal cancer. Cell Death & Disease, 10(8), 568.
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4

Luciferase Reporter Assay for miRNA Binding

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293T cells were grown in high glucose DMEM supplemented with 10% FBS, penicillin at 37°C, and 5% CO2. 293T cells were transiently transfected using Lipofectamine 3000 (Invitrogen Life Technologies). Transfection of each 3’ UTR into a pmirGLO basic luciferase reporter vector (Promega; 300 ng) and each mimic miRNA (Bioneer, Daejeon, Korea; 25 pg) was performed in a 24‐well plate. Luciferase assay was performed at 48 hours post‐transfection. Renilla luciferase and firefly luciferase activities were analysed using the Dual‐Luciferase® Reporter Assay System (Promega) following the manufacturer's instructions. The firefly luciferase activities were normalized using Renilla luciferase activity.
Kim Y.S., Yang S.C., Park M., Choi Y., DeMayo F.J., Lydon J.P., Kim H., Lim H.J, & Song H. (2021). Different Cre systems induce differential microRNA landscapes and abnormalities in the female reproductive tracts of Dgcr8 conditional knockout mice. Cell Proliferation, 54(3), e12996.
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