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Anti fas cd95

Manufactured by Thermo Fisher Scientific

Anti-Fas (CD95) is a laboratory reagent used for the detection and analysis of Fas (CD95) receptor, a cell surface protein involved in the regulation of apoptosis (programmed cell death). This product is intended for research use only and its core function is to provide a specific and sensitive detection of the Fas (CD95) receptor in various cell and tissue samples.

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2 protocols using anti fas cd95

1

Multiparametric Flow Cytometry of Lymphocytes

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Cells suspended in PBS supplemented with 0.5% w/v bovine albumin serum (BSA) and 2 mM EDTA were stained with combinations of the following antibodies: anti-CD19 (BD Biosciences, BV421), anti-GL7 (eBioscience, Alexa488), anti-Fas (CD95) (eBioscience, APC), anti-B220 (CD45R) (BioLegend, BV711 & APC, BD Biosciences, BUV496), anti-CD38 (BD Biosciences, BUV395), anti-CXCR4 (eBioscience, PE/Cy7), anti-CD86 (BD Biosciences, BV421, eBiosciences, FITC), anti-IgG1 (eBioscience, PE/Cy7), anti-CD69 (BioLegend, APC), anti-IgM (BD Biosciences, BV711), and anti-MHCII (eBioscience, PE/Cy5). Typically, 0.06–1 μg of antibody was used for staining of approximately 105–107 cells in 100 μL. All samples were analyzed using a BD Accuri C6 or BD LSRFortessa (BD Biosciences, Franklin Lakes, NJ, USA). The data were analyzed using FlowJo software.
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2

Multiparametric Flow Cytometry of Lymphocytes

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells suspended in PBS supplemented with 0.5% w/v bovine albumin serum (BSA) and 2 mM EDTA were stained with combinations of the following antibodies: anti-CD19 (BD Biosciences, BV421), anti-GL7 (eBioscience, Alexa488), anti-Fas (CD95) (eBioscience, APC), anti-B220 (CD45R) (BioLegend, BV711 & APC, BD Biosciences, BUV496), anti-CD38 (BD Biosciences, BUV395), anti-CXCR4 (eBioscience, PE/Cy7), anti-CD86 (BD Biosciences, BV421, eBiosciences, FITC), anti-IgG1 (eBioscience, PE/Cy7), anti-CD69 (BioLegend, APC), anti-IgM (BD Biosciences, BV711), and anti-MHCII (eBioscience, PE/Cy5). Typically, 0.06–1 μg of antibody was used for staining of approximately 105–107 cells in 100 μL. All samples were analyzed using a BD Accuri C6 or BD LSRFortessa (BD Biosciences, Franklin Lakes, NJ, USA). The data were analyzed using FlowJo software.
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