Each Eppendorf tube was weighed by the electronic microbalance (Sartorius, Germany Model-CP225D) of 10-5g sensitivity. Three consecutive weighings were conducted for each tube and their arithmetic mean was regarded as the weight of each empty Eppendorf tube.
Model cp225d
Manufactured by Sartorius
Sourced in Germany
The Model-CP225D is a precision balance from Sartorius, designed for laboratory use. It features a weighing capacity of up to 220 grams and a readability of 0.01 milligrams. The balance is equipped with a backlit LCD display and offers various weighing units, including milligram, gram, and carat.
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Lab products found in correlation
5 protocols using model cp225d
1
Precise Weighing of Eppendorf Tubes
2
Measuring Apical Debris Quantification
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The experimental model used for this in vitro study was Myers and Montgomery model [24 (link)]. Eppendorf tubes were used to collect the apical debris. Empty Eppendorf tubes were preweighed by the electronic microbalance (Sartorius, Germany Model-CP225D) of 10−5 gm sensitivity. The 60 Eppendorf tubes (20 Eppendorf tubes for the 20 teeth per group) were preweighed three consecutive times and the arithmetic mean values were recorded. The teeth were held to a pre-weighed Eppendorf tube using a rubber stopper, and this assembly was fixed to a glass container wrapped using aluminum foil to avoid observer bias by the operator during the instrumentation (Figure 1 ). Then a 30-gauge (BD microlance, India) needle was used to vent and equalize internal and external pressure within the glass container.
3
Quantification of Δ9-THC in Nanoparticles
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The determination of the drug content of the NPs was carried out as previously described12 (link) . Around 5 mg of lyophilized NPs were accurately weighed using a high-precision analytical balance (d = 0.01 mg; Model CP 225D; Sartorius AG, Göttingen, Germany). Following, 5 mL of acetone were added and the mixture was accurately vortexed to dissolve the particles in the organic phase. Acetone was then evaporated using a rotavapor (Büchi R/210, BüchiLabortechnik AG, Flawil, Switzerland). Next, 1 mL of ethanol was added and the Δ9-THC content solved under sonication (Branson 50 W, Branson Ultrasonics Corporation, Danbury, CT, USA) and vortexing for 5 min. The obtained drug solution, was then filtered (0.22 µm syringe filter, Millex® GV, Millipore, Barcelona, Spain) and injected into the HPLC system for Δ9-THC detection following a validated method10 .
The drug content was expressed as entrapment efficiency (EE %) and drug loading (DL %) following the Eqs. (1 and 2 ):
The drug content was expressed as entrapment efficiency (EE %) and drug loading (DL %) following the Eqs. (
4
Water Permeability of Composites
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The water permeability of the composites was evaluated according to the EN 15801 Standard on three cylindrical specimens with 35 mm diameter and 100 mm length, cured for 28 days at 23 ± 1 °C. A Sartorius balance Model CP225D (Sartorius, Gottingen, Germany) was used to evaluate the moisture absorption as a function of time.
5
Quantification and Stability of U-AZT Nanoparticles
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To determine the content of U-AZT in the powders, the nanoparticles (~ 2 mg) were weighted using a high precision analytical balance (d= 0.01 mg; Sartorius, model CP 225D, Goettingen, Germany) and dissolved in MeOH (0.38 mg/mL); thereafter the samples were diluted 1:2 with water and filtered aliquots of these solutions (0.45 µm) were injected into a HPLC system for U-AZT assay. The percent drug loading in nanoparticle was calculated according to the following equation:
All the reported values represent the mean of three independent experiments.
[1]
In order to evaluate the stability of the nanoparticles in serum, 10 mg of liophilized nano-tauro and nano-UDCA were added to 1 mL of fetal bovine serum (FBS) solution (100% FBS; 37°C) [25] (link).
At selected time points, ranging from 15 min to 5 h, aliquots of the suspensions were diluted with MilliQ water (1/10 v/v) and size, PDI and zeta potential were measured by the DLS analysis (Malvern Z-Sizer, Zetasizer version 6.12, Malvern Instruments, Worcs, U.K). Each measurement was performed in triplicate.
All the reported values represent the mean of three independent experiments.
[1]
In order to evaluate the stability of the nanoparticles in serum, 10 mg of liophilized nano-tauro and nano-UDCA were added to 1 mL of fetal bovine serum (FBS) solution (100% FBS; 37°C) [25] (link).
At selected time points, ranging from 15 min to 5 h, aliquots of the suspensions were diluted with MilliQ water (1/10 v/v) and size, PDI and zeta potential were measured by the DLS analysis (Malvern Z-Sizer, Zetasizer version 6.12, Malvern Instruments, Worcs, U.K). Each measurement was performed in triplicate.
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