hASC‐HLCs or HUVECs were plated in antibiotic‐free basal medium 24 hrs prior to transfection. siRNA transfection was performed following the manufacturer's protocol as previously described. Briefly, ON‐TARGET SMARTpool siRNAs directed against human α5 integrin (L‐008003‐00‐0005, Dharmacon, Lafayette, LA, USA), human FN 1 (L‐009853‐00‐0005, Dharmacon), human Src (L‐003175‐00‐0005, Dharmacon) or non‐targeting siRNAs (D‐001810‐10‐05, Dharmacon) were mixed with Transfection DharmaFECT 4 (Dharmacon) or Transfection DharmaFECT 1 (Dharmacon). After a 20‐min. incubation at room temperature, the complexes were added to the cells at a final siRNA concentration of 50 nM. The medium was replenished with medium containing antibiotics for 24 hrs after transfection. The culture medium was changed every 2 days for the duration of the experiment.
Transfection dharmafect 4
Manufactured by Horizon Discovery
Sourced in United States
DharmaFECT 4 is a high-performance transfection reagent for the delivery of siRNA and other nucleic acids into a variety of cell types. It is designed to provide efficient transfection with low cytotoxicity.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using transfection dharmafect 4
1
siRNA-Mediated Knockdown of Integrin, Fibronectin, and Src
2
Integrin and SRC Silencing Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
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HLCs and L02 cells were plated in antibiotic-free basal medium 24 h prior to transfection. The siRNA transfection was performed following the manufacturer’s protocol as previously described. Briefly, ON-TARGET SMARTpool siRNAs directed against human α5 integrin (L-008003-00-0005, Dharmacon, Lafayette, LA, USA), human SRC (L-003175-00-0005, Dharmacon) or non-targeting siRNAs (D-001810-10-05, Dharmacon) were mixed with Transfection DharmaFECT 4 (Dharmacon). After a 20-min incubation at room temperature, the complexes were added to the cells at a final siRNA concentration of 50 nM. The medium was replenished with fresh medium for 24 h post-transfection. Experiments were performed 48–72 h after transfection.
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