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2 protocols using cyclopropyl fentanyl

1

Analytical Standards for Opioid Detection

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Carfentanil, cocaine, fentanyl, heroin, oxycodone, and remifentanil were purchased as analytical standards (100 μg/mL diluted in methanol) from Cerilliant Corporation (Round Rock, TX). Acetyl fentanyl, butyryl fentanyl, crotonyl fentanyl, cyclopropyl fentanyl, p-fluoroisobutyryl fentanyl (FIBF), 2-furanyl fentanyl, β-hydroxythiol fentanyl, methoxy fentanyl, methyl fentanyl, norfentanyl, and U-47700 were purchased as analytical standards (100 μg/mL diluted in methanol) from Cayman Chemical (Ann Arbor, MI). A complete list of test compounds used in this study is shown in Table 1.
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2

Cyclopropylfentanyl Identification Protocol

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Cyclopropylfentanyl was obtained from Cayman Chemicals (Ann Arbor, MI, USA). Liquid chromatography–mass spectrometry (LC-MS) grade acetonitrile, water and formic acid used for the LC quadrupole time-of-flight MS (LC-QTOF-MS) were obtained from Fisher Scientific (Gothenburg, Sweden), while the ammonium formate (Fluka) was obtained from Sigma-Aldrich (Stockholm, Sweden). β-glucuronidase/arylsulfatase (Helix promatia, 4.5 U/mL and 14 U/mL, respectively) for urine treatment was purchased from Roche (Mannheim, Germany).
Gradient grade acetonitrile, methanol, formic acid p.a. (98%) and sodium acetate p.a. were purchased from Merck (Darmstadt, Germany). Water was produced in-house with a MilliQ Gradient 10 production unit (Millipore, Billerica, MA). Chemicals used for the synthesis of reference materials are described in the supplemental material.
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