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Rabbit gmf polyclonal antibody

Manufactured by Proteintech
Sourced in United States

The Rabbit GMF polyclonal antibody is a research-grade antibody produced in rabbits. It is designed to detect the target protein in various applications, such as Western blotting, immunohistochemistry, and ELISA. This antibody is purified and supplied in a liquid format.

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2 protocols using rabbit gmf polyclonal antibody

1

Antibody Characterization of Neuroinflammation

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Rabbit GMF polyclonal antibody and mouse GMF monoclonal antibodies were purchased from Protein Tech (Chicago, IL, USA). Mouse anti-6E10 (Bio Legend San Diego, CA, USA), mouse anti-phospho-tau (EMD Millipore, Massachusetts, USA), mouse anti-NLRP3 (AdipoGen, San Diego, CA, USA), rabbit polyclonal anti-caspase-1(Santa Cruz, Dallas TX, USA), rabbit polyclonal anti-IL-1β (Abcam, Cambridge, MA, USA), mouse anti-IL-18 (Santa Cruz, Dallas TX, USA), rabbit anti-SQSTM1/p62 (Abcam, Cambridge, MA, USA), rabbit ant-LC3(Abcam, Cambridge, MA, USA) and rabbit anti-LAMP1(Abcam, Cambridge, MA, USA) and mounting medium with 4′,6-diamidino-2-phenylindole (DAPI) (H-1200; Vector Laboratories, Inc.Burlingame, CA, USA).
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2

Immunohistochemical Analysis of Mitochondrial Proteins

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Rabbit GMF polyclonal antibody and mouse GMF monoclonal antibodies were purchased from Protein Tech (Chicago, IL, USA). The vendors for other antibodies are listed within parentheses. Anti-UCP2 polyclonal antibody (Calbiochem Millipore, San Diego, CA, USA), Rabbit polyclonal UCP4 antibody (Novus Biologicals, Littleton, CO, USA), anti-rabbit iNOS and rabbit polyclonal to NF-κB p65 (Abcam, Cambridge, MA, USA) were obtained. Vectastain avidin biotin peroxidase complex (ABC) reagents and kits (peroxidase mouse IgG/ peroxidase rabbit IgG) and Impact 3,3’-diaminobenzidine (DAB) peroxidase substrate kits were from Vector Labs (Burlingame, CA, USA). Additional reagents include phosphate buffered saline (PBS; GIBCO, Life Technologies, Grand Island, NY, USA), Thioflavin-S (Sigma, St Louis, MO, USA). Permount (Fisher Scientific (Pittsburgh, PA, USA) and Fluorogel (EM Sciences, Hatfield, PA, USA).
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