Log In Sign up
The largest database of trusted experimental protocols

Csu x1 m1 spinning disk

Manufactured by Sutter Instruments
Visit Supplier

The CSU-X1 M1 is a spinning disk confocal microscope system. It is designed to provide high-speed, high-sensitivity imaging of live cells and tissues. The system uses a rapidly spinning disk to generate multiple excitation points, enabling parallel acquisition of images. This results in reduced photobleaching and phototoxicity compared to traditional laser scanning confocal microscopes.

Automatically generated - may contain errors

Lab products found in correlation

Plan apochromat oil objective, by Zeiss (2 mentions) Zen 2, by Zeiss (2 mentions) Widefield system, by Nikon (2 mentions) Lsm 880 confocal microscope, by Zeiss (2 mentions) Alpha plan apochromat oil objective, by Zeiss (2 mentions)

2 protocols using csu x1 m1 spinning disk

1

Super-resolution Imaging of Cellular Organelles

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were maintained at 37°C and 5% CO2 (see Supplementary Data Table 2). Live cell Airyscan superresolution microscopy used a Zeiss LSM 880 confocal microscope with Airyscan detector and 63× Plan-Apochromat oil objective, NA 1.4. Sequential excitation of fluorophores was used, with 488nm laser line for Snap-Cell Oregon Green and MitoSOX, and 633nm laser for Snap-Cell 647-SiR, with appropriate dual bandpass filters. The Airyscan detector was adjusted regularly, and images were Airyscan processed in 2D using the Zeiss Zen 2.3 software package (Carl Zeiss Microscopy).
For spinning disk confocal imaging, cells were imaged on a 3i Marianas platform with a Zeiss Axio Observer Z1, Optovar 1×, 1.6× and 2.5×, with a Yokogawa CSU-X1 M1 spinning disk, Sutter LB-10W fast 10-position filter wheel with filters: 445/45, 525/30, 617/73, and Andor Neo sCMOS camera using a 63× Alpha Plan-Apochromat oil objective, NA 1.46 (Carl Zeiss Microscopy), using Slidebook 5.5 acquisition software (3i) and lasers: Violet (solid state 405nm/100mW), Blue (solid state 488nm/150mW), and Lime (solid state 561nm/50mW).
An inverted Nikon widefield system with Andor Zyla 4.2+ camera, external filter wheel and SpectraX LED was used for widefield imaging. Images were acquired using a 60× water objective, NA 1.20, and NIS-Elements 4.5 software.
Döhla J., Kuuluvainen E., Gebert N., Amaral A., Englund J.I., Gopalakrishnan S., Konovalova S., Nieminen A.I., Salminen E.S., Muñumer R.T., Ahlqvist K., Yang Y., Bui H., Otonkoski T., Käkelä R., Hietakangas V., Tyynismaa H., Ori A, & Katajisto P. (2022). Metabolic determination of cell fate through selective inheritance of mitochondria. Nature cell biology, 24(2), 148-154.
+ Open protocol
+ Expand
2

Super-resolution Imaging of Cellular Organelles

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were maintained at 37°C and 5% CO2 (see Supplementary Data Table 2). Live cell Airyscan superresolution microscopy used a Zeiss LSM 880 confocal microscope with Airyscan detector and 63× Plan-Apochromat oil objective, NA 1.4. Sequential excitation of fluorophores was used, with 488nm laser line for Snap-Cell Oregon Green and MitoSOX, and 633nm laser for Snap-Cell 647-SiR, with appropriate dual bandpass filters. The Airyscan detector was adjusted regularly, and images were Airyscan processed in 2D using the Zeiss Zen 2.3 software package (Carl Zeiss Microscopy).
For spinning disk confocal imaging, cells were imaged on a 3i Marianas platform with a Zeiss Axio Observer Z1, Optovar 1×, 1.6× and 2.5×, with a Yokogawa CSU-X1 M1 spinning disk, Sutter LB-10W fast 10-position filter wheel with filters: 445/45, 525/30, 617/73, and Andor Neo sCMOS camera using a 63× Alpha Plan-Apochromat oil objective, NA 1.46 (Carl Zeiss Microscopy), using Slidebook 5.5 acquisition software (3i) and lasers: Violet (solid state 405nm/100mW), Blue (solid state 488nm/150mW), and Lime (solid state 561nm/50mW).
An inverted Nikon widefield system with Andor Zyla 4.2+ camera, external filter wheel and SpectraX LED was used for widefield imaging. Images were acquired using a 60× water objective, NA 1.20, and NIS-Elements 4.5 software.
Döhla J., Kuuluvainen E., Gebert N., Amaral A., Englund J.I., Gopalakrishnan S., Konovalova S., Nieminen A.I., Salminen E.S., Muñumer R.T., Ahlqvist K., Yang Y., Bui H., Otonkoski T., Käkelä R., Hietakangas V., Tyynismaa H., Ori A, & Katajisto P. (2022). Metabolic determination of cell fate through selective inheritance of mitochondria. Nature cell biology, 24(2), 148-154.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!