Chitosan, 3,4-dihydroxy hydrocinnamic acid, 1-ethyl-3-(3-dimethylamino-propyl)-carbodiimide hydrochloride (EDC), and dextran were purchased from Aladdin Reagents. Sodium periodate (NaIO4) was purchased from Acros. Trametinib was purchased from Selleck. The TRAP staining kit was purchased from Wako. The RNA extraction kit was purchased from Chengdu Forge Biotechnology Co., Ltd. (China). HiFiScript gDNA Removal RT MasterMix and MagicSYBR Mixture were purchased from Beijing CWBIO Biotechnology Co., Ltd. (China). The BCA protein quantification kit and ultrasensitive ECL developer were purchased from Suzhou New Semi Biotechnology Co., Ltd. (China). Anti-phospho-p44/42 MAPK (ERK1/2) antibody (No. 4370) and anti-p44/42 MAPK (ERK1/2) antibody (No. 4695) were purchased from Cell Signaling Technology. Anti-GAPDH (AF1186) were purchased from Shanghai Beyotime Biotechnology Co., Ltd. (China). Anti-mouse IgG HRP-conjugated antibody and anti-rabbit IgG HRP-conjugated antibody were purchased from Cell Signaling Technology. Biotin-labeled goat anti-mouse/rabbit IgG polymer and DAB color development kits were purchased from Beijing Zhongshan Jinqiao Biotechnology Co., Ltd. (China).
Anti gapdh af1186
Manufactured by Beyotime
Sourced in China
Anti-GAPDH (AF1186) is a laboratory reagent designed for the detection of the glyceraldehyde-3-phosphate dehydrogenase (GAPDH) protein in biological samples. GAPDH is a widely used internal control and housekeeping gene in various molecular biology and cell biology applications. This antibody can be utilized in techniques such as Western blotting, immunohistochemistry, and immunocytochemistry to identify and quantify GAPDH expression levels.
Automatically generated - may contain errors
Lab products found in correlation
Hrp conjugated anti rabbit igg antibody, by Cell Signaling Technology (1 mentions)
Trap staining kit, by Fujifilm (1 mentions)
Trametinib, by Selleck Chemicals (1 mentions)
Sodium periodate naio4, by Thermo Fisher Scientific (1 mentions)
Hrp conjugated anti mouse igg antibody, by Cell Signaling Technology (1 mentions)
Ab94567, by Abcam (1 mentions)
Anti n cadherin, by Cell Signaling Technology (1 mentions)
Anti e cadherin, by Cell Signaling Technology (1 mentions)
Anti vimentin ab92547, by Abcam (1 mentions)
Polyvinylidene fluoride (pvdf), by Biosharp (1 mentions)
Bca assay kit, by Beyotime (1 mentions)
Chemidoc, by Bio-Rad (1 mentions)
Proteinase inhibitor, by Beyotime (1 mentions)
Bca kit, by Sangon (1 mentions)
3 protocols using anti gapdh af1186
1
Chitosan-Based Molecular Crosslinking
2
Protein Expression Analysis in FaDu Cells
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Total proteins were sampled from FaDu cells and tissues via a kit (KeyGen, Nanjing, China). A BCA Assay Kit (Beyotime, Shanghai, China) was utilized to examine the protein concentration. Hybrid proteins were isolated via SDS-PAGE (10%), placed onto the PVDF (polyvinylidene fluoride) membranes (Biosharp, Hefei, China), subjected to a 15-minute blockage using buffer (Beyotime, Shanghai, China), and incubated overnight using 1 : 1000 anti-RBM24 (ab94567), 1 : 5000 anti-Vimentin (ab92547; both Abcam, Cambridge, UK), 1 : 1000 anti-E-cadherin (#3195), 1 : 1000 anti-N-cadherin (#13116; both Cell Signaling Technology, MA, USA), 1 : 1000 anti-Twist1 (AF4009; Affinity, Jiangsu, China), and 1 : 3000 anti-GAPDH (AF1186; Beyotime, Shanghai, China) antibodies at 4°C. Subsequently, 1 h processing of the membranes was accomplished using 1 : 5000 anti-rabbit goat IgG (A0208; Beyotime, Shanghai, China) at ambient temperature. Finally, an XRS+ imaging system (ChemiDoc™; Bio-Rad, CA, USA) was utilized to examine the specific proteins.
3
Protein Expression Analysis Protocol
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All the cells were lysed in radioimmunoprecipitation (RIPA) buffer supplemented with 1% proteinase inhibitors (Beyotime, Shanghai, China) on ice for 30 min. Protein were extracted by centrifuged for 15 min at 4 °C and quantitatively analyzed using a BCA kit (Sangon, Shanghai, China). An equal amount of protein from each sample was separated by 10% SDS PAGE and then transferred to the PVDF membrane (BBI, USA). The membranes were incubated overnight at 4 °C with indicated primary antibodies and following incubated with the corresponding secondary antibodies. Anti-cyclinA (sc-271682), anti-cyclin D (sc-8396), anti-cyclin E (sc-377100), anti-CDK2 (sc-6248), and anti-CDK4 (sc-23896) antibodies were purchased from Santa Cruz; anti-GAPDH (AF1186) were purchased from Beyotime Biotechnology. The anti-mouse IgG secondary antibodies were purchased from Sino biological (SSA007), and anti-rabbit IgG secondary antibodies were purchased from Bioworld Technology (BS13278).
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