Expression constructs encoding for human flotillin-1 or flotillin-2 were a gift from V. Niggli (University of Bern). TCRζ-PSCFP2 was provided by Prof. K. Gaus (University of New South Wales). PA-mCherry expression backbone was obtained from Clontech. TCRζ PA-mCherry was made as described in [15 (link)]. mCherry-Rab5 was a gift from Gia Voeltz (Addgene plasmid # 49,201). mCherry-Rab11a was a gift from Michael Davidson (Addgene plasmid # 55,124). pBGPa-CMV-GFP-OSBP PH domain was a gift from Tim Levine (Addgene plasmid # 58,724). LACT-C2 and 2xFYVE expression constructs were a gift from Prof. Rob Parton (University of Queensland).
Mcherry rab11a
Manufactured by Addgene
MCherry-Rab11a is a fluorescently tagged protein that can be used to study the localization and dynamics of the Rab11a protein in live cells. Rab11a is a small GTPase that plays a role in regulating endocytic recycling pathways.
Automatically generated - may contain errors
2 protocols using mcherry rab11a
1
Fluorescent Protein Constructs for Cellular Imaging
2
Fluorescent Protein Constructs for Imaging
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TCRζ-PSCFP2 and Lck10-PA-mCherry were provided by K. Gaus (University of New South Wales). PA-mCherry expression backbone was obtained from Clontech. TCRζ-PA-mCherry and CD4-PA-mCherry were generated by inserting TCRζ or CD4 into the PA-mCherry backbone with EcoRI and AgeI. Clathrin-EGFP was kindly provided by T. Kirchausen (Harvard Medical School). mCherry-Rab5 was a gift from Gia Voeltz (Addgene plasmid #49201). mCherry-Rab7a (Addgene plasmid #55127) and mCherry-Rab11a (Addgene plasmid #55124) were gifts from Michael Davidson.
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