Polyclonal erk m5670

Manufactured by Merck Group

Polyclonal ERK (M5670) is a laboratory reagent produced by Merck Group. It is an antibody that recognizes extracellular signal-regulated kinase (ERK), a family of proteins involved in various cellular processes. The antibody can be used to detect and study the expression and activation of ERK in biological samples.

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Lab products found in correlation

Vinculin v9264, by Merck Group (3 mentions) Perk sc 7383 and sc 16982 r, by Santa Cruz Biotechnology (2 mentions) Etv5 wh0002119m2, by Merck Group (2 mentions) Gfp sc 9996, by Santa Cruz Biotechnology (2 mentions) α tubulin 2144, by Cell Signaling Technology (2 mentions) Ha c29f4, by Cell Signaling Technology (2 mentions) Myc tag 71d10, by Cell Signaling Technology (1 mentions) Etv1 ab81086, by Abcam (1 mentions)

Close spelling variants of this product

M5670 (10 citations)

3 protocols using polyclonal erk m5670

Recombinant Protein Characterization Protocol

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Purified recombinant GST-H-Ras(WT) was obtained from Jena Bioscience. Active recombinant human His-c-Src kinase and GST-SHP2 were obtained from GenWay Biotechnology. Rabbit polyclonal antibodies against Src, phosphorylated (p)Src(Y416), pAKT, AKT, glutathione S-transferase (GST), pSHP2(Y542), SHP2 and HA (polyclonal) were obtained from Cell Signaling Technologies. Polyclonal IgG (sc-2027), pERK (sc-7383), N-Ras (sc-519), and c-Raf (sc-7267) were obtained from Santa Cruz Biotechnology. p-c-Raf(Y340) (#44506G) was obtained from Invitrogen. GFAP and Ki67 were obtained from Dako. Monoclonal antibodies against HA (12CA5) and pTyr (4G10) (05-321) were obtained from Boehringer Ingelheim and Millipore, respectively. Monoclonal FLAG-M2 (F1804), β-actin (A5316) (1:10,000), vinculin (V9264) (1:10,000) and polyclonal ERK (M5670) antibodies (1:5,000) were obtained from Sigma. All antibodies were utilized at a 1:1,000 dilution unless otherwise specified.

Bunda S., Burrell K., Heir P., Zeng L., Alamsahebpour A., Kano Y., Raught B., Zhang Z.Y., Zadeh G, & Ohh M. (2015). Inhibition of SHP2-mediated dephosphorylation of Ras suppresses oncogenesis. Nature Communications, 6, 8859.

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Antibody Source and Dilution Protocol

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The following antibodies were obtained from Cell Signaling Technologies: HA (C29F4) (1:6000), Lamin(A/C) (2032), pERK (4370), ubiquitin (3933), PJA2 (40180), and β-TrCP (D13F10), β-actin (8H10D10) (1:20,000), myc tag (71D10), and α-tubulin (2144) (1:5000). pERK (sc-7383 and sc-16982-R), PJA1 (sc-517068), and GFP (sc-9996) (1:6000) were obtained from Santa Cruz Biotechnology. Ki67 was obtained from Dako. Phospho Ser/Thr (ab17464), T7 tag (ab9138), capicua (ab123822), ETV1 (ab81086), Renilla (ab187338), and myc protein (ab3207) were obtained from Abcam. The following antibodies were purchased from Millipore T7 tag (69522), capicua (ABN446) and capicua (MABN449). FLAG-M2 (F1804), β-actin (A5316) (1:10,000), vinculin (V9264) (1:30,000), ETV5 (WH0002119M2), and polyclonal ERK (M5670) antibodies (1:5000) were obtained from Sigma. PJA1 (MBS153701) was purchased from MyBioSource.com. All antibodies were utilized at a 1:1,000 dilution unless otherwise specified.

Bunda S., Heir P., Metcalf J., Li A.S., Agnihotri S., Pusch S., Yasin M., Li M., Burrell K., Mansouri S., Singh O., Wilson M., Alamsahebpour A., Nejad R., Choi B., Kim D., von Deimling A., Zadeh G, & Aldape K. (2019). CIC protein instability contributes to tumorigenesis in glioblastoma. Nature Communications, 10, 661.

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Antibody Characterization for Cell Signaling

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The following antibodies were obtained from Cell Signaling Technology: HA (C29F4) (1:6,000), c-Src (2108), pSrc(416) (2101), Lamin(A/C) (2032), pERK (4370), β-actin (8H10D10) (1:20,000), and α-tubulin (2144) (1:5,000). pERK (sc-7383 and sc-16982-R) and GFP (sc-9996) (1:6,000) were obtained from Santa Cruz Biotechnology. Phospho Ser/Thr (ab17464), capicua (ab123822), and ETV1 (ab81086) were obtained from Abcam. The following antibodies were purchased from Millipore: capicua (ABN446), capicua (MABN449), EGFR, and pTYR. FLAG-M2 (F1804), β-actin (A5316) (1:10,000), vinculin (V9264) (1:30,000), ETV5 (WH0002119M2), and polyclonal ERK (M5670) antibodies (1:5,000) were obtained from Sigma. All antibodies were utilized at a 1:1,000 dilution unless otherwise specified.

Bunda S., Heir P., Li A.S., Mamatjan Y., Zadeh G, & Aldape K. (2020). c-Src Phosphorylates and Inhibits the Function of the CIC Tumor Suppressor Protein. Molecular cancer research : MCR, 18(5), 774-786.

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