Log In Sign up
The largest database of trusted experimental protocols

Caakt1

Manufactured by Addgene
Visit Supplier

CaAKT1 is a protein-coding gene that encodes a serine/threonine-protein kinase involved in various cellular processes. It plays a role in regulating cell growth, proliferation, and survival.

Automatically generated - may contain errors

Lab products found in correlation

Scramble shrna, by Addgene (1 mentions) Mapple lamp1, by Addgene (1 mentions) Pcdna3, by Thermo Fisher Scientific (1 mentions)

2 protocols using caakt1

1

Lentiviral Constructs of caPAK5 and kdPAK5

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Lentiviral constructs of caPAK5 and kdPAK5, which contain only the coding sequence (CDS) of the mRNAs, were generated by PCR and cloned into the entry vector pDONR221, followed by transferring into destination lentiviral vectors pHAGE-CMV-n-HA-FLAG as previously reported 9 (link). DsRed-Mito and GFP-Mito were gifts from R. Youle (NINDS, NIH). GO-ATeam2 and GO-ATeam3 were gifts from H. Imamura (Kyoto University, Japan). PAK5-shRNAs and Miro-1-shRNA were purchased from Dharmacon. Scramble shRNA, mApple-LAMP-1, caPAK1, caPAK5, kdPAK5, and caAKT1 constructs were purchased from Addgene. SNPH truncated mutants were generated in a previous study.19 (link)
Huang N., Li S., Xie Y., Han Q., Xu X.M, & Sheng Z.H. (2021). Reprogramming an energetic AKT-PAK5 axis boosts axon energy supply and facilitates neuron survival and regeneration after injury and ischemia. Current biology : CB, 31(14), 3098-3114.e7.
+ Open protocol
+ Expand
2

Plasmid Construction for Akt1 and Variants

Check if the same lab product or an alternative is used in the 5 most similar protocols
Plasmids for wild‐type Akt1 (WT‐AKT1) and constitutively active Akt1 (CA‐AKT1) were purchased from Addgene. The cDNAs were subcloned into pcDNA3.1(+) (Thermo Fisher Scientific) with four copies of hemaglutinin (HA) tags. The cDNAs encoding Jade1 lacking two PHD domains and Siah1 lacking the ring domain were amplified by PCR from HepG2 cells randomly primed cDNA libraries and then subcloned into pcDNA3.1‐HA.
Liu S., Wang J., Shao T., Song P., Kong Q., Hua H., Luo T, & Jiang Y. (2017). The natural agent rhein induces β‐catenin degradation and tumour growth arrest. Journal of Cellular and Molecular Medicine, 22(1), 589-599.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!