ROS levels were assessed using a dihydroethidium staining kit (DHE, Thermo Fisher, Rockford, IL, United States) according to the manufacturer’s instructions. 5*106 THP-1 cells were induced to differentiate into macrophages as described above. Then, cells were washed with PBS and stained with10 μM DCFH-DA for 30 min at 37°C under dark conditions. The level of ROS was determined by FACSCCalibur flow cytometer (BD Biosciences, United States).
Facsc calibur flow cytometer
Manufactured by BD
Sourced in United States
The BD FACSCalibur flow cytometer is a compact and versatile instrument designed for multicolor flow cytometric analysis. It features a blue argon-ion laser and an optional red diode laser to detect a range of fluorescent markers. The instrument collects data on up to four fluorescent parameters and forward and side scatter characteristics of individual cells or particles.
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Lab products found in correlation
3 protocols using facsc calibur flow cytometer
1
Measuring Oxidative Stress in Macrophages
2
DHA Enhances Anti-Angiogenic Signaling
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DHA (Beijing Zhongsha Jinqiao Biotechnology Co., Ltd); SSeCKS siRNA (Santa Cruz, CA, USA); ELISA kit and TUNEL kit (Jiancheng, Nanjing, China); Perkin Elmer Microplate reader (PerkinElmer Victor 1420, USA); Ang-1, Ang-2, and VEGF mouse anti-human monoclonal antibodies (R&D Systems, Minneapolis, USA); SSeCKS rabbit anti-human monoclonal antibodies (Santa Cruz, CA, USA); Goat anti-mouse polyclonal antibodies and mouse anti-rabbit polyclonal antibodies (R&D Systems, Minneapolis, USA); Odyssey color infrared laser scan-imaging instrument (Li-Cor, USA). FACSC calibur flow cytometer (BD, New Jersey, USA).
3
ROS Measurement by DCFH-DA Assay
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The ROS levels were measured by a ROS Assay with DCFH‐DA (Beijing Solarbio). After treatment with drugs for 8 hours, cells were washed with PBS and stained with10 µM DCFH‐DA for 30 minutes at 37°C under dark conditions. The level of ROS was determined by FACSC calibur flow cytometer (BD Biosciences, USA).
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