Desmin

Manufactured by Leica

Sourced in Switzerland

Desmin is a structural protein found in muscle cells. It plays a crucial role in maintaining the integrity and organization of the cytoskeleton within muscle cells. Desmin is commonly used as a biomarker in immunohistochemical and immunofluorescent applications to identify and characterize muscle tissue and muscle-related disorders.

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Lab products found in correlation

Dysferlin, by Merck Group (4 mentions) Myogenin, by Agilent Technologies (1 mentions) Synaptophysin, by Leica (1 mentions) Chromogranin a, by Roche (1 mentions) Ascl1, by BD (1 mentions) Anti f4 80, by Thermo Fisher Scientific (1 mentions) Alexa 488 or 594, by Thermo Fisher Scientific (1 mentions) Anti rab13, by Affinity Biosciences (1 mentions) Anti desmin, by Abcam (1 mentions) Anti ctbp2, by BD (1 mentions) Myogenin, by Cell Marque (1 mentions) H3k27me3, by Merck Group (1 mentions) Alpha actinin, by Merck Group (1 mentions) Alpha smooth muscle actin, by Merck Group (1 mentions) Tra 1 60, by Merck Group (1 mentions) Tra 1 81, by Merck Group (1 mentions) Ssea3, by R&D Systems (1 mentions) Glial fibrillary acidic protein (gfap), by Agilent Technologies (1 mentions) Nanog, by Abcam (1 mentions) Quantitative image analysis, by Leica (1 mentions)

9 protocols using desmin

Quantitative IHC Analysis of GFAP, Desmin, and Caspase-3

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Immunohistochemistry (IHC) of all sections was done using the avidin–biotin–peroxidase method using the Bond‐Max fully automated immunostainer (Leica Biosystems, Deer Park, IL, USA) (Hsu et al., 1981 (link)). GFAP (ready‐to‐use primary antibody, mouse anti‐human, monoclonal antibody, PA0026; Leica Biosystems) and desmin (ready to use primary antibody, mouse anti‐human, monoclonal antibody, PA0032; Leica Biosystems) were used to stain the hippocampus and renal glomerular and interstitial myofibroblasts, respectively. The antibodies were added to each section. The quantification of the IHC was done for each slide (five microscopic fields for GFAP and 20 microscopic fields for desmin; magnification was 50 μm as present on scale, quantification was done in each microscopic field as a whole), using quantitative‐image analysis (Leica Microsystems). The quantification of desmin in the glomeruli and interstitium was done using quantitative‐image analysis (Leica Microsystems).
Similarly, quantification of apoptotic cells in hippocampus required the addition of an apoptotic marker Anti‐Caspase‐3 antibody, monoclonal antibody (ab184787) which was quantified in five microscopic fields (magnification was 50 μm as present on scale, quantification was done in each microscopic field as a whole) using the quantitative‐image analysis (Leica micro‐systems, Switzerland).

Basta M., Yassin H.A., Aly R.G, & El Sayed N.S. (2022). Possible protective effect of zinc administration on renal and cognitive changes occurring in uninephrectomized adult male Wistar rats. Experimental Physiology, 108(2), 253-267.

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ASCL1 Expression in Rhabdomyosarcomas

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Tumors were immunostained for desmin (Leica, Novocastra, Buffalo Grove, IL, clone DE-R-11, 1:50 – 1:100), myogenin (Dako, Carpinteria, CA, clone F5D, 1:25 – 1:50), MyoD1 (Ventana, Tucson, AZ, clone EP212, 1μg/mL), synaptophysin (Leica, Novocastra, Buffalo Grove, IL, clone 27G12, 1:100 – 1:200), chromogranin A (Ventana, Tucson, AZ, clone LK2H10, 1μg/mL), CD56 (Dako, Carpinteria, CA, clone 123C3, 1:50 – 1:100), TTF1 (Leica, Novocastra, Buffalo Grove, IL, clone SPT24, 1:200) and ASCL1 (BD Pharmingen, San Jose, CA, Clone: 24B72D11.1, 1:100). Immunohistochemical results were dichotomized into cases with absent expression and those with positive expression, defined by staining noted in at least 5% of neoplastic cells. Immunophenotyping of all RMS was performed on representative whole slide sections.
ASCL1 expression in rhabdomyosarcomas was compared to 70 patients with HGNEC and 110 patients with typical urothelial carcinomas (UC) from specimens obtained from patients treated with radical cystectomy at our institution between 1987 and 2014 (18 (link)). Tissue microarrays, with four 1.0mm cores representing each HGNEC and typical UC, were immunostained for ASCL1 for this purpose. In addition, whole slide sections of 47 cases of non-urinary bladder rhabdomyosarcomas were immunostained for ASCL1, to assess specificity.

Gupta S., Sosa C.P., Kosari F., Folpe A., Bhinge K.N., Yang L., Agahi A., Johnson S.H., Frank I., Boorjian S.A., Hansel D.E., Al-Ahmadie H.A., Reuter V.E., Vasmatzis G., Jimenez R.E., Herrera-Hernandez L, & Cheville J.C. (2019). A Comparison of Adult Rhabdomyosarcoma and High Grade Neuroendocrine Carcinoma of the Urinary Bladder Reveals Novel PPP1R12A Fusions in Rhabdomyosarcoma. Human pathology, 88, 48-59.

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Immunolabeling of Cochlear Pericytes

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Cochleae or HEI‐OC1 cells were immediately harvested and fixed by 4% paraformaldehyde (PFA) for 2 h or 15 min at room temperature. SV or the basilar membrane was dissected, blocked with donkey serum (8%), and treated with primary antibodies, anti‐desmin (1:200, Abcam) and anti‐F4/80 (1:200, eBioscience), anti‐CtBP2 (1:200, BD‐Biosciences), anti‐Myosin VIIa (1:200, Proteus Biosciences), anti‐Rab13 (1:200, Affinity) and secondary antibodies against rabbit or rat labeled with Alexa 488 or 594 (1:500, Thermo Fisher Scientific). Isolectin GS‐IB4 conjugated to Alexa‐Fluor 647 (1:100, Thermo Fisher Scientific) was applied for 1 h at room temperature. After mounting and capturing pictures by a laser scanning confocal microscope (Leica, SP8), the coverage of pericytes or PVM/Ms, which was displayed as desmin or F4/80 area/Isolectin area, was quantified by ImageJ.
⁵ (link)

Wang X., Gu J., Xu K., Xu B., Yu D, & Wu H. (2023). Sound conditioning strategy promoting paracellular permeability of the blood‐labyrinth‐barrier benefits inner ear drug delivery. Bioengineering & Translational Medicine, 9(1), e10596.

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Microscopic Examination of Sarcoma

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Microscopic examination of hematoxylin and eosin-stained slides was conducted in all cases. For a subset of cases, immunohistochemical stains for desmin (Leica, Buffalo Grove, IL), myogenin (Cell Marque, Rocklin, CA), H3K27me3 (EMD Millipore, Burlington, MA), alpha-1 antitrypsin (Roche Ventana), and p53 (Leica) were evaluated. Three cases were stained for periodic acid-Schiff (PAS) with diastase digestion.

Kamihara J., Paulson V., Breen M.A., Laetsch T.W., Rakheja D., Shulman D.S., Schoettler M.L., Clinton C.M., Ward A., Reidy D., Pinches R.S., Weiser D.A., Mullen E.A., Schienda J., Meyers P.A., DuBois S.G., Nowak J.A., Foulkes W.D., Schultz K.A.P., Janeway K.A., Vargas S.O, & Church A.J. (2020). DICER1-associated central nervous system sarcoma in children: comprehensive clinicopathologic and genetic analysis of a newly described rare tumor. Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc, 33(10).

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Pluripotency and Lineage Marker Detection

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Cells were fixed in 4% paraformaldehyde for 10 min, permeabilized with 0.5% Tween-20 in PBS, and incubated to 0.1% Tween-20 with 10% horse serum. We exposed the cells to primary antibodies overnight and to secondary antibodies for 1 hour (Alexa Fluor, Invitrogen). We employed the following primary antibodies: SSEA-3 (1:100, R&D), SSEA-4 (1:500, DSHB), TRA1-60 (1:500, Chemicon), TRA1-81 (1:500, Chemicon), NANOG (1:500 Abcam), alpha-fetoprotein (1:500, DAKO), alpha-actinin (1:500 Sigma), alpha smooth muscle actin (1:500, Sigma), Desmin (1:100, Novocastra), TuJ1 (1:1000, Sigma), and glial fibrillary acidic protein (GFAP, 1:1000, DAKO; 1:500, Sigma). Alkaline phosphatase staining was performed20 (link). For the analysis, we used a confocal LEICA LCS2 microscope.

Nizzardo M., Simone C., Dametti S., Salani S., Ulzi G., Pagliarani S., Rizzo F., Frattini E., Pagani F., Bresolin N., Comi G, & Corti S. (2015). Spinal muscular atrophy phenotype is ameliorated in human motor neurons by SMN increase via different novel RNA therapeutic approaches. Scientific Reports, 5, 11746.

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Muscle Biopsy Staining Protocol

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Four patients received muscle biopsies. Muscle specimens were obtained from biceps in three patients, and from tibialis anterior in one patient. The muscle specimens were frozen in isopentane, and cooled in liquid nitrogen. Serial frozen sections were stained by routine histological and histochemical methods and by standard immunohistochemical techniques for dystrophin (N-terminus, C-terminus, and rod domain, Novocastra), sarcoglycan complex, (α-, β-, and γ-, Novocastra), dysferlin (Chemicon), and desmin (Novocastra).

Yu M., Zhu Y., Lu Y., Lv H., Zhang W., Yuan Y, & Wang Z. (2020). Clinical features and genotypes of Laing distal myopathy in a group of Chinese patients, with in-frame deletions of MYH7 as common mutations. Orphanet Journal of Rare Diseases, 15, 344.

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Comprehensive Muscle Biopsy Analysis

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We performed muscle biopsy for all of the patients. Serial frozen sections were stained by routine histological and histochemical methods and by immunohistochemical methods for dystrophin (N-terminus, C-terminus, and rod domain, Novocastra), sarcoglycan complex, (α-, β-, and γ-, Novocastra), dysferlin (Chemicon), and desmin (Novocastra).

Yu M., Zheng Y., Jin S., Gang Q., Wang Q., Yu P., Lv H., Zhang W., Yuan Y, & Wang Z. (2017). Mutational spectrum of Chinese LGMD patients by targeted next-generation sequencing. PLoS ONE, 12(4), e0175343.

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Muscle Biopsy Immunohistochemistry Analysis

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Four patients received muscle biopsies. Muscle specimens were obtained from biceps in three patients, and from tibialis anterior in one patient. The muscle specimens were frozen in isopentane, cooled in liquid nitrogen. Serial frozen sections were stained by routine histological and histochemical methods and by standard immunohistochemical techniques for dystrophin (N-terminus, C-terminus, and rod domain, Novocastra), sarcoglycan complex, (α-, β-, and γ-, Novocastra), dysferlin (Chemicon), and desmin (Novocastra).

Yu M., Zhu Y., Lu Y., Lv H., Zhang Q., Yuan Y., & Wang Z. (2020). Clinical Features and Genotypes of Laing Distal Myopathy in a Group of Chinese Patients, With In-Frame Deletions of MYH7 as Common Mutations.

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Muscle Biopsy and Protein Analysis

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Yu M., Zhu Y., Lu Y., Lv H., Zhang Q., Yuan Y., & Wang Z. (2020). Clinical features and genotypes of Laing distal myopathy in a group of Chinese patients, with in-frame deletions of MYH7 as common mutations.

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