Cefuroxime axetil was provided by Meilun (Dalian, Liaoning, China). Polylactic acid (PLA) (Mw = 17 kDa) was obtained from SunLip Biotechnology (Shanghai, China). PLA-HPG was synthesized as previously described (Deng et al., 2014 (link); Deng et al., 2015 (link)). Cyanine 5 (Cy5) and cyanine 7.5 (Cy7.5) were ordered from Aladdin (Shanghai, China). NaIO4, Na2SO3, anhydrous dichloromethane (DCM), sodium dodecyl sulfate (SDS), and HPLC-grade methanol were obtained from Macklin (Shanghai, China). N, N′-diisopropylcarbodiimide (DIC) was purchased from Sigma-Aldrich (St. Louis, MO, United States). Potassium dihydrogen phosphate and potassium ammonium dihydrogen phosphate were chemical reagents (Guangzhou, Guangdong, China).
Hplc grade methanol
Manufactured by Maclin Biochemical Technology
Sourced in China
HPLC-grade methanol is a high-purity solvent used in high-performance liquid chromatography (HPLC) applications. It is a colorless, volatile liquid with a characteristic alcohol odor. HPLC-grade methanol meets strict specifications for low levels of impurities, ensuring consistent performance and reliable results in HPLC analysis.
Automatically generated - may contain errors
Lab products found in correlation
Acetonitrile, by Maclin Biochemical Technology (4 mentions)
Sodium dodecyl sulfate (sds), by Maclin Biochemical Technology (1 mentions)
N n diisopropylcarbodiimide, by Merck Group (1 mentions)
Na2so3, by Maclin Biochemical Technology (1 mentions)
Acetic acid, by Maclin Biochemical Technology (1 mentions)
Acetone, by Maclin Biochemical Technology (1 mentions)
Ace from rabbit lung, by Merck Group (1 mentions)
Hippuryl histidyl leucine, by Merck Group (1 mentions)
Potassium ferricyanide, by Maclin Biochemical Technology (1 mentions)
Caffeic acid, by Maclin Biochemical Technology (1 mentions)
Ferric chloride, by Maclin Biochemical Technology (1 mentions)
Sinapic acid, by Maclin Biochemical Technology (1 mentions)
Ascorbic acid, by Maclin Biochemical Technology (1 mentions)
Trichloroacetic acid, by Maclin Biochemical Technology (1 mentions)
Potassium persulfate, by Maclin Biochemical Technology (1 mentions)
Quercetin, by Maclin Biochemical Technology (1 mentions)
2 2 diphenyl 1 picrylhydrazyl (dpph), by Yuanye Bio-Technology (1 mentions)
2 2 azinobis 3 ethylbenzothiazoline 6 sulfonic acid (abts), by Yuanye Bio-Technology (1 mentions)
Phosphoric acid, by Shanghai Titan Scientific (1 mentions)
Absolute ethanol, by Shanghai Titan Scientific (1 mentions)
7 protocols using hplc grade methanol
1
Cefuroxime axetil-loaded PLA-HPG nanoparticles
2
Quantitative Analysis of Pesticide Residues
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
ABM (97%), acetamiprid (99%), eprinomectin (91%), HPLC-grade methanol, acetonitrile, acetic acid, and acetone were obtained from Macklin Biochemical Technology Co., Ltd. (Shanghai, China). HMDI (>98%) and 1-butyl-3-methylimidazolium tetrafluoroborate ([BMIM]BF4) (>98%) were acquired from TCI (Shanghai, China). β-CD (>98%) was provided by Sinopharm Chemical Reagent Co. Ltd. (Beijing, China), and before utilization, it was dried for 24 h at 110 °C. Dimethyl sulfoxide (DMSO) (Superdry) (≥99.9%) was provided by J&K Tech. Ltd. (Beijing, China). Fruits samples were purchased from the local market in Nanning, Guangxi (China). All the reagents used were of analytical grade.
3
Isolation and Characterization of Crude ACE
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Crude ACE was isolated according to the reported literature [44 (link)]. Hippuryl-histidyl-leucine (HHL; ≥99%) and ACE (from rabbit lung) were purchased from Sigma Chemical Co. (St. Louis, MO, USA). 2-MeIM (≥99%), DA (≥99%) and HPLC-grade trifluoroacetic acid were purchased from Shanghai Aladdin Biochemical Technology Co., Ltd. (Shanghai, China). DA-PEGx-DA (x = 2000, 5000 and 10,000) were purchased from Guangzhou Tansh-tech Co., Ltd. (Guangzhou, China). VPP and GF-6 were obtained from GL Biochem (Shanghai) Ltd. (Shanghai, China). HPLC-grade methanol and acetonitrile were purchased from Shanghai Macklin Biochemical Co., Ltd. (Shanghai, China). All the other reagents were of analytical grade and purchased from XiLong Science (Guangdong, China).
4
Antioxidant Activities of Natural Polyphenols
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Six natural polyphenols (quercetin, caffeic acid, sinapic acid, catechin, ferulic acid, 3,4-dihydroxybenzoic acid), ascorbic acid, HPLC-grade methanol, as well as potassium persulfate, trichloroacetic acid, ferric chloride, and potassium ferricyanide were obtained from Shanghai Macklin Biochemical Co., Ltd. (Shanghai, China). While DPPH, ABTS, and SAC were supplied by Shanghai yuanye Bio-Technology Co., Ltd. (Shanghai, China).
5
HPLC Quantification of CNF2 in Biological Samples
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Apparatus Shimadzu LC-20AT (Shimadzu, Japan) HPLC; TGL-15B high-speed desktop centrifuge (Shanghai Anting Scientific Instrument Factory, China); Bullet Blender rapid tissue cell disruption instrument (Next Advance, U.S.A.); PHS-3C PH meter (INESA, China); GL224-1SCN analytical electronic balance (one per ten thousand, Sartorius, China); XH-C vortex mixer (Jintan Chengdong Xinrui Instrument Factory, China); KQ-500B ultrasonic cleaner (Kunshan Ultrasonic Instrument Co., Ltd., China).
Chemicals CNF2 (purity: 95%) was prepared by our laboratory. Ofloxacin standard (Internal standard (IS)) (Lot: HY19S919, purity ≥ 98.0%) was purchased from Shanghai Hengyuan Biotechnology Co., Ltd. Heparin sodium (batch number: P1356787, USP), sodium chloride, triethylamine, phosphoric acid, diethyl ether and absolute ethanol were analytical reagent, all purchased from Shanghai Titan Technology Co., Ltd. HPLC grade methanol and acetonitrile were purchased from Shanghai Macklin Biochemical Co., Ltd. Ultrapure water is prepared by UPT-II-20T ULUPURE ultrapure water machine (Baoshan District Urban Industrial Park, Shanghai, China).
Chemicals CNF2 (purity: 95%) was prepared by our laboratory. Ofloxacin standard (Internal standard (IS)) (Lot: HY19S919, purity ≥ 98.0%) was purchased from Shanghai Hengyuan Biotechnology Co., Ltd. Heparin sodium (batch number: P1356787, USP), sodium chloride, triethylamine, phosphoric acid, diethyl ether and absolute ethanol were analytical reagent, all purchased from Shanghai Titan Technology Co., Ltd. HPLC grade methanol and acetonitrile were purchased from Shanghai Macklin Biochemical Co., Ltd. Ultrapure water is prepared by UPT-II-20T ULUPURE ultrapure water machine (Baoshan District Urban Industrial Park, Shanghai, China).
6
Isolation and Characterization of Bioactive Compounds
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
ChCl, aminoethyl alcohol, D-glucose, glycerol, oxalic acid, lactic acid, urea, methanol-d4, QE, 20-hydroxyecdysone, sodium nitrite, and aluminium trichloride (AlCl3) were purchased from Aladdin Industrial Co., Ltd. (Shanghai, China). HPLC-grade methanol and acetonitrile (ACN) were purchased from Shanghai Macklin Biochemical Co., Ltd. (Shanghai, China). Macroporous resins (D101, HPD 600, NKA-9, HPD 400, and AB-8) and analytical-grade methanol were purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). Deionized water was obtained using a Unique-R20 purification system (Xiamen RSJ Scientific Instruments Co., Ltd., Xiamen, China).
Nuclear magnetic resonance (NMR) spectra were recorded on an Agilent DD2 spectrometer (500 MHz for 1H and 125 MHz for 13C NMR; Agilent, CA, USA). High-resolution electrospray ionization–tandem mass spectrometry (HRESI-MS) was performed using an LTQ Orbitrap XL spectrometer (Thermo Scientific, MA, USA).
Nuclear magnetic resonance (NMR) spectra were recorded on an Agilent DD2 spectrometer (500 MHz for 1H and 125 MHz for 13C NMR; Agilent, CA, USA). High-resolution electrospray ionization–tandem mass spectrometry (HRESI-MS) was performed using an LTQ Orbitrap XL spectrometer (Thermo Scientific, MA, USA).
7
Quantification of Lutein in Samples
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
A lutein standard (HPLC grade ≥95%) was purchased from the Sigma-Aldrich Chemical Co. (St. Louis, USA) and kept in darkness at 4℃. HPLC grade methanol, acetonitrile and n-hexane, and butylated hydroxytoluene (BHT, ≥ 99% FG), and acetone (GR grade) were purchased from the Shanghai Macklin Biochemical Co. HPLC-grade deionized water was sourced with a Milli-Q 50 system (Millipore Iberica S.A., Madrid, Spain). A stock solution of the lutein standard was dissolved in dicholoromethane, diluted to 0.1 mg/mL with methanol, and stored at -80℃. Lutein standards at 1.0, 1.25, 2.5, 5.0 and 10.0 μg/mL were used to generate a regression equation for quantification of test samples.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!