Colorimetric ache assay kit

The activity of AChE was carried out using a colorimetric AChE assay kit from Abcam (Cambridge, UK, a138871). First, 0.2 U/mL of AChE and the reaction mixture were applied to each well of the 96-well microplate containing the standard and test samples. Next, the plate was protected from light exposure and incubated at room temperature for 30 min. The absorbance was scanned with a microplate reader at 410 ± 5 nm (Berthold Technologies, Bad Wildbad, Germany). Corrections were made to the measured values based on the absorbance of the reaction mixture and test samples, and the AChE activity rate was calculated as a percentage.

AChE activity was measured according to Ellman’s method [24 (link)]. Diaphragm homogenates were incubated with 10⁻⁵ M of the butyryl cholinesterase inhibitor, tetraisopropyl pyrophosphoramide iso-OMPA (Sigma Aldrich, Saint Quentin Fallavier, France). A colorimetric AChE assay kit was used to measure the AChE activity according to manufacturer’s instructions (Abcam, Cambridge, UK) and the data were expressed in μmol/min/mU.