HAuCl4·3H2O (99.9% pure), H2PtCl6·6H2O (99% pure), L-(+)-ascorbic acid (99% pure), 3,3′-diaminobenzidine (99% pure), o-phenylenediamine (98% pure) and carbamoyl-2,2,5,5-tetramethyl-3-pyrrolin-1-yloxy (CTPO, 99% pure) were provided by Alfa Aesar. 5,5-Dimethyl-1-pyrroline N-oxide (DMPO, 98% pure) and 2,2,6,6-tetramethylpiperidine (TEMP, 99% pure) were acquired from Innochem. Rhodamine B and glucose oxidase (GOx) were supplied by Amresco. Reduced cytochrome C was purchased from Abcam (Shanghai, China). 2,2′-Azinobis (3-ethylbenzo-thiazoline-6-sulfonic acid) diammonium salt (99% pure) was bought from J&K Scientific Ltd. (Beijing, China). TMB (99% pure) was obtained from Acros. Trisodium citrate dihydrate (99% pure) was provided by Sinopharm Chemical Reagent Co., Ltd. (Beijing, China). PBS buffer (pH 7.2–7.4), α-amylase, lysozyme, and horseradish peroxidase (HRP) were provided by Solarbio Science & Technology Co., Ltd. (Beijing, China). Collagen was supplied by Yuanye Co., Ltd. (Shanghai, China). SARS-CoV-2 S1 recombinant protein (S1 protein), nucleocapsid protein (N protein) and anti-SARS-CoV-2 S1 antibody were acquired from Sangon Biotech Co., Ltd. (Shanghai, China).
Glucose oxidase
Manufactured by Avantor
Sourced in United States
Glucose oxidase is an enzyme that catalyzes the oxidation of glucose to gluconic acid and hydrogen peroxide. It is commonly used in laboratory settings for the detection and quantification of glucose levels in various samples.
Automatically generated - may contain errors
Lab products found in correlation
Vectashield, by Vector Laboratories (2 mentions)
Catalase, by Merck Group (2 mentions)
Lysozyme, by Solarbio (1 mentions)
Horseradish peroxidase hrp, by Solarbio (1 mentions)
L ascorbic acid, by Thermo Fisher Scientific (1 mentions)
3 3 diaminobenzidine (dab), by Thermo Fisher Scientific (1 mentions)
Haucl4 3h2o, by Thermo Fisher Scientific (1 mentions)
O phenylenediamine, by Thermo Fisher Scientific (1 mentions)
2 2 azino bis 3 ethylbenzothiazoline 6 sulfonic acid diammoniumsalt, by J&K Scientific (1 mentions)
H2ptcl6 6h2o, by Thermo Fisher Scientific (1 mentions)
Rhodamine b, by Avantor (1 mentions)
3 3 5 5 tetramethylbenzidine (tmb), by Thermo Fisher Scientific (1 mentions)
Pbs buffer, by Solarbio (1 mentions)
α amylase, by Solarbio (1 mentions)
Trisodium citrate dihydrate, by Sinopharm (1 mentions)
Apo tirf 100 oil objective, by Nikon (1 mentions)
Sodium phosphate dibasic dodecahydrate, by Merck Group (1 mentions)
1 ethyl 3 3 dimethylaminopropyl carbodiimide edc, by Merck Group (1 mentions)
2 n morpholino ethanesulphonic acid mes, by Merck Group (1 mentions)
Hydrogen tetrachloroaurate 3 trihydrate, by Merck Group (1 mentions)
5 protocols using glucose oxidase
1
Synthesis and Characterization of COVID-19 Proteins
2
Super-resolution Imaging of Retina Sections
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Immediately prior to imaging, 10% sodium hydroxide (w/v) was mixed with pure 200-proof ethanol for 45 minutes to prepare a mild sodium ethoxide solution. Glass-bottom dishes with ultrathin retina sections were immersed for 30–45 minutes for chemical etching of the resin. Etched sections were then washed and dried on a 50°C heat block. The following STORM imaging buffer was prepared: 45 mM Tris (pH 8.0), 9 mM NaCl, and oxygen scavenging system: 0.7 mg/mL glucose oxidase (Amresco) + 42.5 μg/mL catalase (MilliporeSigma), 10% (w/v) glucose + 100 mM MEA (i.e., l -cysteamine, Chem-Impex) + 10% VECTASHIELD (Vector Laboratories). Imaging buffer was added onto the dried, etched sections and sealed with a second number 1.5 coverslip for imaging.
Imaging was performed on the Nikon N-STORM system, which features a CFI Apo TIRF 100× oil objective (NA1.49) on an inverted Nikon Ti Eclipse microscope. STORM image acquisition was controlled by NIS-Elements Ar software.
To begin a STORM acquisition, both the 561 nm and 647 nm laser lines were increased to maximum power to photobleach the fluorescence and initiate photoswitching. Imaging frames were collected at approximately 56 frames per second. A total of 50,000 frames were collected for each imaging experiment.
Imaging was performed on the Nikon N-STORM system, which features a CFI Apo TIRF 100× oil objective (NA1.49) on an inverted Nikon Ti Eclipse microscope. STORM image acquisition was controlled by NIS-Elements Ar software.
To begin a STORM acquisition, both the 561 nm and 647 nm laser lines were increased to maximum power to photobleach the fluorescence and initiate photoswitching. Imaging frames were collected at approximately 56 frames per second. A total of 50,000 frames were collected for each imaging experiment.
3
Detection of Influenza Virus H3N2 Using Aptamer
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The carboxylated matrix metalloproteinases (MMPs) (1.02 μm, 10 mg ml−1) were obtained from Invitrogen (USA). Sodium phosphate monobasic dihydrate (NaH2PO4·2H2O), sodium phosphate dibasic dodecahydrate (Na2HPO4·12H2O), sodium chloride (NaCl), concanavalin A (ConA) ((type VI, from Canavalia ensiformis (Jack bean)), hydrogen tetrachloroaurate (III) trihydrate (HAuCl4·3H2O), N-hydroxysuccinimide (NHS) and 2-(N-morpholino)ethanesulphonic acid (MES), 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC) were purchased from Sigma (St Louis, MO, USA). Glucose oxidase was obtained from Amresco (USA). Influenza virus (H3N2) was supplied by Huazhong Agricultural University. The selected aptamer with high affinity and specificity to the H3N2 surface protein was developed by Jeon [33 (link)], and DNA oligonucleotides were synthesized and purified by Sangon.
The aptamer A22: 5′-NH2-AATTAACCCTCACTAAAGGGCTGAGTCTCAAAACCGCAATAACTGGTTGTATGGTCGAATAAGTTAA-3′
The aptamers were prepared in 10 mM Tris-HCl buffer (1 mM EDTA, pH = 8.0).
The aptamer A22: 5′-NH2-AATTAACCCTCACTAAAGGGCTGAGTCTCAAAACCGCAATAACTGGTTGTATGGTCGAATAAGTTAA-3′
The aptamers were prepared in 10 mM Tris-HCl buffer (1 mM EDTA, pH = 8.0).
4
Fabrication of Electrochemical Sensors
5
STORM Imaging of Resin-Embedded Sections
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Immediately prior to imaging, 10% sodium hydroxide (w/v) was mixed with pure 200proof ethanol for 45 minutes to prepare a mild sodium ethoxide solution. Glass-bottom dishes with ultra-thin retina sections were immersed for 30-45 minutes for chemical etching of the resin. Etched sections were then washed and dried on a 50°C heat block.
The following STORM imaging buffer was prepared: 45 mM Tris (pH 8.0), 9 mM NaCl, oxygen scavenging system: 0.7 mg•ml-1 glucose oxidase (Amresco) + 42.5 µg ml-1 catalase (Sigma), 10% (w/v) glucose + 100 mM MEA (i.e. L-cysteamine, Chem-Impex) + 10% VECTASHIELD (Vector Laboratories). Imaging buffer was added onto the dried, etched sections and sealed with a second number 1.5 coverslip for imaging.
Imaging was performed on the Nikon N-STORM system, which features a CFI Apo TIRF 100x oil objective (NA1.49) on an inverted Nikon Ti Eclipse microscope. STORM image acquisition was controlled by NIS-Elements Ar software.
To begin a STORM acquisition, both the 561 nm and 647 nm laser lines were increased to maximum power to photobleach the fluorescence and initiate photoswitching.
Imaging frames were collected at ~56 frames per second. 50,000 frames were collected for each imaging experiment.
The following STORM imaging buffer was prepared: 45 mM Tris (pH 8.0), 9 mM NaCl, oxygen scavenging system: 0.7 mg•ml-1 glucose oxidase (Amresco) + 42.5 µg ml-1 catalase (Sigma), 10% (w/v) glucose + 100 mM MEA (i.e. L-cysteamine, Chem-Impex) + 10% VECTASHIELD (Vector Laboratories). Imaging buffer was added onto the dried, etched sections and sealed with a second number 1.5 coverslip for imaging.
Imaging was performed on the Nikon N-STORM system, which features a CFI Apo TIRF 100x oil objective (NA1.49) on an inverted Nikon Ti Eclipse microscope. STORM image acquisition was controlled by NIS-Elements Ar software.
To begin a STORM acquisition, both the 561 nm and 647 nm laser lines were increased to maximum power to photobleach the fluorescence and initiate photoswitching.
Imaging frames were collected at ~56 frames per second. 50,000 frames were collected for each imaging experiment.
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