Terazosin (TZ, purity ≥ 98%) and Dextran Sulfate sodium (DSS, MW~40kDa) were purchased from Aladdin Biotechnology Co., Ltd.(Shanghai, China); 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT) and dimethylsulfoxide (DMSO) were from Solarbio Life Science (Beijing, China); Primary rabbit antibodies against Pgk1, caspase-1, GSDMD, p-AKT (T450), and β-actin were purchased from BOSTER Biological Technology Co., Ltd. (Beijing, China); Antibodies against NF-κB p65 (3033) and p-IKBα (5209) were purchased from Cell Signaling Technology (Beverly, MA, USA).
3 4 5 dimethyl 2 thiazolyl 2 5 diphenyl 2h tetrazolium bromide mtt
Manufactured by Solarbio
Sourced in China
3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) is a chemical compound used as a colorimetric assay for assessing cell metabolic activity. It is a pale yellow tetrazolium salt that is reduced in metabolically active cells to form a dark blue formazan product.
Automatically generated - may contain errors
Lab products found in correlation
4 6 diamidino 2 phenylindole (dapi), by Solarbio (2 mentions)
Reactive oxygen detection kit, by Beyotime (2 mentions)
Mitochondrial membrane potential detection kit, by Beyotime (2 mentions)
Dapi 4 6 diamidino 2 phenylindole, by Solarbio (2 mentions)
Seahorse xf glycolysis stress test kit, by Agilent Technologies (2 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (2 mentions)
Paraformaldehyde (pfa), by Solarbio (2 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions)
β actin, by Boster Bio (1 mentions)
Dimethyl sulfoxide (dmso), by Solarbio (1 mentions)
Nude mice, by Spf Biotechnology (1 mentions)
Cyclin d1 antibody 60186 1 ig, by Proteintech (1 mentions)
Silibinin, by Solarbio (1 mentions)
Mitochondrial membrane potential assay kit with jc 1, by Beyotime (1 mentions)
L glutamine, by Thermo Fisher Scientific (1 mentions)
Neurobasal medium, by Thermo Fisher Scientific (1 mentions)
Goat serum, by Solarbio (1 mentions)
Triton x 100, by Solarbio (1 mentions)
Goat anti rabbit igg h l alexa fluor 488, by Abcam (1 mentions)
Reactive oxygen species assay kit, by Beyotime (1 mentions)
9 protocols using 3 4 5 dimethyl 2 thiazolyl 2 5 diphenyl 2h tetrazolium bromide mtt
1
Terazosin and Dextran Sulfate Sodium Cytotoxicity
2
Silibinin Inhibits Cancer Cell Growth
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Silibinin was purchased from Solarbio (CAS No.: 22888-70-6; Beijing, China). RPMI-1640 medium was purchased from Thermo Fisher Scientific (Waltham, United States). Fetal bovine serum (FBS) was purchased from Sijiqing (Hangzhou, China). 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) was purchased from Solarbio (CAS No.: 298-93-1). The TMEM16A antibody (ab53212), MEK1/2 antibody (ab178876), β-catenin antibody (ab223075), and goat antirabbit (IgG) secondary antibody (ab150077) were purchased from Abcam (Cambridge, United Kingdom). The MEK1/2 phospho-antibody (11,205) and ERK1/2 phospho-antibody 12,548) were purchased from Signalway Antibody (Texas, United States). The ERK1/2 antibody (K200062 M) was purchased from Solarbio (Beijing, China). The cyclin D1 antibody (60186-1-Ig) was purchased from Proteintech (Chicago, United States). The N-cadherin antibody (A01577-3), E-cadherin antibody (BM4166), and vimentin antibody (PB9395) were purchased from Boster (Beijing, China). The cleaved-caspase 3 antibody (AF7022) and cleaved-caspase 9 antibody (AF5240) were purchased from Affinity Biosciences (Changzhou, China). Nude mice were purchased from SPF Biotechnology (Beijing, China).
3
Amyloid-Beta Induced Cell Injury Assay
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RPMI Medium 1640 basic(1X), fetal bovine serum (FBS), horse serum (HS), neurobasal™ medium, B-27™ supplement (50 × ), glucose solution, L-glutamine (200 mM) were purchased from Gibco® (Thermo Fisher Scientific, NY, United States). Recombinant Human β-Nerve Growth Factor (NGF, 98% pure) was obtained from Peprotech (Cranbury, NJ, United States). Amyloid β-protein fragment 25–35 (Aβ25–35, 97% pure) and 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT), Triton X-100, paraformaldehyde, goat serum and 4-6-diamidino-2-phenylindole (DAPI) were from Beijing Solarbio Science and Technology Co., Ltd. (Beijing, China). A lactate dehydrogenase (LDH) assay kit was purchased from Nanjing Jiancheng Bioengineering Institute (Nanjing, China). The reactive oxygen species (ROS) assay kit and mitochondrial membrane potential (MMP) assay kit with JC-1 were obtained from the Beyotime Institute of Biotechnology (Nanjing, China). The anti-MAP2 (phospho S136) antibody, goat anti-rabbit IgG H&L (Alexa Fluor® 488) were obtained from Abcam (Cambridge, United Kingdom).
4
Cytotoxicity Evaluation of Taxol Analogs
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Human cervical cancer cell line (HeLa) was obtained from Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences. Dulbecco’s Modified Eagle’s Medium (DMEM), fetal bovine serum (FBS), trypsin, and phosphate-buffered solution (PBS, pH 7.4) were all purchased from Hyclone (San Angelo, TX, USA). Taxol and PTX were donated by Beijing ShuangLu Pharmaceutical Co, Ltd. Peptides and fluorescein isothiocyanate (FITC)-labeled peptides were synthesized by GL Biochem (Shanghai, People’s Republic of China). Chlorpromazine (CPZ) was purchased from Heowns Biochemical Technology (Tianjin, People’s Republic of China), nystatin (Nys) from Topscience (Shanghai, People’s Republic of China), 2-deoxy-d -glucose (2-D-G) and dimethyl sulfoxide (DMSO) from Aladdin (Shanghai, People’s Republic of China), sodium azide (NaN3) from Micxy Chemical Co (Cheng Du, People’s Republic of China), paraformaldehyde (PFA), 4′,6-diamidino-2-phenylindole (DAPI), 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT), and heparin from Solarbio (Beijing, People’s Republic of China). Mouse anti-β-tubulin primary antibody and FITC-labeled goat anti-mouse secondary antibody were purchased from Thermo Fisher Scientific (Waltham, MA, USA). Female BALB/c mice were purchased from Beijing HFK Bioscience Co, Ltd (Beijing, People’s Republic of China).
5
Synthesis and Cellular Evaluation of 2-Cl-trityl Chloride Resin
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2-Cl-trityl chloride resin was supplied by Nankai University Resin Co., Ltd. Fmoc-amino acids and O-benzotriazole-N,N,N′,N′-tetramethyluronium-hexafluorophosphate (HBTU) were purchased from GL Biochem (Shanghai, China). PEM and the other starting materials were supplied by Aladdin. Dulbecco’s modified Eagle’s medium (DMEM), fetal bovine serum (FBS) and penicillin/streptomycin were obtained from HyClone. DAPI (4′, 6-diamidino-2-phenylindole) and 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) were purchased from Solarbio (Beijing, China). A mitochondrial membrane potential detection kit and reactive oxygen detection kit were supplied by Beyotime Biotechnology, and a Seahorse XF Glycolysis Stress Test Kit was supplied by Agilent.
6
Synthesis and Characterization of HA Hydrogels
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Sodium Hyaluronate (HA, Mn = 39 K) was purchased from Huaxi Freda Biomedical Co., Ltd. (Jinan, China) Bovine serum albumin (BSA) and fetal bovine serum (FBS) were supplied by Gibco BRL (Gaithersburg, MD, USA). 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT, 98%) was purchased from Solarbio Co., Ltd. (Beijing, China), and trypsin was provided by Invitrogen (Carlsbad, CA, USA). Tris hydrochloride (purity of 98%) was bought from Darui Fine Chemicals Co., Ltd. (Shanghai, China) and used to prepare the Tris buffer. Other solvents with analytical purity were purchased from Fuyu Fine Chemicals Co., Ltd. (Tianjin, China) and used without further purification.
7
Curcumin and Diosgenin Cytotoxicity Evaluation
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zein and soy protein isolate were purchased from Shanghai Macklin Biochemical in China. Cur (purity ≥ 95%) and Dio (purity ≥ 98%) were purchased from Energy Chemical Company in Shanghai (China). Dulbecco-modified Eagle medium (DMEM), fetal bovine serum (FBS) and 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-Htetrazolium bromide (MTT) were purchased from Solarbio Life Sciences Company (Beijing, China). The LogP values in this work can be found in Drugbank database (https://go.drugbank.com/ ) (accessed on 29 June 2023). Other reagents and solvents are standard, all in analytical grade.
8
Evaluating Immune Cell Activation and Metabolism
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Purified anti-mouse CD28 mAb, anti-mouse CD3 mAb, FITC-conjugated anti-mouse CD4 and PE-conjugated anti-mouse CD69 were purchased from BioLegend (San Diego, CA, USA). The Seahorse XF Glycolysis Stress Test Kit and Mitochondrial Stress Test Kit were provided by Agilent Technologies (Palo Alto, Calif.). Roswell Park Memorial Institute (RPMI) 1640, fetal bovine serum (FBS) and penicillin/streptomycin were ordered from Gibco (South Logan, UT, USA). DAPI (4′,6-diamidino-2-phenylindole) and 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2 H-tetrazolium bromide (MTT) were purchased from Solarbio (Beijing, China). Enhanced ATP Assay Kit, Mitochondrial Membrane Potential Detection Kit and Reactive Oxygen Detection Kit were supplied by Beyotime Biotechnology (Shanghai, China).
9
Biodegradable Polymer Scaffolds for Osteoblast Culture
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PCL and PVP, the biodegradable polymers used in electrospinning, were purchased from Shanghai Yuanye Bio-Technology Co. (Shanghai, China). MC powder was from Beijing Aojing Medical Instruments Co. (Beijing, China). CS (12–16 kDa) was from Shandong Aokang Bio-Technology Co. (Dongying, China). Trichloromethane, methyl alcohol, and glacial acetic acid were from Yantai Far Eastern Fine Chemical Co. (Yantai, China). BER was from Macklin Biotechnology Co. (Shanghai, China). The mouse osteoblast cell line MC3T3-E1 (Subclone 14) and α-Minimal Essential Medium used in cell experiments were from Procell Life Science and Technology Co. (Wuhan, China). Fetal bovine serum was from Zhejiang Tianhang Biotechnology Co. (Huzhou, China). Phosphate-buffered saline, penicillin, streptomycin, and 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT) were from Beijing Solarbio Science and Technology Co. (Beijing, China). The Hoechst Staining Kit was from Beyotime Biotechnology Co. (Shanghai, China). The fluorescein isothiocyanate (FITC) Phalloidin Staining Kit was from Shanghai Yeasen Biotechnology Co. (Shanghai, China).
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