Nse assay kit

NaHS was obtained from Sigma Chemical (St. Louis, MO, USA); IPTG (catalogue number: G5042-5G), goat anti-rabbit IgG secondary antibody (catalogue number: G1213-100U), anti-GAPDH antibody (catalogue number: GB12002), marker (catalogue number: G2058-250UL), Coomassie blue R250 (catalogue number: GM1002), LDH assay kit (catalogue number: G1610-100T) were purchased from Servicebio (Wuhan, China); NSE assay kit (catalogue number: MM-0069R2) was obtained from Jiangsu Meimian Industrial, Co., Ltd. (Jiangsu, China); anti-ROCK₂ antibody (catalogue number: ab125025), anti- ROCK₂ (phospho Y722) antibody (catalogue number: ab182648), ATP (catalogue number: ab181719), recombinant Src (catalogue number: ab79635) were purchased from Abcam (San Francisco, CA, USA); 293T cell (catalogue number: c6008) was purchased from Beyotime Biotehchnology, Co. (Shanghai, China).

NaHS was obtained from Sigma Chemical (St. Louis, USA); [γ-³²P] ATP was obtained from China Tongfu Co., Ltd (Beijing, China); PKG1, ATP, DT-2, and ACh were obtained from Sigma-Aldrich (St. Louis, MO, USA); LDH test kit was obtained from Nanjing Jiancheng Biotechnology (Nanjing, China); NSE Assay Kit was obtained from Jiangsu Meimian Industrial, Co., Ltd (Jiangsu); Fluo-8 AM, anti-ROCK₂ (catalog number ab125025), anti-Phospho-RhoA Ser188 (catalog number ab41435), and anti-RhoA (catalog number 187027) were purchased from Abcam (San Francisco, California, USA); and anti-β-actin (catalog number AF7018) and goat anti-rabbit IgG secondary antibody (catalog number S0001) were purchased from Affinity Biosciences (Changzhou, China).

NaHS was obtained from Sigma Chemical (St. Louis, MO, USA). Tris-Base was purchased from Amersco (St. Louis, MO, USA). Ethanol, glacial acetic acid, Na₂S₂O₃, sodium acetate, glutaraldehyde, silver nitrate, formaldehyde, sodium carbonate, and dithiothreitol (DTT) were purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). Cell counting kit-8, ATP, PLK1, PKA, and IPTG were purchased from Abcam (San Francisco, CA, USA). The LDH assay kit was purchased from Servicebio (Wuhan, China). The NSE assay kit was obtained from Jiangsu Meimian Industrial, Co., Ltd. (Jiangsu, China). Flou-8 AM was purchased from biofount (Beijing, China).