DOTAP (890890P), DOPE (850725P), DOPC (850375P), and PE-PEG (880120P) were purchased from Avanti Polar Lipids (Alabaster, Alabama, USA). Tetrachloroauric acid (HAuCl4·3H2O) (520918) and sodium citrate tribasic trihydrate (S4641) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Cell culture media RPMI 1640 (10-040-CV) was obtained from Corning Inc. (Corning, NY, USA). FBS (16000-044) and Penn-Strep (15140-122) were purchased from Life Technologies (Grand Island, NY, USA), Opti-MEM was from Thermo Fisher Scientific (Waltham, MA, USA). The scrambled control siRNA (cat. SIC001), siRNAs against human MICU1 (SASI_Hs01_00070249), CBS (SASI_Hs01_00214623), and CAV1 (SASI_Hs01_00199504) were procured from Sigma-Aldrich. The following primary antibodies were purchased from the specified vendor: rabbit monoclonal anti-MICU1 (#12524, Cell Signaling Technology, Danvers, MA, USA), rabbit polyclonal anti-CBS (#sc-67154, Santa Cruz Biotechnology, Dallas, TX, USA), anti-CAV1 (#SAB871521112, Sigma Aldrich), anti–glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (Sigma-Aldrich), and anti-Ki67 (no. ab833, Abcam).
Sodium citrate tribasic trihydrate s4641
Manufactured by Merck Group
Sourced in United States
Sodium citrate tribasic trihydrate (S4641) is a chemical compound commonly used in laboratory settings. It is a salt of citric acid, with a tribasic structure and three water molecules. This compound is a white crystalline powder that is readily soluble in water. The primary function of sodium citrate tribasic trihydrate is as a buffering agent, maintaining pH levels in various applications.
Automatically generated - may contain errors
Lab products found in correlation
Opti mem, by Thermo Fisher Scientific (4 mentions)
Fbs 16000 044, by Thermo Fisher Scientific (2 mentions)
Anti glyceraldehyde 3 phosphate dehydrogenase gapdh, by Merck Group (1 mentions)
Rabbit polyclonal anti cbs, by Santa Cruz Biotechnology (1 mentions)
Anti cav1, by Merck Group (1 mentions)
Penn strep, by Thermo Fisher Scientific (1 mentions)
Rpmi 1640 10 040 cv, by Corning (1 mentions)
Anti ki67, by Abcam (1 mentions)
Lipofectamine rnaimax transfection reagent, by Thermo Fisher Scientific (1 mentions)
Gold 3 chloride trihydrate 520918, by Merck Group (1 mentions)
2 protocols using sodium citrate tribasic trihydrate s4641
1
Lipid Nanoparticle Synthesis and Characterization
2
Investigating Signaling Pathways in Cell Cultures
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Gold (III) chloride trihydrate (520 918) and sodium citrate tribasic trihydrate (S4641) were bought from Sigma‐Aldrich (St. Louis, MO). Cell culture media RPMI 1640 (10‐040‐CV) was obtained from Corning Inc. (Corning, NY, USA). FBS (16000‐044) and Penn‐Strep (15140‐122) were purchased from Life Technologies (Grand Island, NY, USA), Opti‐MEM was from Thermo Fisher Scientific (Waltham, MA, USA). The following primary antibodies were purchased from the specified vendor: rabbit monoclonal anti‐IGFBP2 (#3922S), ‐PTEN (#9552S), ‐mTOR (#2972S) and ‐AKT S473 (#4058S) (Cell Signaling Technology, Danvers, MA, USA), rabbit anti‐GAPDH (#9545) and ‐tubulin (#T5168) (Sigma Aldrich). Lipofectamine RNAiMAX transfection reagent (13 778 150) was bought from Invitrogen (Waltham, Massachusetts).
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