Caso4.2h2o

Manufactured by Merck Group

Sourced in United States

CaSO4.2H2O is a chemical compound that is commonly known as calcium sulfate dihydrate or gypsum. It is a naturally occurring mineral that is widely used in various industries, including construction, agriculture, and pharmaceuticals. The core function of CaSO4.2H2O is to serve as a source of calcium and sulfate ions, which are essential for various chemical and biological processes.

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CaSO4 (8 citations)

4 protocols using caso4.2h2o

Yeast Growth Media Composition

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The rich medium YPD contained 1% yeast extract, 2% peptone, and 2% glucose, YPD agar (2.5% Bacto agar), and the SD medium contained 6.7 g/L yeast nitrogen base with ammonium sulfate without amino acids (Difco) and 2% glucose. FeSO₄.7H₂O, FeCl₃, CaSO₄.2H₂O_, MgSO₄.7H₂O_, CaCl₂.2H₂O_, MgCl₂.6H₂O, and BPS were purchased from Sigma, and their stock solution was prepared in water. Antimycin A (Sigma, St. Louis, MO, USA), DiOC6(3) (3,3′-Dihexyloxacarbocyanine Iodide) (Invitrogen™, Waltham, MA, USA), and MitoTracker™ Deep Red stock (Invitrogen™) solution was prepared in dimethyl sulfoxide (Sigma). The final concentration of DMSO did not exceed 1% in any assay.

Jing J.L., Ning T.C., Natali F., Eisenhaber F, & Alfatah M. (2022). Iron Supplementation Delays Aging and Extends Cellular Lifespan through Potentiation of Mitochondrial Function. Cells, 11(5), 862.

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Alginate Hydrogel Formation with Borax

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Ultrapure sodium alginate (FMC Biopolymer), was dissolved in 1% (w/v) d-mannitol aqueous solution (Sigma-Aldrich) at a concentration of 1.5% (w/v). Borax was dissolved in different alginate solutions at 0.59 mM or 1.47 mM concentrations. All alginate solutions were next filtered through a 0.22 μm pore Minisart Syringe Filter (Sarto-rius). For gelation, 2.7 mL of the alginate solutions were mixed with 60 μL of 1.22 M CaSO₄·2H₂O (Sigma-Aldrich) through two Luer Lock syringe (BS Syringe) connected with a Fluid Dispensing Connector (Braun). Alginate and CaSO₄·2H₂O were mixed 15 times until complete homogenization. To retard the gelation time, 60 μL of either 0.3 M or 0.5 M Na₂HPO₄·2H₂O (Panreac) were added in the cross-linking reaction.

Ciriza J., Rodríguez-Romano A., Nogueroles I., Gallego-Ferrer G., Cabezuelo R.M., Pedraz J.L, & Rico P. (2021). Borax-loaded injectable alginate hydrogels promote muscle regeneration in vivo after an injury. Materials Science & Engineering. C, Materials for Biological Applications, 123, 112003.

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Aquaporin FO Membrane Characterization

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A commercially available aquaporin FO flat sheet membrane was used in this study. The membrane was a thin film composite with embedded protein in the active layer (Aquaporin A/S, Kongens Lyngby, Denmark). The membrane had a thickness of 110 microns, consisting of a polyamide active layer and polyethersulphone support. NaCl, CaCl₂, Na₂SO₄, and CaSO₄ × 2H₂O were purchased from Sigma–Aldrich, St. Louis, MO, USA. Milli-Q water (Integral 15, Merck, Darmstadt, Germany) was used for the preparation of all solutions.

Omir A., Satayeva A., Chinakulova A., Kamal A., Kim J., Inglezakis V.J, & Arkhangelsky E. (2020). Behaviour of Aquaporin Forward Osmosis Flat Sheet Membranes during the Concentration of Calcium-Containing Liquids. Membranes, 10(5), 108.

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Adsorption of GOn in Natural Ions

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To observe the effect of natural ions on the adsorption of GOn, three ions were tested; phosphates (PO4 3-), sulfates (SO4 2-) and carbonates (CO3 2-). These ions were chosen due to their abundance in natural water in arid and semi-arid regions [21] [22] [23] The concentration range for each ion was selected based on the maximum and minimum concentration reported by Navarro-Noya [32] In the case of PO4 3-the concentrations used were 1.5 and 30 mg/L, whereas SO4 2-were 170 and 1695 mg/L and CO3 2-were 408 and 1293 mg/L [32] . These solutions were prepared by dissolving separately Ca3(PO4)2 (96%, Sigma-Aldrich), CaSO4•2H2O (98%, Sigma-Aldrich) and CaCO3 (99%, Sigma-Aldrich) in deionized water, using an As(III) concentration of 25 mg/L (Sigma-Aldrich As(III) ICP standard solution) and 0.0125 g of GO. For all cases the pH was adjusted to 7. During the adsorption tests the temperature was also kept at 25 °C during 48 h. At the end of the experiment the sample was characterized as described previously.

Reynosa-Martínez A.C., Tovar G.N., Gallegos W.R., Rodríguez-Meléndez H., Torres-Cadena R., Mondragón-Solórzano G., Barroso-Flores J., Alvarez-Lemus M.A., Montalvo V.G, & López-Honorato E. (2020). Effect of the degree of oxidation of graphene oxide on As(III) adsorption. Journal of hazardous materials, 384.

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