Uniformly 15N-labeled NTS-NTSR1 in POPC/POPG (3:2) cNW9 nanodiscs at 200 μM alone and in complex with unlabeled Gαi1β1γ1 at a molar ratio of 5:1 were prepared as described above in NMR buffer (20 mM sodium phosphate (pH 6.9), 50 mM NaCl, 1 mM DTT, 0.5 mM EDTA, 10% D2O). Two-dimensional Transverse Relaxation Optimized Spectroscopy (TROSY) Heteronuclear Single Quantum Coherence (HSQC) were collected with 2000 scans, 200 increments at 45 °C on a Bruker 800-MHz spectrometer equipped with a TXO cryogenic probe. TROSY HSQC measurements were repeated for NTS-NTSR1-cND on an Agilent 700-MHz spectrometer to verify that NTS-NTSR1-cND stays intact after long data acquisition in the magnet at 45 ºC. Data were processed using the NMRPipe software package49 (link).
Txo cryogenic probe
Manufactured by Bruker
The TXO cryogenic probe is a specialized instrument used for nuclear magnetic resonance (NMR) spectroscopy. It is designed to operate at cryogenic temperatures, typically between 4 and 20 Kelvin, in order to enhance the sensitivity and resolution of NMR measurements. The TXO cryogenic probe is capable of detecting and analyzing a wide range of chemical compounds and materials.
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Lab products found in correlation
2 protocols using txo cryogenic probe
1
Structural Dynamics of NTS-NTSR1 in Nanodiscs
2
GPCR Dynamics in Nanodiscs
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Uniformly 15 N-labeled NT-NTR1 in POPC/POPG (3:2) cNW9 nanodiscs at 200 mM alone and in complex with unlabeled Ga i1 b 1 g 1 at a molar ratio of 5:1 were prepared as described above in NMR buffer (20 mM sodium phosphate (pH 6.9), 50 mM NaCl, 1 mM DTT, 0.5 mM EDTA, 10% D 2 O). Two-dimensional Transverse Relaxation Optimized Spectroscopy (TROSY) Heteronuclear Single Quantum Coherence (HSQC) were collected with 2000 scans, 200 increments at 45 °C on a Bruker 800-MHz spectrometer equipped with a TXO cryogenic probe. TROSY HSQC measurements were repeated for NT-NTR1-cND on an Agilent 700-MHz spectrometer to verify that NT-NTR1-cND stays intact after long data acquisition in the magnet at 45 ºC. Data were processed using the NMRPipe software package 45 .
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