Anti polii antibody

Manufactured by Merck Group

The Anti-PolII antibody is a laboratory tool used to detect and study the presence of RNA polymerase II (PolII), a critical enzyme involved in the transcription of genetic information into messenger RNA. This antibody provides a specific and reliable method for identifying and analyzing the distribution and localization of PolII within cellular samples.

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Lab products found in correlation

Anti pgc 1α antibody, by Merck Group (1 mentions) Ez chip kit, by Merck Group (1 mentions) Pureproteome protein a g mix magnetic beads, by Merck Group (1 mentions) Sds page sample loading buffer, by Beyotime (1 mentions) Ecl western blot reagent, by Thermo Fisher Scientific (1 mentions)

2 protocols using anti polii antibody

ChIP-PCR analysis of PRDM16 and PGC-1α

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ChIP was performed on differentiated C3H10-T1/2 cells on day 8 of differentiation using an EZ-ChIP kit in accordance with the manufacturer’s instructions (Millipore). In brief, DNA-containing cell lysates were sheared into fragments of 200–1000 bp by sonication after fixation with formaldehyde. For immunoprecipitation, equal aliquots of cell lysates were incubated with anti-PRDM16 antibody, anti-PGC-1α antibody, anti-PolII antibody (Millipore) or rabbit IgG at 4 °C overnight. Precipitated DNA was amplified by real time PCR in triplicate using the primers listed in Supplementary Table 4. Data were normalized to 18S and expressed as fold of enrichment.

Wu L., Xia M., Duan Y., Zhang L., Jiang H., Hu X., Yan H., Zhang Y., Gu Y., Shi H., Li J., Gao X, & Li J. (2019). Berberine promotes the recruitment and activation of brown adipose tissue in mice and humans. Cell Death & Disease, 10(6), 468.

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Quantifying Pol II-pCDK9 Interactions

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HELF or HEK293 cells growing in 100 mm plates were infected with different HCMV strains at an MOI of 1.0 or transfected with vectors transcribing RNA2.7C2c. PureProteome^TMProteinA/G Mix Magnetic Beads (Millipore, #LSKMAGAG10) were coated with anti-Pol II antibody (Millipore, #05-623-Z) and were incubated with lysates at 4 °C overnight. The captured protein complex was eluted with 60 μL SDS-PAGE sample loading buffer (Beyotime, Nantong, China, #P0015) and was then heated at 70 °C for 10 min. After the beads were removed, the supernatants were loaded on 8% SDS-PAGE gel. Blotted PVDF membranes were incubated with antibodies against Pol II and pCDK9, followed by peroxidase-conjugated goat anti-mouse or rabbit IgG (ZSGB-BIO, Guangzhou, China, #ZB-2305 or #ZB-2301) with ECL Western blot reagent (ThermoFisher). Protein relative densities were quantified using ImageJ software 1.44p (NIH). Pol II captured was measured as a quantitative reference to calculate the relative amount of pCDK9 binding to Pol II.

Huang Y., Guo X., Zhang J., Li J., Xu M., Wang Q., Liu Z., Ma Y., Qi Y, & Ruan Q. (2022). Human cytomegalovirus RNA2.7 inhibits RNA polymerase II (Pol II) Serine-2 phosphorylation by reducing the interaction between Pol II and phosphorylated cyclin-dependent kinase 9 (pCDK9). Virologica Sinica, 37(3), 358-369.

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