Masshunter workstation acquisition b 04

ESI mass spectra were recorded using an Agilent 6220 time-of-flight mass spectrometer (Agilent Technologies, Santa Clara, CA, USA) in extended dynamic range mode equipped with a Dual-ESI source, operating with a spray voltage of 2.5 kV. Nitrogen served both as the nebulizer gas and the dry gas. Nitrogen was generated by a nitrogen generator NGM 11. Samples are introduced with a 1200 HPLC system consisting of an autosampler, degasser, binary pump, column oven, and diode array detector (Agilent Technologies, Santa Clara, CA, USA) using a C18 Hypersil Gold column (length: 50 mm, diameter: 2.1 mm, particle size: 1.9 μm) with a short isocratic flow (60% B for 5 min) at a flow rate of 250 μL/min and column oven temperature of 40°C. HPLC solvent A consisted of 94.9% water, 5% acetonitrile, and 0.1% formic acid, solvent B of 5% water, 94.9% acetonitrile, and 0.1% formic acid. The mass axis was externally calibrated with ESI-L Tuning Mix (Agilent Technologies, Santa Clara, CA, USA) as calibration standard. The mass spectra were recorded in both profile and centroid mode with the MassHunter Workstation Acquisition B.04.00 software (Agilent Technologies, Santa Clara, CA, USA). MassHunter Qualitative Analysis B.07.00 software (Agilent Technologies, Santa Clara, CA, USA) was used for processing and averaging of several single spectra.

Phenolic compounds contained in aqueous extract (extracts used by the traditional healers), hydroethanolic extract (most active crude extract), and ethyl acetate fraction (most active fraction) were quantified using analytical LC-MS. The analysis was performed on an Agilent 6220 ToF-MS with a Dual ESI-source, 1200 HPLC system with autosampler, degasser, binary pump, column oven, diode array detector, and a Hypersil Gold C18 column (1.9 μm, 50 × 2.1 mm) with a gradient (in 11 min from 0% B to 98% B, back to 0% B in 0.5 min, total run time 15 min) at a flow rate of 300 μL/min and column oven temperature of 40°C. HPLC solvent A consists of 94.9% water, 5% acetonitrile and 0.1% formic acid, solvent B of 5% water, 94.9% acetonitrile, and 0.1% formic acid. The mass spectra are recorded in both profile and centroid mode with the MassHunter Workstation Acquisition B.04.00 software (Agilent Technologies, Santa Clara, CA, USA). MassHunter Qualitative Analysis B.07.00 software (Agilent Technologies, Santa Clara, CA, USA) was used for processing and averaging of several single spectra.