miR-130b-3p antagomir, antagomir-negative control (antagomir-NC), miR-130b-3p mimic and the mimic negative control (mimic-NC) were purchased from GenePharma Co. Ltd (Shanghai, China). All the bases were 2’-O-methy modified, and the 3’-end was conjugated to cholesterol. All the sequence was as the followings: antagomir -miR-130b-3p: 5′-UGCCAACCUUGCAAGCCGAAG-3′ antagomir-NC: 5′-CAGUACUUUUGUGUAGUACAA-3′ miR-130b-3p mimic: 5′-AAGGGCGUUGGAAUCGGCU-3′ miR-130b-3p mutant: 5′-GACCAACUUUGGCCUGAACCUCCU-3′. Mimic control: 5′-CAAGUACUUUGUGUGUAGUACAA-3′. Cells were transfected with the antagomir or mimic at a concentration of 100 nM /ml. The transfection was performed utilizing Lipofectamine 3000 (Invitrogen).
Mir 130b 3p antagomir
Manufactured by GenePharma
MiR-130b-3p antagomir is a short, synthetic RNA molecule designed to inhibit the activity of the miR-130b-3p microRNA. MicroRNAs are small, non-coding RNA molecules that play important roles in gene expression regulation.
Automatically generated - may contain errors
Lab products found in correlation
Mimic nc, by GenePharma (1 mentions)
Lipofectamine 3000, by Thermo Fisher Scientific (1 mentions)
Mir 130b 3p mimic, by GenePharma (1 mentions)
Control sirna, by GenePharma (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Mir 130b 3p inhibitor, by GenePharma (1 mentions)
Lipofectamine 2000 reagent, by Thermo Fisher Scientific (1 mentions)
Control sirna, by Thermo Fisher Scientific (1 mentions)
Pten sirna, by Thermo Fisher Scientific (1 mentions)
Pten sirna, by Santa Cruz Biotechnology (1 mentions)
2 protocols using mir 130b 3p antagomir
1
miR-130b-3p Regulation in Cells
2
Nephroblastoma Cell Line WiT49 Transfection
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Nephroblastoma cell line WiT49 was provided by The Hospital for Sick Children (Toronto, Canada) and was subsequently grown in Dulbecco's Modified Eagle's Medium/Nutrient Mixture F-12 (Invitrogen; Thermo Fisher Scientific, Inc.) containing 10% fetal bovine serum (FBS; Invitrogen; Thermo Fisher Scientific, Inc.) and 1% penicillin streptomycin. These cells were cultured in a humidified chamber at 37°C with 5% CO2. miR-130b-3p inhibitor (miR-130b-3p antagomir; sequence: 5′-UGCCAACCUUGCAAGCCGAAG-3′) and inhibitor control (antagomir-negative control; sequence: 5′-CAGUACUUUUGUGUAGUACAA-3′) were purchased from GenePharma Co. Ltd. WiT49 cells were transfected with either 100 nM inhibitor control, 100 nM miR-130b-3p inhibitor, 2 µl control-siRNA (cat. no. Sc-36869; Santa Cruz Biotechnology, Inc., Dallas, TX, USA), 2 µl PTEN-siRNA (cat. no. Sc-29459; Santa Cruz Biotechnology, Inc.), 100 nM miR-130b-3p inhibitor + 2 µl control-siRNA or 100 nM miR-130b-3p inhibitor + 2 µl PTEN-siRNA by using the Lipofectamine® 2000 reagent (Invitrogen; Thermo Fisher Scientific, Inc.) according to the manufacturer's protocol. Cells without any treatment were considered as the control group. The cells were subjected to the following experiments 48 h after transfection.
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