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2 protocols using doxycycline

1

Antibiotic Susceptibility of Sorbitol-Negative Strains

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The sorbitol-negative strains were examined for resistance against ampicillin (10 µg), cephalothin (30 µg), ceftazidime (30 µg), gentamicin (10 µg), doxycycline (30 µg), ciprofloxacin (5 µg), nalidixic acid (30 µg), cotrimoxazole (25 µg), and chloramphenicol (30 µg) (MAST Group Ltd., UK), using Kirby-Bauer disc diffusion susceptibility test and the characterization of strains as susceptible, reduced susceptibility or resistant was as recommended by the Clinical and Laboratory Standards Institute (CLSI) (14 ).
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2

Antimicrobial Susceptibility Testing Protocol

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Antimicrobial susceptibility testing was carried out by the Kirby–Bauer disc diffusion method according to Clinical and Laboratory Standards Institute (CLSI) [12 ] and breakpoint interpretations for including amikacin (30 µg), gentamicin (10 µg), tobramycin (10 µg), cotrimoxazole (trimethoprim–sulfamethoxazole) (25 µg), tigecycline (15 µg), doxycycline (30 µg), tetracycline (30 µg), minocycline (30 µg) ceftriaxone (30 µg), ceftazidime (30 µg), cefepime (30 µg), cefotaxime (30 µg), piperacillin/tazobactam (100/10 µg), ampicillin-sulbactam (10/10 µg), imipenem (10 µg), meropenem (10 µg), ciprofloxacin (5 µg), levofloxacin (5 µg), (Mast Group Ltd., Bootle, UK). The minimum inhibitory concentrations (MICs) of colistin (Colistin sulfate salt powder, Sigma-Aldrich, St. Louis, MO, USA) were determined using the broth microdilution method according to CLSI guidelines (CLSI, 2020). Escherichia coli ATCC 25922 and Pseudomonas aeruginosa ATCC 27,853 strains were used as the standard strains.
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