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Hrp conjugated goat anti mouse or rabbit igg

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HRP-conjugated goat anti-mouse or rabbit IgG is a secondary antibody conjugated with horseradish peroxidase (HRP). It is used to detect and quantify mouse or rabbit primary antibodies in various immunoassay techniques.

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Lab products found in correlation

Surebeads protein a magnetic beads, by Bio-Rad (2 mentions) Veriblot, by Abcam (2 mentions)

2 protocols using hrp conjugated goat anti mouse or rabbit igg

1

Identifying CatSper Protein Interactions

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Solubilized testis microsome and round spermatids were subjected to coIP. Solubilized proteins in lysis buffer were mixed with SureBeads Protein A Magnetic Beads (BioRad) conjugated with either 1 μg of rabbit polyclonal anti-CatSper1, CatSperδ, or CatSperτ(α-CST-482), or 0.5 μL of rabbit monoclonal anti-DYKDDDDK (clone D6W5B, CST). After incubation for overnight at 4°C, the resins were washed with lysis buffer and eluted immunocomplexes were eluted with 2X LDS buffer supplemented with 50 μM DTT by boiling at 75°C for 10 min. The elutes were subjected to SDS-PAGE and immunoblotting. Primary antibodies for immunoblotting were: rabbit polyclonal anti-mouse CatSper1, CatSper2, CatSper3, CatSperβ, CatSperδ, CatSperɛ, and CatSperτ at 1 μg/mL, and rabbit monoclonal anti-DYKDDDDK (1:2,000; clone D6W5B, CST). For secondary antibodies, VeriBlot (1:200-1:500; Abcam) and HRP-conjugated goat anti-mouse or rabbit IgG (1:10,000; Jackson ImmunoResearch) were used.
Hwang J.Y., Wang H., Lu Y., Ikawa M, & Chung J.J. (2022). C2cd6-encoded CatSperτ targets sperm calcium channel to Ca2+ signaling domains in the flagellar membrane. Cell reports, 38(3), 110226.
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2

Identifying CatSper Protein Interactions

Check if the same lab product or an alternative is used in the 5 most similar protocols
Solubilized testis microsome and round spermatids were subjected to coIP. Solubilized proteins in lysis buffer were mixed with SureBeads Protein A Magnetic Beads (BioRad) conjugated with either 1 μg of rabbit polyclonal anti-CatSper1, CatSperδ, or CatSperτ(α-CST-482), or 0.5 μL of rabbit monoclonal anti-DYKDDDDK (clone D6W5B, CST). After incubation for overnight at 4°C, the resins were washed with lysis buffer and eluted immunocomplexes were eluted with 2X LDS buffer supplemented with 50 μM DTT by boiling at 75°C for 10 min. The elutes were subjected to SDS-PAGE and immunoblotting. Primary antibodies for immunoblotting were: rabbit polyclonal anti-mouse CatSper1, CatSper2, CatSper3, CatSperβ, CatSperδ, CatSperɛ, and CatSperτ at 1 μg/mL, and rabbit monoclonal anti-DYKDDDDK (1:2,000; clone D6W5B, CST). For secondary antibodies, VeriBlot (1:200-1:500; Abcam) and HRP-conjugated goat anti-mouse or rabbit IgG (1:10,000; Jackson ImmunoResearch) were used.
Hwang J.Y., Wang H., Lu Y., Ikawa M, & Chung J.J. (2022). C2cd6-encoded CatSperτ targets sperm calcium channel to Ca2+ signaling domains in the flagellar membrane. Cell reports, 38(3), 110226.
+ Open protocol
+ Expand

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