Bathophenanthrolinedisulphonic acid

Manufactured by Merck Group

Sourced in United States

Bathophenanthrolinedisulphonic acid is a chemical compound used in laboratory settings. It is a chelating agent that forms stable complexes with various metal ions. The core function of this compound is to facilitate the detection and measurement of these metal ions through colorimetric or fluorometric analysis.

Automatically generated - may contain errors

Lab products found in correlation

Multigent iron kit, by Abbott (2 mentions) Microtainer sst tube, by BD (1 mentions) Architect c16000 automated analyzer, by Abbott (1 mentions) 2 4 6 trinitrobenzenesulphonic acid, by Merck Group (1 mentions) 3 2 pyridyl 5 6 diphenyl 1 2 4 triazine 4 4 disulfonic acid sodium salt ferrozine, by Merck Group (1 mentions) Bovine serum albumin (bsa), by Merck Group (1 mentions) 6 hydroxy 2 5 7 8 tetramethylchroman 2 carboxylic acid, by Merck Group (1 mentions) Thioglycolic acid, by Merck Group (1 mentions) Ferric ammonium citrate, by Merck Group (1 mentions) Pentra c400, by Horiba (1 mentions) Architect c16000, by Abbott (1 mentions)

3 protocols using bathophenanthrolinedisulphonic acid

Non-heme Iron Content Determination

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Tissues were dried for 3 hours at 100°C, and the dry mass of tissue piece was measured. Tissue pieces were digested in 10% trichloroacetic acid/ 30% hydrochloric acid for 20 hours at 65°C. Non-haem iron content was determined by reaction of the acid digested tissue with chromogen reagent containing 0.1% (w/v) bathophenanthrolinedisulphonic acid (Sigma, 146617) and 0.8% thioglycolyic acid (Sigma, 88652) measuring absorption at 535nm and comparing to a standard curve of ferric ammonium citrate as described.⁵⁴ (link)
For serum analysis of murine samples up to 400μl of blood obtained by cardiac puncture was placed in a BD microtainer SST tube (Beckton Dickinson). Serum was obtained by spinning the clotted blood sample was spun at 8,000 g for 5 minutes and stored at −80°C. Serum iron was quantified using the Abbott Architect c16000 automated analyzer (Abbott Laboratories) and the MULTIGENT Iron Kit at Oxford John Radcliffe Hospital, UK.

Frost J.N., Tan T.K., Abbas M., Wideman S.K., Bonadonna M., Stoffel N.U., Wray K., Kronsteiner B., Smits G., Campagna D.R., Duarte T.L., Lopes J.M., Shah A., Armitage A.E., Arezes J., Lim P.J., Preston A.E., Ahern D., Teh M., Naylor C., Salio M., Gileadi U., Andrews S.C., Dunachie S.J., Zimmermann M.B., van der Klis F.R., Cerundolo V., Bannard O., Draper S.J., Townsend A.R., Galy B., Fleming M.D., Lewis M.C, & Drakesmith H. (2021). Hepcidin-Mediated Hypoferremia Disrupts Immune Responses to Vaccination and Infection. Med (New York, N.y.), 2(2), 164-179.e12.

+ Open protocol

+ Expand

Antioxidant Assay Reagents Procurement

Check if the same lab product or an alternative is used in the 5 most similar protocols

Bathophenanthrolinedisulphonic acid, 2,2′-azinobis (3-thylbenzothiazoline-6-sulfonic acid) (ABTS), 6-hydroxy-2,5,7,8-tetramethyl-chroman-2-carboxylic acid (Trolox), 2,4,6-trinitrobenzenesulphonic acid (TNBS), and 3-(2-pyridyl)-5,6-diphenyl-1,2,4-triazine-4′,4′′-disulfonic acid sodium salt (ferrozine) were purchased from Sigma-Aldrich Chemical Co. (St. Louis, MO, USA). Bovine serum albumin (BSA) was obtained from Sigma Aldrich Chemical Co. (St. Louis, MO, USA) and hexane was from Macron Fine Chemicals TM (Dublin, Ireland). All chemicals used in this study were of analytical grade.

Kingwascharapong P., Chaijan M, & Karnjanapratum S. (2021). Ultrasound-assisted extraction of protein from Bombay locusts and its impact on functional and antioxidative properties. Scientific Reports, 11, 17320.

+ Open protocol

+ Expand

Quantifying Non-Heme Liver Iron Levels

Check if the same lab product or an alternative is used in the 5 most similar protocols

Serum iron measurements were performed on an Abbott Architect c16000 automated analyser by Oxford University Hospitals Clinical Biochemistry staff using the MULTIGENT Iron Kit (Abbott), or using a Pentra C400 automated analyser with the Iron CP ABX Pentra Kit (HORIBA Medical).
Non-haem liver iron measurements were performed as previously described [88 (link)]. In short, pieces of liver tissue were collected, snap-frozen, and stored at -80° C. The tissue was dried at 100° C for ~6 h, weighed, and then digested in 10% trichloroacetic acid / 30% hydrochloric acid in water for ~20 hours at 65°C. Subsequently, a chromogen reagent containing 0.1% bathophenanthrolinedisulphonic acid (Sigma, 146617) / 0.8% thioglycolic acid (Sigma, 88652) / 11% sodium acetate in water was added, and the absorbance at 535 nm measured. The iron content was determined by comparing the samples against a standard curve of serially diluted ammonium ferric citrate (F5879, Merck).

Wideman S.K., Frost J.N., Richter F.C., Naylor C., Lopes J.M., Viveiros N., Teh M.R., Preston A.E., White N., Yusuf S., Draper S.J., Armitage A.E., Duarte T.L, & Drakesmith H. (2023). Cellular iron governs the host response to malaria. PLOS Pathogens, 19(10), e1011679.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!