Np23 pe

Manufactured by Biosearch Technologies

The NP23-PE is a laboratory instrument produced by Biosearch Technologies. It is designed for the detection and analysis of biological samples. The core function of the NP23-PE is to perform fluorescence-based measurements.

Automatically generated - may contain errors

Lab products found in correlation

Ebio7h22 e16, by Biosearch Technologies (2 mentions) Np25 pe, by Biosearch Technologies (2 mentions) Gallios cytometer, by Beckman Coulter (1 mentions) Facsaria 2 cytometer, by BD (1 mentions) Apc cy7, by BioLegend (1 mentions) Alexa fluor 700, by BioLegend (1 mentions) Pe dazzle 594, by BioLegend (1 mentions) Propidium iodide, by Merck Group (1 mentions) Fluorophore labeled antibodies, by BioLegend (1 mentions) Facsverse cytometer, by BD (1 mentions)

6 protocols using np23 pe

Flow Cytometry Analysis of Mouse B Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

A single-cell suspension was prepared by passing tissues through a 100-μm nylon cell strainer. Red blood cell lysis was performed in lysis buffer (150 mM NH₄Cl, 14 mM NaHCO₃, and 2 mM EDTA). The following antibodies and reagents were used for flow cytometry: anti-CD19 allophycocyanin (APC)-Cy7 (BioLegend, #6D5, 0.25 μg/ml), anti-CD38 phycoerythrin (PE)-Dazzle594 (BioLegend, #90, 0.5 μg/ml), anti-CD45R (B220) PE-Cy7 (BioLegend, #RA3-6B2, 0.25 μg/ml), anti-CD95 APC (Miltenyi Biotec, #REA453, 0.15 μg/ml), anti-IgG1 fluorescein isothiocyanate (FITC) (BD Biosciences, #A85–1, 0.5 μg/ml), anti-Ig light chain κ (Igκ) Alexa Fluor 700 (BioLegend, #RMK-45, 1.25 μg/ml), NP₂₃ PE (LGC Biosearch Technologies, 0.5 μg/ml), and propidium iodide (Sigma-Aldrich, 1 μg/ml). A flow cytometric analysis was performed on a Gallios cytometer (Beckman Coulter). Cell sorting was performed on a FACSAria II cytometer (BD Biosciences).

Sugiyama Y., Fujiwara M., Sakamoto A., Tsushima H., Nishikimi A, & Maruyama M. (2022). The immunosenescence-related factor DOCK11 is involved in secondary immune responses of B cells. Immunity & Ageing : I & A, 19, 2.

+ Open protocol

+ Expand

Flow Cytometry of Lymphoid Populations

Check if the same lab product or an alternative is used in the 5 most similar protocols

Streptavidin (SA) and antibodies to B220 (RA3-6B2), BAFFR/CD268 (eBio7H22-E16), CD3 (17A2), CD4 (RM4-5 or GK1.5), CD8 (53-6.7), CD19 (1D3), CD21 (7G6), CD23 (B3B4), CD86 (GL-1), CD62L (MEL-14), CD69 (H1.2F3), CD93 (AA4.1), CD44 (IM7), CD45.1 (A20), CD45.2 (104), IgM (II/41), IgD (11-26c), Igλ1 (R11-153) conjugated to biotin or fluorophores (BioLegend, eBiosciences, BD, or Tonbo); NP(23)-PE (LGC Biosearch Technologies).

Tan C., Hiwa R., Mueller J.L., Vykunta V., Hibiya K., Noviski M., Huizar J., Brooks J.F., Garcia J., Heyn C., Li Z., Marson A, & Zikherman J. (2020). NR4A nuclear receptors restrain B cell responses to antigen when second signals are absent or limiting. Nature immunology, 21(10), 1267-1279.

+ Open protocol

+ Expand

NP-Specific B Cell Staining Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

To stain for NP-specific B cells, NP25-PE or NP23-PE was used at 1:200 dilution (LGC Biosearch Technologies).

Brooks J.F., Tan C., Mueller J.L., Hibiya K., Hiwa R., Vykunta V, & Zikherman J. (2021). Negative feedback by NUR77/Nr4a1 restrains B cell clonal dominance during early T-dependent immune responses. Cell reports, 36(9), 109645.

+ Open protocol

+ Expand

Multiparametric Flow Cytometry Profiling

Check if the same lab product or an alternative is used in the 5 most similar protocols

Single cells were stained with fluorophore-labeled antibodies recognizing the following molecules (BioLegend): B220 (RA3-6B2), CD4 (RM4-5), CD8α (53–6.7), CD19 (6D5), IgD (11-26c.2a), IgG1 (RMG1-1), IgM (RMM-1), Ly-6G (1A8), PD-1 (29F.1A12), and TCRβ (H57-597). Antibodies against CD95 (Jo2) and CXCR5 (2G8) were purchased from BD. Data were acquired on a FACSVerse cytometer (BD) and analyzed with FlowJo software (Tree Star) to detect the following populations: B cells in blood and lymph (CD19⁺) or LNs (CD19⁺IgD^hiCD95⁻) of unimmunized mice; CD4⁺ or CD8⁺ T cells (CD4⁺ or CD8⁺) in blood, lymph, and LNs of unimmunized mice; and GC B cells (B220⁺IgD^loCD95⁺IgM⁺ or IgG1⁺) and Tfh cells (CD4⁺TCRβ⁺CXCR5⁺PD-1⁺) in LNs of immunized mice. NP-specific GC B cells were labeled with NP₂₃-PE (Biosearch Technologies) at 7 and 14 d after immunization.

Suzuki K., Hayano Y., Nakai A., Furuta F, & Noda M. (2016). Adrenergic control of the adaptive immune response by diurnal lymphocyte recirculation through lymph nodes. The Journal of Experimental Medicine, 213(12), 2567-2574.

+ Open protocol

+ Expand

Flow Cytometry of Lymphoid Populations

Check if the same lab product or an alternative is used in the 5 most similar protocols

+ Open protocol

+ Expand

NP-Specific B Cell Staining Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

To stain for NP-specific B cells, NP25-PE or NP23-PE was used at 1:200 dilution (LGC Biosearch Technologies).

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!