Alantolactone (HY-N0038) was obtained from MedChem Express (MCE). Recombinant mouse IL-1β cytokine was provided from R&D Systems (501-RL-010, United States). Solarbio supplied safranin O liquor (Beijing, China). Primary antibodies applied in this research were: anti-iNOS, anti-MMP-13 which were acquired from Abcam (Shanghai, China), anti-COX2, anti-LC3Ⅱ/Ⅰ, anti-P-STAT3, anti-P-P65, anti-P-IκB, anti-P/T-mTOR which were purchased from CST (Beverly, MA, United States), anti-STAT3, anti-MMP-1, anti-MMP-3, anti-ATG5, anti-P62, anti-P65, anti-IκB, anti-P/T-PI3K, anti-P/T-AKT, anti-GAPDH which were afforded from Proteintech Group (Wuhan, Hubei, China) and anti-ADAMTS5 which was supplied from Boster Biological Technology (Wuhan, Hubei, China). Secondary antibodies, Cy3 and FITC Conjugated AffiniPure Goat Anti-Rabbit IgG, DAPI staining solution, collagenase type II and trypsin were got from Boster Biological Technology (Wuhan, Hubei, China). HanBio Inc. (Shanghai, China) served the mRFP-GFP-LC3 adenoviral vectors.
Alantolactone
Manufactured by MedChemExpress
Sourced in United States
Alantolactone is a sesquiterpene lactone compound that can be used as a chemical standard or reference material. It is extracted from the root of the plants Inula helenium or Aucklandia lappa. Alantolactone has a molecular formula of C₁₅H₂₀O₂ and a molecular weight of 232.32 g/mol.
Automatically generated - may contain errors
Lab products found in correlation
Trypsin, by Boster Bio (1 mentions)
Anti mmp13, by Abcam (1 mentions)
Anti p65, by Proteintech (1 mentions)
Anti stat3, by Proteintech (1 mentions)
Dapi staining solution, by Boster Bio (1 mentions)
Anti iκb, by Proteintech (1 mentions)
Collagenase type 2, by Boster Bio (1 mentions)
Anti mmp1, by Proteintech (1 mentions)
Anti mmp 3, by Proteintech (1 mentions)
Anti atg5, by Proteintech (1 mentions)
Anti p62, by Proteintech (1 mentions)
Anti inos, by Abcam (1 mentions)
Anti gapdh, by Proteintech (1 mentions)
Rapamycin, by MedChemExpress (1 mentions)
Bv173, by National Collection of Authenticated Cell Cultures (1 mentions)
Nalm6, by National Collection of Authenticated Cell Cultures (1 mentions)
Nalm1, by National Collection of Authenticated Cell Cultures (1 mentions)
Supb15, by National Collection of Authenticated Cell Cultures (1 mentions)
2 protocols using alantolactone
1
Alantolactone Modulates Inflammatory Response
2
Culturing Human Leukemia Cell Lines
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The human acute lymphoblastic leukemia cell lines (BV173, NALM6, JM-1, NALM1, RS6 and SUPB15) were purchased from the Cell Bank of the Chinese Academy of Sciences (Shanghai, China) . All cell lines were cultured in Dulbecco's altered Eagle's medium (DMEM) supplemented with 10% fetal bovine serum (FBS) (Hyclone, USA) at 37℃ with 5% CO 2 in a humidi ed incubator. Alantolactone and rapamycin were obtained from MedChemExpress (New Jersey, USA). Both the tested compounds were dissolved in dimethylsulfoxide (DMSO). The nal concentration of DMSO in culture medium did not exceed 0.1%.
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